A 3D adult zebrafish brain atlas (AZBA) for the digital age
- Wayne State University, United States
- The Hospital for Sick Children, Canada
- Kansas State University, United States
- University of Toronto, Canada
Abstract
Zebrafish have made significant contributions to our understanding of the vertebrate brain and the neural basis of behavior, earning a place as one of the most widely used model organisms in neuroscience. Their appeal arises from the marriage of low cost, early life transparency, and ease of genetic manipulation with a behavioral repertoire that becomes more sophisticated as animals transition from larvae to adults. To further enhance the use of adult zebrafish, we created the first fully segmented three-dimensional digital adult zebrafish brain atlas (AZBA). AZBA was built by combining tissue clearing, light-sheet fluorescence microscopy, and three-dimensional image registration of nuclear and antibody stains. These images were used to guide segmentation of the atlas into over 200 neuroanatomical regions comprising the entirety of the adult zebrafish brain. As an open source, online (azba.wayne.edu), updatable digital resource, AZBA will significantly enhance the use of adult zebrafish in furthering our understanding of vertebrate brain function in both health and disease.
Data availability
Data have been deposited in Dryad, accessible at: https://doi.org/10.5061/dryad.dfn2z351g
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Data from: A 3D Adult Zebrafish Brain Atlas (AZBA) for the Digital AgeDryad Digital Repository, doi:10.5061/dryad.dfn2z351g.
Article and author information
Author details
Funding
Human Frontiers Science Program (LT000759/2014)
- Justin W Kenney
National Institutes of Health (R35GM142566)
- Justin W Kenney
Canadian Institute for Health Research (FDN143227)
- Paul W Frankland
National Institutes of Health (P20GM113109)
- Thomas Mueller
Human Frontiers Science Program (RGP0016/2019)
- Thomas Mueller
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: The study was performed in accordance with the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All procedures were approved by the animal care committee of The Hospital for Sick Children (protocol #0000047792).
Reviewing Editor
- Stephen C Ekker, Mayo Clinic, United States
Version history
- Received: May 3, 2021
- Accepted: November 21, 2021
- Accepted Manuscript published: November 22, 2021 (version 1)
- Version of Record published: December 2, 2021 (version 2)
Copyright
© 2021, Kenney et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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A 3D adult zebrafish brain atlas (AZBA) for the digital age
eLife 10:e69988.
https://doi.org/10.7554/eLife.69988
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