The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges

Abstract
Data availability
Article and author information
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Abstract

Nearly 50 different mouse retinal ganglion cell (RGC) types sample the visual scene for distinct features. RGC feature selectivity arises from their synapses with a specific subset of amacrine (AC) and bipolar cell (BC) types, but how RGC dendrites arborize and collect input from these specific subsets remains poorly understood. Here we examine the hypothesis that RGCs employ molecular recognition systems to meet this challenge. By combining calcium imaging and type-specific histological stains we define a family of circuits that express the recognition molecule Sidekick 1 (Sdk1) which include a novel RGC type (S1-RGC) that responds to local edges. Genetic and physiological studies revealed that Sdk1 loss selectively disrupts S1-RGC visual responses which result from a loss of excitatory and inhibitory inputs and selective dendritic deficits on this neuron. We conclude that Sdk1 shapes dendrite growth and wiring to help S1-RGCs become feature selective.

Data availability

Sample registration fields, registration code, and GCaMP6f datasets are available at Dryad (https://doi.org/10.5061/dryad.4xgxd2593).

The following data sets were generated

(2021) Data From: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges
Dryad Digital Repository, doi:10.5061/dryad.4xgxd2593.

https://doi.org/10.5061/dryad.4xgxd2593

The following previously published data sets were used

1. Tran NM*
2. Shekhar K*
3. Whitney IE*
4. Jacobi A*
5. Benhar I
6. Hong G
7. Yan W
8. Adiconis X
9. Arnold ME
10. Lee JM
11. Levin JZ
12. Lin D
13. Wang C
14. Lieber CM
15. Regev A
16. He Z
17. Sanes JR.
(2019) Study: Mouse retinal ganglion cell adult atlas and optic nerve crush time series
Broad Institute Single Cell Portal.

https://singlecell.broadinstitute.org/single_cell/study/SCP509/mouse-retinal-ganglion-cell-adult-atlas-and-optic-nerve-crush-time-series?genes=Sdk1&cluster=Crush%20RGCs&spatialGroups=--&annotation=Cluster--group--cluster&subsample=1000&tab=distribution#study-summary
1. Yan W
2. Laboulaye MA
3. Tran NM
4. Whitney IE
5. Benhar I
6. Sanes JR.
(2020) Study: Mouse Retinal Cell Atlas: Molecular Identification of over Sixty Amacrine Cell Types
Broad Institute Single Cell Portal.

https://singlecell.broadinstitute.org/single_cell/study/SCP919/mouse-retinal-cell-atlas-molecular-identification-of-over-sixty-amacrine-cell-types
1. Shekhar K
2. Lapan SW
3. Whitney IE
4. Tran NM
5. Macosko EZ
6. Kowalczyk M
7. Adiconis X
8. Levin JZ
9. Nemesh J
10. Goldman M
11. McCarroll SA
12. Cepko CL
13. Regev A
14. Sanes JR.
(2016) Study: Retinal Bipolar Neuron Drop-seq
Broad Institute Single Cell Portal.

https://singlecell.broadinstitute.org/single_cell/study/SCP3/retinal-bipolar-neuron-drop-seq

Article and author information

Author details

Pierre-Luc Rochon

McGill University, Montreal, Canada

Competing interests
The authors declare that no competing interests exist.
Catherine Theriault

McGill University, Montreal, Canada

Competing interests
The authors declare that no competing interests exist.
Aline Giselle Rangel Olguin

McGill University, Montreal, Canada

Competing interests
The authors declare that no competing interests exist.
Arjun Krishnaswamy

McGill University, Montreal, Canada

For correspondence
arjun.krishnaswamy@mcgill.ca

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0002-7706-4657

Funding

Canadian Institutes of Health Research (project grant)

Arjun Krishnaswamy

Natural Sciences and Engineering Research Council of Canada (discovery grant)

Arjun Krishnaswamy

Canadian Institutes of Health Research (Graduate Fellowship)

Pierre-Luc Rochon

Fonds de Recherche du Québec - Santé (Graduate Fellowship)

Aline Giselle Rangel Olguin

Consejo Nacional de Ciencia y Tecnología (Graduate Fellowship)

Aline Giselle Rangel Olguin

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: Animals were used in accordance with the rules and regulations established by the Canadian Council on Animal Care and protocol (2017-7889) was approved by the Animal Care Committee at McGill University

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.