The illustration shows the levels of organization of a plant (left) and a few examples highlight how location determines function at each level (right). At the molecular level, functions of protein complexes can be determined by their localization in membrane microdomains (Jarsch et al., 2014) or by dynamic protein interactions (Obata, 2019). At the cellular level, a protein can be located differentially through transport mechanisms, including vesicle trafficking (Goring and Di Sansebastiano, 2018) and nuclear translocation (Marchive et al., 2013), regulating its function. At the tissue level, cell position can drive its fate into a specialized cell type (Shao and Dong, 2016). Metabolic pathways can operate specifically in specialized cell types across tissues (Marchive et al., 2013; Schlüter and Weber, 2020). At the next level, the existence of non-cell-autonomous transcription factors can transverse intercellular scales across plant organs (Han et al., 2014). Also, an organ-dependent post-translational proteome has been described as a mechanism of protein function regulation (Uhrig et al., 2019). At the organism level, plant interaction with biotic (Harrison et al., 2002) and abiotic factors (Michaud et al., 2017) can occur through a localized cue perception.