GD heatmaps are shown on the left, and FRD heatmaps are shown on the right. Column names for FRD correspond to the reference sample. Organ codes are blood (a), bile (b), right spleen (e), left spleen (f), right kidney (j), left kidney (k), right lung (n), left lung (o), caudate liver (v), right liver (w), left liver (x), and medial liver (y). A clear dichotomy is observed between mice 18 and 19 versus mice 17 and 20. Mice 17 and 20 had substantial replication of bacteria in the bile. This clone spread across the body, indicated by low GD values in multiple organs. FRD values reflect the fact that the bile is a single clone. Starting at the asterisk, rightward movement on the FRD heatmap yields lower nonzero FRD values than downward movement. This indicates that when the non-bile organ is set as the reference, FRD values are low, revealing that only a few barcodes are shared. When the bile is set as the reference, FRD values are much higher, indicating that the shared clones represent a larger fraction of barcodes in the bile. This is quantified in Figure 3C. Given that the Nr of bile is ~1, low GD values are due to essentially a single clone.