1. Immunology and Inflammation

Regulation of positive and negative selection and TCR signaling during thymic T cell development by capicua

  1. Soeun Kim
  2. Guk-Yeol Park
  3. Jong Seok Park
  4. Jiho Park
  5. Hyebeen Hong
  6. Yoontae Lee  Is a corresponding author
  1. Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Republic of Korea
  2. Institute of Convergence Science, Yonsei University, Republic of Korea
https://doi.org/10.7554/eLife.71769
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Cite this article
  1. Soeun Kim
  2. Guk-Yeol Park
  3. Jong Seok Park
  4. Jiho Park
  5. Hyebeen Hong
  6. Yoontae Lee
(2021)
Regulation of positive and negative selection and TCR signaling during thymic T cell development by capicua
eLife 10:e71769.
https://doi.org/10.7554/eLife.71769
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10 figures, 1 table and 5 additional files

Figures

Figure 1 with 3 supplements
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Altered T cell development in Cicf/f;Vav1-Cre mice.

(A) Capicua (CIC) protein levels in thymic T cell subsets. Thymocytes of CicFLAG/FLAG mice (N = 7) were subjected to flow cytometry using anti-FLAG antibody. Representative histograms of CIC-FLAG …

Figure 1—source data 1

Raw data for Figure 1.

https://cdn.elifesciences.org/articles/71769/elife-71769-fig1-data1-v2.xlsx
Figure 1—figure supplement 1
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Analysis of thymic γδT cells in Cicf/f;Vav1-Cre mice.

(A) Flow cytometric analysis of γδT cells in the thymus of 7-week-old Cicf/f and Cicf/f;Vav1-Cre mice. Representative plots (left) and the frequency of total γδT cells (right) are shown. GL3: γδTCR. …

Figure 1—figure supplement 2
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Analysis of splenic and thymic T cell subsets in 1-week-old Cicf/f;Vav1-Cre mice.

(A) Flow cytometric analysis of splenocytes from 1-week-old Cicf/f and Cicf/f;Vav1-Cre mice. Representative plots of CD4 and CD8 expression in splenocytes, frequency and number of CD4+ and CD8+ T …

Figure 1—figure supplement 3
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Flow cytometric analysis of recirculating CD4+ T cells in the thymus of Cicf/f;Vav1-Cre mice.

(A and B) Thymocytes from (A) 1-week-old Cicf/f (N = 4) and Cicf/f;Vav1-Cre (N = 5) and (B) 9-week-old Cicf/f (N = 8) and Cicf/f;Vav1-Cre (N = 5) mice were analyzed for recirculating CD4+ …

Figure 2
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Stable capicua (CIC) expression in double-positive (DP) cells of Cicf/f;Cd4-Cre mice.

(A) Western blotting for detection of CIC levels in double-negative 1–2 (DN1-2), DN3, DN4, immature CD8+ single-positive (ISP), double-positive (DP), CD4+ SP, and CD8+ SP cells from Cicf/f, Cicf/f;Cd…

Figure 2—source data 1

Original and labelled files for western blot and PCR gel images.

https://cdn.elifesciences.org/articles/71769/elife-71769-fig2-data1-v2.zip
Figure 3 with 1 supplement
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Defective positive selection in the absence of capicua (CIC).
Figure 3—source data 1

Raw data for Figure 3.

https://cdn.elifesciences.org/articles/71769/elife-71769-fig3-data1-v2.xlsx
Figure 3—figure supplement 1
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Analysis of H-Y TCR transgenic CD8+ T and immature CD8+ single positive (ISP) cells in female H-Y mice.

(A) Flow cytometric analysis of thymocytes from female H-Y;Cicf/f (N = 3) and H-Y;Cicf/f;Vav1-Cre (N = 4) mice for H-Y TCR (T3.70) and CD8 expression. Frequencies of H-Y TCR+ CD8+ cells among total …

Figure 4 with 2 supplements
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Defective negative selection in the absence of capicua (CIC).

(A) qRT-PCR quantification of Bcl2 expression in double-negative (DN), double-positive (DP), CD4+ single-positive (4SP), and CD8+ SP (8SP, TCRβhi) cells of Cicf/f and Cicf/f;Vav1-Cre mice. N = 3 for …

Figure 4—source data 1

Raw data for Figure 4.

https://cdn.elifesciences.org/articles/71769/elife-71769-fig4-data1-v2.xlsx
Figure 4—figure supplement 1
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Analysis of H-Y TCR transgenic CD8+ T and immature CD8+ single positive (ISP) cells in male H-Y mice.

