Developmental emergence of two-stage nonlinear synaptic integration in cerebellar interneurons
- Sorbonne Université et CNRS UMR 8256, Adaptation Biologique et Vieillissement 9 Quai 10 St Bernard, France
- Department of Neuroscience, Institut Pasteur, CNRS URA 21821, 25 rue du Dr Roux, France
- Institute of Science and Technology Austria, Am Campus 1, Austria
- Paris Brain Institute, CNRS UMR 7225 - Inserm U1127 – Sorbonne Université, Groupe 12 Hospitalier Pitié Salpêtrière 47 Boulevard de l'Hôpital, France
Figures
Figure 1
Developmental maturation of the AMPA receptor (AMPAR)-mediated miniature excitatory postsynaptic current (mEPSC) in stellate cell (SC).
(A) Left panels show superimposed single mEPSCs (gray) and the corresponding average (bold) recorded at −70 mV obtained from a representative immature SCs (P17, blue trace) and adult SCs (P44, green …
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Figure 1—source data 1
Developmental maturation of the AMPAR-mediated mEPSC in SC.
- https://cdn.elifesciences.org/articles/65954/elife-65954-fig1-data1-v1.xlsx
Figure 2
Numerical simulations of stellate cell (SC) dendrites indicate significant cable filtering and large local depolarizations in immature SC.
(A) Maximal intensity projection of one-photon confocal images of an immature SC labeled with Alexa 488. Examples of intensity profiles (yellow line) of three dendritic locations from proximal to …
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Figure 2—source data 1
Numerical simulations of SC dendrites indicate significant cable filtering and large local depolarizations in immature SC.
- https://cdn.elifesciences.org/articles/65954/elife-65954-fig2-data1-v1.xlsx
Figure 3
Quantal excitatory postsynaptic current (EPSC) properties and postsynaptic density (PSD) areas for synapses contacting the soma and dendrites of immature stellate cell (SC).
(A) Diagram of a parasagittal cerebellar slice showing parallel fibers (PFs) projecting perpendicular (blue dots) to the dendritic plane of SCs (in red), allowing precise positioning of the stimulus …
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Figure 3—source data 1
qEPSC properties and PSD areas for synapses contacting the soma and dendrites of immature SC.
- https://cdn.elifesciences.org/articles/65954/elife-65954-fig3-data1-v1.xlsx
Figure 4
Developmental changes in somatic quantal excitatory postsynaptic current (qEPSC) properties and somatic postsynaptic density (PSD) size.
(A) The left panel shows a diagram of a parasagittal cerebellar slice showing parallel fibers (PFs) projecting perpendicular (blue dots) to the dendritic plane of a stellate cell (SC) (in red), …
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Figure 4—source data 1
Developmental changes in somatic qEPSC properties and somatic PSD size.
- https://cdn.elifesciences.org/articles/65954/elife-65954-fig4-data1-v1.xlsx
Figure 5
PSD95 puncta distribution on immature and adult stellate cell (SC).
(A) Maximum intensity projection of merged images showing a P14 (top) and P42 (bottom) SC labeled with Alexa 594 (magenta) and Venus-tagged PSD95 puncta (green). (B) Example of single optical …
Figure 6
PSD95 puncta distribution and morphological analysis of immature and adult stellate cell (SC).
(A) Cumulative plot showing Venus-tagged PSD95 puncta distributions for nine immature (blue) and eight adult (green) SCs. The bold trace is the population average (shaded region indicates SEM), …
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Figure 6—source data 1
PSD95 puncta distribution and morphological analysis of immature and adult SC.
- https://cdn.elifesciences.org/articles/65954/elife-65954-fig6-data1-v1.xlsx
Figure 7
The developmental change in synaptic distribution recapitulates the developmental change in miniature excitatory postsynaptic current (mEPSC) properties.
(A) Numerical simulations of somatic and dendritic quantal excitatory postsynaptic currents (qEPSCs) for synapses placed on the soma (red dot) and at 10 μm intervals (blue dots) along seven of the …
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Figure 7—source data 1
The developmental change in synaptic distribution recapitulates the developmental change in mEPSC properties.
- https://cdn.elifesciences.org/articles/65954/elife-65954-fig7-data1-v1.xlsx
Figure 8
Location dependence of short-term plasticity and sublinear behavior in immature stellate cell (SC).
(A) Single-trial excitatory postsynaptic current responses (eEPSCs, gray) and their corresponding average (bold) evoked by a pair of electrical stimuli (at 50 Hz).The stimulation electrode was …
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Figure 8—source data 1
Location dependence of short-term plasticity and sublinear behavior in immature SC.
- https://cdn.elifesciences.org/articles/65954/elife-65954-fig8-data1-v1.xlsx
Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus – male and female) | CB6F1 | Mouse Genome Informatics | RRID:MGI:5649749 | |
| Strain, strain background (Mus musculus – male and female) | PSD-95-CreNABLED mice | Haining Zhong, Vollum Institute | DOI: https://doi.org/10.1523/JNEUROSCI.3888-14.2014 | |
| Strain, strain background (Mus musculus – male and female) | B6.129-Nos1tm1(cre)Mgmj/J | Mouse Genome Informatics | https://www.jax.org/strain/017526 | |
| Chemical compound, drug | Gabazine (SR-95531) | Abcam | Cat#:ab120042 | |
| Chemical compound, drug | QX-314 | Abcam | Cat#:ab120118 | |
| Chemical compound, drug | 7-Chlorokynurenic acid | Abcam | Cat#:ab120255 | |
| Chemical compound, drug | Alexa Fluor 488 | ThermoFisher Scientific | Cat#:A10436 | |
| Chemical compound, drug | Alexa Fluor 594 | ThermoFisher Scientific | Cat#:A10438 | |
| Chemical compound, drug | D-AP5 | Abcam | Cat#:ab120003 | |
| Chemical compound, drug | Gamma DGG | Abcam | Cat#:ab120307 | |
| Chemical compound, drug | TTX | Abcam | Cat#:Ab120055 | |
| Chemical compound, drug | Strychnine | Sigma | Cat#:S0532 | |
| Software, algorithm | Reconstruct | JC Fiala | DOI: 10.1111 /j.1365–2818.2005.01466 .x | |
| Software, algorithm | Igor Pro | Wavemetrics | RRID:SCR_000325 | |
| Software, algorithm | Neuromatic | Rothman and Silver, 2018; DOI:10.3389 | RRID:SCR_004186 | |
| Software, algorithm | GraphPad Prism 6 | GraphPad Software | RRID:SCR_002798 | |
| Software, algorithm | ImageJ | National Institutes of Health | RRID:SCR_003070 | |
| Software, algorithm | Imaris | Bitplane Imaris Software | RRID:SCR_007370 | |
| Software, algorithm | Zeiss Atlas | Zeiss | https://www.zeiss.com/microscopy/int/products/microscope-software/atlas.html |
