(A) Representative movement trajectories of wild-type (black), nlp-12(OE) (red), nlp-12(OE);ckr-1(lf) (blue), nlp-12(OE);ckr-2(lf) (orange), and nlp-12(OE);ckr-1(lf);ckr-2(lf) (green) animals during …
Source data for body bending amplitude (Figure 1B).
Source data for frequency of bending angles (Figure 1D).
Source data for in vitro analysis of CKR-1 activation (Figure 1E).
Source data for in vitro analysis of CKR-2 activation (Figure 1F).
(A) Dendrogram (generated using Phylogeny,fr Dereeper et al., 2008). showing the predicted relationship between Drosophila (Dm_CCKLR-1/2), C. elegans (Ce_CKR-1/2), mouse (Mm), and human (Hs) CCK1/2R …
NLP-12–1 and NLP-12–2 elicit Ca2+ responses in cells expressing CKR-1 or CKR-2, but not in cells transfected with an empty pcDNA3.1 vector. Bar graphs indicate the ratio of total Ca2+ response of …
Source data for in vitro controls (ratio of total calcium response).
Schematics showing body bending (A) and head bending (B) angles (solid orange circles indicate the vertices and measured angle in blue) quantified during single worm track analyses of movement (5 …
Source data for body bending measurements during single worm tracking of basal locomotion (Figure 2A).
Source data for head bending measurements during single worm tracking of basal locomotion (Figure 2B).
(A) Schematic of the food search assay indicating the time intervals when reorientations were scored. Wild-type animals increase reorientations during the first 5 min (0–5 min) after removal from …
Source data for reorientations quantified during area restricted search (0–5 min off food, Figure 3C).
Source data for reorientations quantified during dispersal (30–35 min off food, Figure 3E).
Source data for % change in reorientations from mean quantified for DVA photostimulation (Figure 3F).
Frame #s and time points are indicated in each panel. Frame numbers and time points indicated are relative to the first image in each sequence, which represents the start point (frame 0, time 0 s) …
(A) Quantification of reorientations during ARS (0–5 min following removal from food) compare to animals on food. Note that the increased number of forward and reversal coupled reorientations. Bars …
Source data for reorientations quantified on food and during area restricted search (0–5 min off food, Figure 3—figure supplement 2A).
Source data for reorientations quantified during area restricted search (0–5 min off food, Figure 3—figure supplement 2B).
(A) Quantification of reorientations during ARS (0–5 min following removal from food) for the genotypes indicated. Rescue refers to transgenic expression of wild-type ckr-1 or ckr-2 in ckr-1(ok2502);…
Source data for reorientations quantified during area restricted search (0–5 min off food, Figure 3—figure supplement 3A).
Source data for reorientations quantified during area restricted search (0–5 min off food, Figure 3—figure supplement 3B).
Source data for reorientations quantified during area restricted search (0–5 min off food, Figure 3—figure supplement 3C).
(A) Representative movement trajectories of wild-type (black), ckr-1(OE) (blue) and ckr-1(OE); nlp-12(lf) (green) animals for 30 s on NGM agar plates seeded with OP50 bacteria. ckr-1(OE) refers to …
Source data for frequency of bending angles (Figure 4B).
Source data for body bending amplitude (Figure 4C).
(A) Confocal maximum intensity projection of adult expressing the Pckr-1::ckr-1::SL2::GFP reporter. Note that the expression in multiple head neurons (white box) and a subset of ventral nerve cord …
Source data for body bending amplitude (Figure 5C).
(A) Confocal maximum intensity projections of a segment of the ventral nerve cord of a transgenic animal co-expressing Pckr-1::ckr-1::SL2::GFP and the cholinergic reporter Pacr-2::mCherry. ckr-1 is …
Confocal maximum intensity projections of transgenic worm expressing Pckr-1::ckr-1::SL2::mCherry and Pckr-2::GFP. (A) ckr-1 and ckr-2 expression in the entire worm. Both ckr-1 and ckr-2 are highly …
(A) Representative tracks (1 min) for indicated genotypes. Asterisks indicate the position of animal at the beginning of recordings. Note that the increased reorientations and body bending depth in …
Source data for frequency of bending angles (Figure 6B).
Source data for frequency of bending angles (Figure 6E).
Source data for bending amplitude (Figure 6F).
(A) Representative tracks (30 s) for transgenic animals with high levels of cell-specific ckr-1 overexpression (Pflp-22∆4::ckr-1) in wild-type (top) or nlp-12 deletion background (bottom). Asterisks …
Source data for frequency of bending angles (Figure 6—figure supplement 1B).
Source data for frequency of bending angles (Figure 6—figure supplement 1C).
Total reorientations measured during 0–5 min following removal from food for the genotypes indicated. ckr-1 rescue refers to expression of wild-type ckr-1 (5 ng/µl) in ckr-1(ok2502) animals using …
Source data for reorientations quantified during area restricted search (0–5 min off food, Figure 7A).
Source data for reorientations quantified during SMD photostimulation (Figure 7C).
Source data for reorientations quantified during area restricted search upon SMD silencing (0–5 min off food, Figure 7D).
(A) Average body bending angle distribution (mean ± SEM) plotted for wild-type control animals (soid black circles, n=8) and Pflp-22∆4::ckr-1 (solid orange squares, n=8). Low level (5 ng/µl) …
Source data for frequency of bending angles (Figure 7—figure supplement 1A).
Source data for body bending amplitude quantified during SMD photostimulation (Figure 7—figure supplement 1B).
(A–C) Representative heat maps showing activity of SMD neurons in transgenic animals (Pflp-22∆4::GCaMP6s::SL2::mCherry) during ARS (A) and dispersal (B) for wild type, and ARS for ckr-1(ok2502) (C). …
Source data for GCaMP6s/mCherry ratio during SMD calcium imaging (Figure 8A–D).
Source data for mean GCaMP6s/mCherry ratio during SMD calcium imaging (Figure 8E).
Corresponding behaviors are annotated by shading as indicated.
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Stains generated/used in this work.
Identification (method of ID, marker and strain indicated for each neuron) to determine ckr-1 expressing neurons.
* Indicated strains were crossed into ufIs141 (Pckr-1::ckr-1::SL2::GFP) to generate strains to determine colocalization. #+ or – indicates presence or absence of ckr-1 expression in identified neuron. * Indicated strains were crossed into ufIs141 to generate strains to determine colocalization, #+ indicates ckr-1 expression, - indicates absence.
Promoters used in ckr-1(OE) screen (Figure 5C) indicating expression pattern.
**Bold indicates neurons where ckr-1 is expressed.
Plasmid constructs used in cell specific ckr-1(OE) screen or cell-specific rescue (Figures 5C and 7A).
For cell specific overexpression or rescue of ckr-1, ckr-1 minigene was expressed under indicated promoters. Entry vectors containing promoters recombined with destination vectors pRB12 or pRB13 for cell-specific overexpression or rescue of ckr-1.
Promoter lengths and primer information for promoters used.