(A) Representative images showing co-expression of mCherry in the PVHvglut2 neurons (red) with c-fos immunostaining (green). The merged image is shown in the right panel (yellow). Scale bars: 200 …
C-fos expression and RV retrograde tracing of PVHvglut2 neurons.
Typical brain sections showing the source of monosynaptic afferents to PVHvglut2 neurons. The RV infection sites were marked by red rounds. Abbreviations of the brain regions used are as follows: …
(A) Schematic of the fiber photometry setup and in vivo recording configuration (DM dichroic mirror, PMT photomultiplier tube). (B) Unilateral viral targeting of AAV-EF1α-DIO-GCaMP6f into the PVH, …
In vivo fiber photometry and multichannel electrophysiological recordings of PVHvglut2 neurons.
(A, B) Expression of the AAV injection site in the PVH of vglut2Cre mice. Drawings of overlay mCherry expressing sites in the PVH of vglut2Cre mice (n = 7, indicated with different colors). (C) …
Time spent in each stage of PVHvglut2-M3 mice after administration of CNO or saline during the light phase.
Time spent in each stage of PVHvglut2 mice after administration of CNO or saline during the dark phase.
Heart rate, temperature, serum CRH and CORT levels of PVHvglut2-M3 mice.
(A) Time course of wakefulness, NREM sleep, and REM sleep following injection of vehicle or CNO during the dark phase in mice expressing hM3Dq receptor in PVHvglut2 neurons (n = 4). Statistical …
(A, B) Simultaneous monitoring of heart rate (A) and temperature (B) with implantable telemetry after administration of vehicle or CNO in freely moving mice. Left panel: Mean ± SEM of heart rate and …
(A) Left: Schematic of optogenetic manipulation of PVHvglut2 neurons and EEG/EMG recordings. Right: ChR2-mCherry expression and location of optical fiber in the PVH. Scale bar: 200 μm. (B) Example …
Latencies of transitions and time course in semi-chronic optogenetic experiments of PVHvglut2-ChR2 mice.
(A, E) Left: Schematic diagram showing the location of the optic fiber in the PB and LSv, and EEG/EMG recordings of a vglut2Cre mouse bilaterally injected with AAV-ChR2-mCherry or AAV-mCherry in the …
Statistical analysis of whole-brain outputs from PVHvglut2 neurons.
In vitro photostimulation-evoked EPSCs in PB and LSv neurons.
(A) Schematic of the viral vectors and injection site for AAV-hSyn-DIO-eGFP in vglut2Cre mice. (B) Fluorescence images showing the AAV infection area in the PVH of a vglut2Cre mouse. Scale bars: 200 …
(A, D) Left: Schematic diagram showing the location of the optic fiber in the NTS or PVT and EEG/EMG recordings of a vglut2Cre mouse injected with AAV-DIO-ChR2-mCherry or AAV-DIO-mCherry in the PVH. …
(A, D, G) Schematic drawings of the chemogenetic experiment in OTCre mice, PDYNCre mice and CRHCre mice. (B, E, H) Location of hM3Dq expression in PVHOT, PVHPDYN, and PVHCRH neurons. Right panel: …
Time spent in each stage of PVHOT-M3 mice, PVHPDYN-M3 mice, and PVHCRH-M3 mice after administration of CNO or saline.
(A) Expression of AAV injection site in the PVH of vglut2Cre mice. (B) Left panel: Location of hM4Di expression in the PVHvglut2 neurons. Right panel: Drawings of overlay mCherry expressing sites in …
Time spent in each stage of PVHvglut2-m4 mice after administration of CNO or saline and time spent in each stage of PVH vglut2-Caspase3 mice.