Strain, strain background (Mus musculus) | C57BL/6 J | The Jackson Laboratory | RRID:IMSR_JAX:000664 | |
Strain, strain background (M. musculus) | B6.Cg-Commd10Tg(Vav1-icre)A2Kio/J | The Jackson Laboratory | RRID:IMSR_JAX:008610 | |
Strain, strain background (M. musculus) | B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ | The Jackson Laboratory | RRID:IMSR_JAX:022071 | |
Strain, strain background (M. musculus) | B6NTac.Cg-Tg(Lck-cre)1Cwi/Mmnc | Lee et al., 2001 | RRID:MMRRC_037396-UNC | |
Strain, strain background (M. musculus) | Cicfloxed | Lu et al., 2017; Park et al., 2017 | RRID:IMSR_JAX:030555 | |
Strain, strain background (M. musculus) | CicFLAG/FLAG | Park et al., 2019 | PMID:30810242 | |
Strain, strain background (M. musculus) | B6.Cg-Tg(TcraH-Y,TcrbH-Y)71Vbo | Kisielow et al., 1988 | RRID:MGI:3588781 | |
Strain, strain background (M. musculus) | B6.Cg-Tg(TcraTcrb)425Cbn/J | The Jackson Laboratory | RRID:IMSR_JAX:004194 | |
Strain, strain background (M. musculus) | B6.Cg-Foxp3tm2Tch/J | The Jackson Laboratory | RRID:IMSR_JAX:006772 | |
Strain, strain background (M. musculus) | Spry4-/- | This paper | N/A | generated using Spry4tm1a(KOMP)Mbp embryonic stem cells obtained from the UC Davis KOMP repository. |
Cell line (Homo-sapiens) | Platinum-E (Plat-E) Retroviral Packaging Cell Line | Cell Biolabs | Cat# RV-101, RRID:CVCL_B488 | |
Antibody | Anti-mouse CD3ε (Armenian Hamster monoclonal) | BioLegendTonbo Biosciences | Cat# 100329, RRID:AB_1877171Cat# 50–0031, RRID:AB_2621730 | FC (1:300) |
Antibody | Anti-mouse CD4 (Rat monoclonal) | BiolegendBD Biosciences | Cat# 100552, RRID:AB_2563053Cat# 562891, RRID:AB_2737870 | FC (1:300) |
Antibody | Anti-mouse CD5 (Rat monoclonal) | Biolegend eBioscience | Cat# 100625, RRID:AB_2563928Cat# 45-0051-80, RRID:AB_914332 | FC (1:300) |
Antibody | Anti-mouse CD8α (Rat monoclonal) | BioLegend | Cat# 100723, RRID:AB_389304Cat# 100721, RRID:AB_312760 | FC (1:300) |
Antibody | Anti-mouse CD11b (Rat monoclonal) | BioLegend | Cat# 101211, RRID:AB_312794 | FC (1:300) |
Antibody | Anti-mouse CD11c (Armenian Hamster monoclonal) | BioLegend | Cat# 117309, RRID:AB_313778 | FC (1:300) |
Antibody | Anti-mouse CD19 (Rat monoclonal) | BD Biosciences | Cat# 561738, RRID:AB_10893995 | FC (1:300) |
Antibody | Anti-mouse CD24 (Rat monoclonal) | BioLegend | Cat# 101819, RRID:AB_572010 | FC (1:300) |
Antibody | Anti-mouse CD25 (Rat monoclonal) | Tonbo Biosciences | Cat# 75–0251, RRID:AB_2621943 | FC (1:300) |
Antibody | Anti-mouse CD44 (Rat monoclonal) | BD Biosciences | Cat# 553133, RRID:AB_2076224Cat# 561860, RRID:AB_10895375 | FC (1:300) |
Antibody | Anti-mouse CD62L (Rat monoclonal) | BD Biosciences | Cat# 560516, RRID:AB_1645257 | FC (1:300) |
Antibody | Anti-mouse CD69 (Armenian Hamster monoclonal) | BioLegend | Cat# 104513, RRID:AB_492844Cat# 104545, RRID:AB_2686969 | FC (1:300) |
Antibody | Anti-mouse CD73 (Rat monoclonal) | Biolegend eBioscience | Cat# 127223, RRID:AB_2716102Cat# 12-0731-81, RRID:AB_763516 | FC (1:300) |
Antibody | Anti-mouse CD90.1 (Mouse monoclonal) | eBioscience | Cat# 45-0900-82, RRID:AB_2573662 | FC (1:300) |
Antibody | Anti-mouse CD90.2 (Rat monoclonal) | BioLegend | Cat# 140303, RRID:AB_10642686 | FC (1:300) |
Antibody | Anti-mouse CXCR5 (Rat monoclonal) | BD Biosciences | Cat# 551960, RRID:AB_394301 | FC (1:100) |
Antibody | Anti-mouse TCRβ (Armenian Hamster monoclonal) | Tonbo Biosciences | Cat# 35–5961, RRID:AB_2621723 | FC (1:300) |
