Regulated delivery controls Drosophila Hedgehog, Wingless and Decapentaplegic signaling

  1. Ryo Hatori
  2. Brent M Wood
  3. Guilherme Oliveira Barbosa
  4. Thomas B Kornberg  Is a corresponding author
  1. University of California, San Francisco, United States

Abstract

Morphogen signaling proteins disperse across tissues to activate signal transduction in target cells. We investigated dispersion of Hedgehog (Hh), Wnt homolog Wingless (Wg), and Bone morphogenic protein homolog Decapentaplegic (Dpp) in the Drosophila wing imaginal disc. We discovered that delivery of Hh, Wg, and Dpp to their respective targets is regulated. We found that <5% of Hh and <25% of Wg are taken up by disc cells and activate signaling. The amount of morphogen that is taken up and initiates signaling did not change when the level of morphogen expression was varied between 50-200% (Hh) or 50-350% (Wg). Similar properties were observed for Dpp. We analyzed an area of 150 mm x 150 mm that includes Hh-responding cells of the disc as well as overlying tracheal cells and myoblasts that are also activated by disc-produced Hh. We found that the extent of signaling in the disc was unaffected by the presence or absence of the tracheal and myoblast cells, suggesting that the mechanism that disperses Hh specifies its destinations to particular cells, and that target cells do not take up Hh from a common pool.

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All data generated or analysed during this study are included in the manuscript and supporting files

Article and author information

Author details

  1. Ryo Hatori

    University of California, San Francisco, San Francisco, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Brent M Wood

    University of California, San Francisco, San Francisco, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Guilherme Oliveira Barbosa

    University of California, San Francisco, San Francisco, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-5881-0896
  4. Thomas B Kornberg

    University of California, San Francisco, San Francisco, United States
    For correspondence
    tkornberg@ucsf.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-6879-7066

Funding

National Institute of General Medical Sciences (R35GM122548)

  • Thomas B Kornberg

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Marianne E Bronner, California Institute of Technology, United States

Version history

  1. Received: June 29, 2021
  2. Accepted: July 13, 2021
  3. Accepted Manuscript published: July 22, 2021 (version 1)
  4. Accepted Manuscript updated: July 23, 2021 (version 2)
  5. Version of Record published: August 19, 2021 (version 3)

Copyright

© 2021, Hatori et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Ryo Hatori
  2. Brent M Wood
  3. Guilherme Oliveira Barbosa
  4. Thomas B Kornberg
(2021)
Regulated delivery controls Drosophila Hedgehog, Wingless and Decapentaplegic signaling
eLife 10:e71744.
https://doi.org/10.7554/eLife.71744

Share this article

https://doi.org/10.7554/eLife.71744

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