Antibody | Podoplanin (T1-α) monoclonal PE-Cyanine7 | eBioscience/Thermo Fisher Scientific | RRID:AB_2573459 | Host: Syrian hamster0.125 µg/100 µl |
Antibody | CD31 (PECAM-1) FITC | eBioscience/Thermo Fisher Scientific | RRID:AB_465012 | Host: rat1 µg/100 µl |
Antibody | CD326 (EpCAM) monoclonal APC-eFluor780 | eBioscience/Thermo Fisher Scientific | RRID:AB_2573986 | Host: rat0.125 µg/100 µl |
Antibody | CD24 monoclonal, APC | eBioscience/Thermo Fisher Scientific | RRID:AB_10870773 | Host: rat0.06 µg/100 µl |
Antibody | CD45 monoclonal (30-F11), PE | eBioscience/Thermo fisher Scientific | RRID:AB_465668 | Host: rat0.125 µg/100 µl |
Antibody | TER-119 monoclonal, biotin | eBioscience/Thermo fisher Scientific | RRID:AB_466796 | Host: rat1:100 |
Antibody | CD16/32, clone 2.4G2, biotin | BD Biosciences | RRID:AB_394658 | Host: rat1:100 |
Antibody | CD104 [346-11A] biotin | BioLegend | RRID:AB_961034 | Host: rat1:100 |
Antibody | CD31 [MEC13.3] biotin | BioLegend | RRID:AB_312910 | Host: rat1:100 |
Antibody | CD45, biotin | eBioscience/Thermo Fisher Scientific | RRID:AB_466446 | Host: rat1:100 |
Antibody | Ki-67/MKI67 polyclonal | Novus Biologicals | RRID:AB_10001977 | Host: rabbit1:100 |
Antibody | Podoplanin polyclonal | R&D Systems | RRID:AB_2268062 | Host: goat15 µg/ml |
Antibody | Phospho-histone H2A.X (Ser139) | Cell Signaling Technology | RRID:AB_2118010 | Host: mouse1:100 |
Antibody | Pro-surfactant protein C (SFTP-C) | Millipore Sigma | RRID:AB_91588 | Host: rabbit1:1000 |
Antibody | Platelet-derived growth factor receptor-α | R&D Systems | RRID:AB_2236897 | Host: mouse1:50 |
Antibody | α-smooth muscle actin | American Research Product | RRID:AB_1540376 | Host: mouse1:500 |
Antibody | Goat anti-rabbit IgG secondary antibody-Alexa Fluor 594 | Thermo Fisher Scientific | RRID:AB_2556545 | Host: goat2 µg/ml |
Antibody | Donkey anti-goat IgG secondary antibody-Alexa Fluor 594 | Thermo Fisher Scientific | RRID:AB_2534105 | Host: donkey2 µg/ml |
Antibody | Donkey anti-mouse IgG secondary antibody-Alexa Fluor 594 | Thermo Fisher Scientific | RRID:AB_2556543 | Host: donkey2 µg/ml |
Antibody | Donkey anti-rabbit IgG secondary antibody-Alexa Fluor 488 | Thermo Fisher Scientific | RRID:AB_2556546 | Host: donkey2 µg/ml |
Antibody | Anti-Biotin MicroBeads UltraPure | Miltenyi Biotec | AB_2811216 | Host: mouse |
Chemical compound, drug | Fluorescent cell staining reagents | Fixable Viability Dye eFluor 450 | eBioscience/Thermo Fisher Scientific | |
Chemical compound, drug | Fluorescent cell staining reagents | Phalloidin-iFluor 594 Reagent -CytoPainter | Abcam | |
Chemical compound, drug | Fluorescent cell staining reagents | Hoechst 33342trihydrochloride trihydrate | Thermo Fisher Scientific | |
Chemical compound, drug | Bovine pituitary extract | Millipore Sigma | P1476-2.5ML | MTEC/plus supplement |
Chemical compound, drug | Collagenase, type 4 | Worthington Biochemical | LS004188 | L-MSC isolation |
Chemical compound, drug | Dispase II | Millipore Sigma | 4942078001 | AEC2 isolation |
Chemical compound, drug | Matrigel MembraneMatrix GFR | Corning | 356231 | Alveolosphere assay |
Chemical compound, drug | Insulin-transferrin-selenium-ethanolamine (ITS -X) (100X) | Thermo Fisher Scientific | 51500056 | MTEC/plus supplement |
Chemical compound, drug | Human insulin soln. | Millipore Sigma | I9278-5ML | MTEC/plus supplement |
Chemical compound, drug | EGF recombinantmouse protein | Thermo Fisher Scientific | PMG8041 | MTEC/plus supplement |