Antibody | Anti-mouse TCR Vβ2 (Rat monoclonal) | BioLegend | Cat# 127908, RRID:AB_1227784 | FC (1:300) |
Antibody | Anti-mouse TCR Vβ5.1/5.2 (Mouse monoclonal) | BD Biosciences | Cat# 553189, RRID:AB_394697 | FC (1:300) |
Antibody | Anti-mouse TCR Vβ6 (Rat monoclonal) | BioLegend | Cat# 140004, RRID:AB_10643583 | FC (1:300) |
Antibody | Anti-mouse TCR Vβ7 (Rat monoclonal) | BioLegend | Cat# 118308, RRID:AB_893628 | FC (1:300) |
Antibody | Anti-mouse TCR Vβ8.1/8.2 (Rat monoclonal) | BioLegend | Cat# 118408, RRID:AB_1134109 | FC (1:300) |
Antibody | Anti-mouse TCR Vβ8.3 (Armenian Hamster monoclonal) | BD Biosciences | Cat# 553664, RRID:AB_394980 | FC (1:300) |
Antibody | Anti-mouse TCR Vβ9 (Mouse monoclonal) | BioLegend | Cat# 139804, RRID:AB_10641563 | FC (1:300) |
Antibody | Anti-mouse TCR Vβ11 (Rat monoclonal) | BioLegend | Cat# 125907, RRID:AB_1227781 | FC (1:300) |
Antibody | Anti-mouse TCR Vβ12 (Mouse monoclonal) | BioLegend | Cat# 139704, RRID:AB_10639729 | FC (1:300) |
Antibody | Anti-mouse TCR Vβ13 (Mouse monoclonal) | BioLegend | Cat# 140704, RRID:AB_10639945 | FC (1:300) |
Antibody | Anti-mouse TCRγ/δ (Armenian Hamster monoclonal) | eBioscience | Cat# 17-5711-81, RRID:AB_842757 | FC (1:300) |
Antibody | Anti-mouse NK-1.1 (Mouse monoclonal) | BioLegend | Cat# 108709, RRID:AB_313396 | FC (1:300) |
Antibody | Anti-mouse TER-119 (Rat monoclonal) | eBioscience | Cat# 17-5921-81, RRID:AB_469472 | FC (1:300) |
Antibody | Anti-mouse Gr-1 (Rat monoclonal) | eBioscience | Cat# 17-5931-81, RRID:AB_469475 | FC (1:300) |
Antibody | Anti-mouse TCR H-Y (Mouse monoclonal) | eBioscience | Cat# 11-9930-81, RRID:AB_465452 | FC (1:300) |
Antibody | Anti-mouse PD-1 (Rat monoclonal) | eBioscience | Cat# 11-9981-81, RRID:AB_465466 | FC (1:300) |
Antibody | Anti-mouse Nur77 (Mouse monoclonal) | eBioscience | Cat# 12-5965-82, RRID:AB_1257209 | FC (1:100) |
Antibody | Anti-T-bet (Mouse monoclonal) | eBioscience | Cat# 12-5825-80, RRID:AB_925762 | FC (1:100) |
Antibody | Anti-RORγt (Rat monoclonal) | eBioscience | Cat# 12-5825-80, RRID:AB_925762 | FC (1:100) |
Antibody | Anti-Foxp3 (Rat monoclonal) | eBioscience | Cat# 17-5773-80, RRID:AB_469456 | FC (1:100) |
Antibody | Anti-DYKDDDDK(flag) Tag antibody (Rat monoclonal) | BioLegend | Cat# 637309, RRID:AB_2563147 | FC (1:100) |
Antibody | PE Donkey anti-rabbit IgG (min. x-reactivity) antibody (Donkey Polyclonal) | BioLegend | Cat# 406421, RRID:AB_2563484 | FC (1:100) |
Antibody | Anti-CIC (Rabbit polyclonal) | Kim et al., 2015 | PMID:25653040 | WB (1:1000) |
Antibody | PLCγ1 (D9H10) XP Rabbit mAb antibody (Rabbit monoclonal) | Cell Signaling Technology | Cat# 5690, RRID:AB_10691383 | WB (1:1000) |
Antibody | Phospho-PLC 1 (Tyr783) antibody (Rabbit polyclonal) | Cell Signaling Technology | Cat# 2821, RRID:AB_330855 | WB (1:500) |
Antibody | Zap-70 (99F2) Rabbit mAb antibody (Rabbit monoclonal) | Cell Signaling Technology | Cat# 2705, RRID:AB_2273231 | WB (1:1000) |
Antibody | Phospho-Zap-70 (Tyr319)/Syk (Tyr352) antibody (Rabbit polyclonal) | Cell Signaling Technology | Cat# 2701, RRID:AB_331600 | WB (1:500) |
Antibody | SAPK/JNK antibody (Rabbit polyclonal) | Cell Signaling Technology | Cat# 9252, RRID:AB_2250373 | WB (1:2000) |
Antibody | Phospho-SAPK/JNK (Thr183/Tyr185) antibody (Rabbit polyclonal) | Cell Signaling Technology | Cat# 9251, RRID:AB_331659 | WB (1:1000) |
Antibody | p44/42 MAPK (Erk1/2) antibody (Rabbit polyclonal) | Cell Signaling Technology | Cat# 9102, RRID:AB_330744 | WB (1:2000) |
