Abstract

The molecular mechanisms underlying the diversity of cortical glutamatergic synapses is still incompletely understood. Here, we tested the hypothesis that presynaptic active zones (AZs) are constructed from molecularly uniform, independent release sites (RSs), the number of which scales linearly with the AZ size. Paired recordings between hippocampal CA1 pyramidal cells and fast-spiking interneurons in acute slices from adult mice followed by quantal analysis demonstrate large variability in the number of RSs (N) at these connections. High resolution molecular analysis of functionally characterized synapses reveals variability in the content of one of the key vesicle priming factors – Munc13-1 – in AZs that possess the same N. Replica immunolabeling also shows a 3-fold variability in the total Munc13-1 content of AZs of identical size, and a 4-fold variability in the size and density of Munc13-1 clusters within the AZs. Our results provide evidence for quantitative molecular heterogeneity of RSs and support a model in which the AZ is built up from variable numbers of molecularly heterogeneous, but independent RSs.

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Article and author information

Author details

  1. Maria Rita Karlocai

    Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Budapest, Hungary
    Competing interests
    The authors declare that no competing interests exist.
  2. Judit Heredi

    Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Budapest, Hungary
    Competing interests
    The authors declare that no competing interests exist.
  3. Tünde Benedek

    Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Budapest, Hungary
    Competing interests
    The authors declare that no competing interests exist.
  4. Noemi Holderith

    Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Budapest, Hungary
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-0024-3980
  5. Andrea Lorincz

    Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Budapest, Hungary
    Competing interests
    The authors declare that no competing interests exist.
  6. Zoltan Nusser

    Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Budapest, Hungary
    For correspondence
    nusser.zoltan@koki.mta.hu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-7004-4111

Funding

European Research Council (ERC-AG 787157)

  • Zoltan Nusser

Hungarian National Brain Research grant

  • Zoltan Nusser

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Linda Overstreet-Wadiche, University of Alabama at Birmingham, United States

Ethics

Animal experimentation: All the experiments were carried out according to the regulations of the Hungarian Act of Animal Care and Experimentation 40/2013 (II.14) and were reviewed and approved by the Animal Committee of the Institute of Experimental Medicine, Budapest.

Version history

  1. Received: February 11, 2021
  2. Accepted: April 26, 2021
  3. Accepted Manuscript published: April 27, 2021 (version 1)
  4. Version of Record published: May 12, 2021 (version 2)

Copyright

© 2021, Karlocai et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Maria Rita Karlocai
  2. Judit Heredi
  3. Tünde Benedek
  4. Noemi Holderith
  5. Andrea Lorincz
  6. Zoltan Nusser
(2021)
Variability in the Munc13-1 content of excitatory release site
eLife 10:e67468.
https://doi.org/10.7554/eLife.67468

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https://doi.org/10.7554/eLife.67468

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