Chemical compound, drug | STEMRD Y27632ROCK inhibitor | Thermo Fisher Scientific | 50-175-997 | MTEC/plus supplement |
Chemical compound, drug | All-trans retinoic acid | Millipore Sigma | R2625-100MG | MTEC/plus supplement |
Chemical compound, drug | Cholera toxin | Millipore Sigma | C8052-5MG | MTEC/plus supplement |
Chemical compound, drug | Recombinant humanTGF-beta1 | PeproTech | 100-21-2ug | |
Chemical compound, drug | Horseradish peroxidase | Millipore Sigma | P8375-25KU | |
Chemical compound, drug | Homovanillic acid,fluorimetric reagent | Millipore Sigma | H1252-100MG | |
Chemical compound, drug | Hydrogen peroxide solution, 30% (w/w) | Millipore Sigma | H1009-100ML | |
Chemical compound, drug | Oligomycin | Millipore Sigma | O4876-5MG | |
Chemical compound, drug | Carbonyl cyanide 4 | Millipore Sigma | C2920-10MG | |
Chemical compound, drug | Antimycin A | Millipore Sigma | A8674-25MG | |
Chemical compound, drug | 2-deoxy D-glucose | Millipore Sigma | D6134-5G | |
Chemical compound, drug | Vectashield | Vector Laboratories | H-1400-10 | |
Chemical compound, drug | HistoGel; specimenprocessing gel | Richard-Allan Scientific/Thermo Fisher Scientific | HG-4000-012 | |
Commercial assay or kit | Pierce BCA ProteinAssay Kit | Thermo Fisher Scientific | PI23225 | |
Commercial assay or kit | EZQ Protein Quantitation Kit | Thermo Fisher Scientific | R33200 | |
Commercial assay or kit | MidiMACS Starting Kit (LS) | Miltenyi Biotec | 130-042-301 | |
Commercial assay or kit | Senescence DetectionKit | Abcam | Ab65351 | |
Commercial assay or kit | Mouse XL Cytokine Array Kit | R&D Systems | ARY028 | |
Commercial assay or kit | ADP/ATP Ratio Kit | Abcam | Ab65313 | |
Commercial assay or kit | Novex Colloidal Blue Staining kit | Invitrogen | LC6025 | |
Strain, strain background (Mus musculus) | C57BL/6J (2 months) male | Jackson Laboratory | IMSR_JAX:000664 | |
Strain, strain background (Mus musculus) | C57BL/6J (18 months) male | National Institute of Aging | N/A | |
Strain, strain background (Mus musculus) | C57BL/6J (Nox4 knockout) male | Dr. Karl-Heinz Krause | University of Geneva | |
Other | Mouse genotyping service | Transnetyx, Inc, Cordova, TN | | |
Chemical compound, drug | Isoflurane | VetOne | NDC 13985-528-60 | Anesthesia agent |
Other | Inverted microscope | Keyence | SCR_017202 | |
Other | Inverted microscope | Carl Zeiss | | |
Other | Nikon A1R confocal microscope | Nikon | | |
Other | Microplate reader | BioTek | SynergyMx | |
Other | Centrifuge | Eppendorf | 5702R | |
Other | Flow Cell Sorter | BD Biosciences | | |
Other | BD FACS Aria | BD Biosciences | SCR_018091 | |
Other | Flow Cell Analysis | BD Biosciences | BD LSR II GUAVA EasyCyte | |
Other | Extracellular Flux Analyzer (Seahorse XFe96) | Agilent | SCR_019545 | |
Other | LS columns (magnetic separation) | Miltenyi Biotec | 130-042-401 | AEC2 purification |
Other | Extracellular FluxAnalyzer | Agilent | Seahorse XFe96 | |
Software, algorithm | ImageJ | Wayne Rasband (NIH) | https//github.com/imagej/imagej1 | Johannes, 2021 |
Software, algorithm | Image Quant | Cytiva | Version TL8.1 | |
Software, algorithm | FlowJo | Tree Star Inc | | |
Software, algorithm | Nis Elements | Nikon | Version 5.0 | |
Sequence-based reagent | Mouse Nox4 forward | IDT | ACT TTT CAT TGG GCG TCC TC | RT-PCR |
Sequence-based reagent | Mouse Nox4 reverse | IDT | AGA ACT GGG TCC ACA GCA GA | RT-PCR |
Sequence-based reagent | Mouse β-actin forward | IDT | AGT GTG ACG TTG ACA TCC GT | RT-PCR |
Sequence-based reagent | Mouse β-actin reverse | IDT | TGC TAG GAG CCA GAG CAG TA | RT-PCR |