Antibody | Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Rabbit monoclonal) | Cell Signaling Technology | Cat# 4370, RRID:AB_2315112 | WB (1:1000)FC (1:100) |
Antibody | Anti-p38 MAPK antibody (Rabbit polyclonal) | Cell Signaling Technology | Cat# 9212, RRID:AB_330713 | WB (1:2000) |
Antibody | Phospho-p38 MAPK (Thr180/ Tyr182) antibody (Rabbit polyclonal) | Cell Signaling Technology | Cat# 9211, RRID:AB_331641 | WB (1:1000) |
Antibody | β-Actin Antibody (Mouse monoclonal) | Santa Cruz Biotechnology | Cat# sc-47778, RRID:AB_626632 | WB (1:1000) |
Antibody | α-Tubulin antibody (A-6) (Mouse monoclonal) | Santa Cruz Biotechnology | Cat# sc-398103, RRID:AB_2832217 | WB (1:1000) |
Antibody | Purified NA/LE Hamster Anti-Mouse CD3e (Armenian Hamster monoclonal) | BD Biosciences | Cat# 553057, RRID:AB_394590 | (10 µg/mL) |
Antibody | AffiniPure Goat Anti-Armenian Hamster IgG (H + L) (Goat polyclonal) | Jackson ImmunoResearch Labs | Cat# 127-005-160, RRID:AB_2338972 | (25 µg/mL) |
Recombinant DNA reagent | pMIGR1-GFP (plasmid) | Pear et al., 1998 | RRID:Addgene_27490 | |
Recombinant DNA reagent | pMIGR1-SPRY4-GFP (plasmid) | This paper | N/A | CDS of mouse Spry4 was inserted into pMIGR1-GFP. |
Recombinant DNA reagent | pMIGR1-DUSP6-GFP (plasmid) | This paper | N/A | CDS of mouse Dusp6 was inserted into pMIGR1-GFP. |
Recombinant DNA reagent | pCL-Eco (plasmid) | Naviaux et al., 1996 | RRID:Addgene_12371 | Retrovirus packaging vector |
Sequence-based reagent | primers used in qRT-PCR | This paper | | See Supplementary file 3 for sequence information. |
Peptide, recombinant protein | Streptavidin | Southern Biotech | Cat# 7100–01 | |
Peptide, recombinant protein | Streptavidin | eBioscienceBD Biosciences | Cat# 45-4317-80, RRID:AB_10260035Cat# 554067, RRID:AB_10050396 | FC (1:100) |
Commercial assay or kit | Fixable Viability Dye eFluor 780 | eBioscience | Cat# 65-0865-14 | |
Commercial assay or kit | Ghost Dye Violet 510 | Tonbo Biosciences | Cat# 13–0870 | |
Commercial assay or kit | Foxp3/ Transcription Factor Staining Buffer Set | eBioscience | Cat# 00-5523-00 | |
Commercial assay or kit | BD Cytofix Fixation Buffer | BD Biosciences | Cat# 554655, RRID:AB_2869005 | |
Commercial assay or kit | FuGENE HD Transfection Reagent | Promega | Cat# E2311 | |
Commercial assay or kit | RiboEx | GeneAll | Cat# 301–002 | |
Commercial assay or kit | GoScript Reverse Transcriptase Kit | Promega | Cat# A5001 | |
Commercial assay or kit | SYBR Green Realtime PCR Master Mix | TOYOBO | Cat# TOQPK-201 | |
Commercial assay or kit | EasySep Mouse Streptavidin RapidSpheres Isolation Kit | Stem Cell Technologies | Cat# 19,860 | |
Commercial assay or kit | BCA Protein Assay Kit | Pierce | Cat# 23,225 | |
Commercial assay or kit | Clarity Western ECL Substrate | Bio-Rad | Cat# 1705061 | |
Commercial assay or kit | SuperSignal West Dura Extended Duration Substrate | Thermo Scientific | Cat# 34,076 | |
Chemical compound, drug | Indo-1, AM, cell permeant | Invitrogen | Cat# I1203 | |
Chemical compound, drug | Ionomycin from Streptomyces conglobatus | Sigma-Aldrich | Cat# I9657-1MG | |
Chemical compound, drug | Hexadimethrine bromide | Sigma-Aldrich | Cat# H9268-10G | |
Software, algorithm | FlowJo | Tree Star Inc. | RRID:SCR_008520, https://www.flowjo.com/solutions/flowjo | |
Software, algorithm | ImageJ | NIH | RRID:SCR_003070, https://imagej.nih.gov/ij/ | |
Software, algorithm | GraphPad Prism 7 | GraphPad Software | RRID:SCR_002798, https://www.graphpad.com/scientific- software/prism/ | |