Classification and genetic targeting of cell types in the primary taste and premotor center of the adult Drosophila brain

  1. Gabriella R Sterne  Is a corresponding author
  2. Hideo Otsuna
  3. Barry J Dickson
  4. Kristin Scott  Is a corresponding author
  1. University of California Berkeley, United States
  2. Janelia Research Campus, Howard Hughes Medical Institute, United States
  3. University of California, Berkeley, United States

Abstract

Neural circuits carry out complex computations that allow animals to evaluate food, select mates, move toward attractive stimuli, and move away from threats. In insects, the subesophageal zone (SEZ) is a brain region that receives gustatory, pheromonal, and mechanosensory inputs and contributes to the control of diverse behaviors, including feeding, grooming, and locomotion. Despite its importance in sensorimotor transformations, the study of SEZ circuits has been hindered by limited knowledge of the underlying diversity of SEZ neurons. Here, we generate a collection of split-GAL4 lines that provides precise genetic targeting of 138 different SEZ cell types in adult D. melanogaster, comprising approximately one third of all SEZ neurons. We characterize the single cell anatomy of these neurons and find that they cluster by morphology into six supergroups that organize the SEZ into discrete anatomical domains. We find that the majority of local SEZ interneurons are not classically polarized, suggesting rich local processing, whereas SEZ projection neurons tend to be classically polarized, conveying information to a limited number of higher brain regions. This study provides insight into the anatomical organization of the SEZ and generates resources that will facilitate further study of SEZ neurons and their contributions to sensory processing and behavior.

Data availability

Detailed information about the split-GAL4s and available imagery is included in a supplemental database (attached as a supporting file). Image data are publicly available and all lines may be ordered at http://splitgal4.janelia.org.

The following previously published data sets were used

Article and author information

Author details

  1. Gabriella R Sterne

    Molecular & Cell Biology, University of California Berkeley, Berkeley, United States
    For correspondence
    sternegr@berkeley.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-7221-648X
  2. Hideo Otsuna

    Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Barry J Dickson

    Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-0715-892X
  4. Kristin Scott

    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
    For correspondence
    kscott@berkeley.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-3150-7210

Funding

Howard Hughes Medical Institute

  • Gabriella R Sterne
  • Hideo Otsuna
  • Barry J Dickson

National Institute of Diabetes and Digestive and Kidney Diseases (F32DK117671)

  • Gabriella R Sterne

National Institute of General Medical Sciences (R01NS110060)

  • Gabriella R Sterne
  • Kristin Scott

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Ilona C Grunwald Kadow, Technical University of Munich, Germany

Version history

  1. Received: June 26, 2021
  2. Preprint posted: August 8, 2021 (view preprint)
  3. Accepted: September 1, 2021
  4. Accepted Manuscript published: September 2, 2021 (version 1)
  5. Version of Record published: September 16, 2021 (version 2)

Copyright

© 2021, Sterne et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Gabriella R Sterne
  2. Hideo Otsuna
  3. Barry J Dickson
  4. Kristin Scott
(2021)
Classification and genetic targeting of cell types in the primary taste and premotor center of the adult Drosophila brain
eLife 10:e71679.
https://doi.org/10.7554/eLife.71679

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https://doi.org/10.7554/eLife.71679

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