(A) Fraction of uninjured cells expressing selected genes in the macrophage cluster. snMac2 (blue), snMac1 (light blue), specific microglia genes (pink), CNS microglia/macrophages (green) and common macrophage markers (orange). (B) Heatmap of gene expression profile in macrophages by z-score for all injury conditions. (C) Pathway analysis (KEGG 2019) of differentially upregulated (red) and downregulated (blue) genes in the Macrophage cell cluster. n = 2 biologically independent experiments. (FDR ≤ 0.05, fold-change ≥ 2). (D) DRG qPCR analysis of DEG in macrophages after SNC compared to Naive. (E) Heatmap of M1, M2 macrophage markers, selected cytokines and proliferation marker gene expression by z- score for all injury conditions. (F) t-SNE plots of mouse DRG macrophages colored by injury condition and t-SNE overlay for expression of proliferation marker genes in pooled macrophage cluster from all injury conditions. (G) Representative images of immunofluorescence staining of DRG sections labeled with CD68 (green), MKI67 (white) and TUJ1 (red) from naïve mice, SNC, DRC and SCI injuries n = 5 biologically independent animals. Scale bar: 50 µm. (H) Quantification of area with CD68 expressing cells. (I) Quantification of MKI67 expressing cells normalized to DAPI. (J) Quantification of the percentage of cells expressing both MKI67 and CD68 out of all MKI67 positive cells. n = 5 (NAI,SNC,DRC) and n = 4 (SCI) biologically independent animals. (H-J) One-way analysis of variance (ANOVA) followed by Bonferroni’s multiple comparisons test. Data are presented as mean values ± SD. (K) Plot of cells expressing Mki67, colored by cell type, for all injury conditions. Every dot represents one cell. (6< log gene counts). (L) Quantification of the percentage of cells expressing Mki67, colored by cell type, in all injury conditions.