(A) Flow cytometric analysis of thymocytes from male H-Y;Cicf/f (N = 3) and H-Y;Cicf/f;Vav1-Cre (N = 6) mice for H-Y TCR (T3.70) and CD8 expression. Frequencies of H-Y TCR+ CD8+ cells among total …

Figure 4—figure supplement 2
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Analysis of TCR Vβ chains in CIC-deficient CD4+ SP thymocytes.

(A and B) Thymocytes from + –9 week-old Cicf/f and Cicf/f;Vav1-Cre mice were analyzed for the expression of 10 different types of TCR Vβ chains by flow cytometry. (A) CD4+CD25-Foxp3- non-Treg and (B)…

Figure 5 with 1 supplement
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Attenuated TCR signaling in capicua (CIC)-deficient double-positive (DP) thymocytes.

(A) Thymocytes from 7-week-old Cicf/f, Cicf/f;Cd4-Cre, and Cicf/f;Vav1-Cre mice were FACS-gated into the DP, CD4+ single-positive (SP), and CD8+ SP (TCRβhi) cell population, and the CD5 mean …

Figure 5—source data 1

Raw data for Figure 5.

https://cdn.elifesciences.org/articles/71769/elife-71769-fig5-data1-v2.xlsx
Figure 5—source data 2

Original and labeled files for western blot images.

https://cdn.elifesciences.org/articles/71769/elife-71769-fig5-data2-v2.zip
Figure 5—figure supplement 1
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Analysis of CD5 expression in TCR transgenic thymocytes.

Thymocytes from (A–C) female H-Y;Cicf/f (N = 3) and H-Y;Cicf/f;Vav1-Cre (N = 4) mice and (D–F) OT-II;Cicf/f (N = 5) and OT-II;Cicf/f;Vav1-Cre (N = 5) mice were analyzed for CD69 and CD5 expression. …

Figure 6 with 1 supplement
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Altered T cell development and TCR intensity in Cicf/f;Vav1-Cre mice are caused by CIC loss in T cells.

(A) Schematic of the generation and analysis of mixed bone marrow (BM) chimeric mice. Equal numbers of BM cells from Cicf/f;Thy1.1/1.2 (WT) and Cicf/f;Vav1-Cre;Thy1.1/1.1 (KO) mice were mixed and …

Figure 6—source data 1

Raw data for Figure 6.

https://cdn.elifesciences.org/articles/71769/elife-71769-fig6-data1-v2.xlsx
Figure 6—figure supplement 1
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Analysis of thymocytes in Cicf/f;pLck-Cre mice.
Figure 7 with 1 supplement
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Identification of capicua (CIC) target genes regulating TCR signaling in double-positive (DP) thymocytes.

(A) Volcano plot showing differentially expressed genes (DEGs) in CIC-deficient DP thymocytes (fold change, > 2; adjusted P value, < 0.05). CIC target genes and Bcl2 are indicated at the …

Figure 7—source data 1

Raw data for Figure 7.

https://cdn.elifesciences.org/articles/71769/elife-71769-fig7-data1-v2.xlsx
Figure 7—figure supplement 1
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Analysis of T cell subsets in the thymus and spleen of Spry4-/-;Cicf/f;Vav1-Cre mice.

(A) Flow cytometric analysis of thymocytes from 7-week-old Cicf/f (N = 4), Cicf/f;Vav1-Cre (N = 6), and Spry4-/-;Cicf/f;Vav1-Cre (N = 4) mice for the frequency of DN1–four subsets. (B) FACS analysis …

Author response image 1
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Histograms of Nur77 expression in double-positive (DP) thymocytes (A) Flow cytometric analysis of Nur77 expression in DP thymocytes.

Various concentrations of freshly isolated thymocytes were incubated with plate-coated anti-CD3 (5 μg/ml) and anti-CD28 (10 μg/ml) antibodies for 2 h, followed by staining of surface markers (CD4, …

Author response image 2
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Efficiency of retroviral transduction of thymocytes.

FACS plots showing the frequency of GFP+ thymocytes transduced with retrovirus co-expressing GFP and control, SPRY4, or DUSP6. Three independent experiments were performed.

Author response image 3
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Comparison of the extent of preserved GFP signal after cell permeabilization by different methods.

(A) Flow cytometric analysis of GFP expression in live thymocytes infected with GFP-expressing retrovirus. Representative FACS plots are presented. No perm: without permeabilization, eBioscience: …

Tables

Key resources table
Reagent type (species) or resourceDesignationSource or referenceIdentifiersAdditional information
Strain, strain background (Mus musculus)C57BL/6 JThe Jackson LaboratoryRRID:IMSR_JAX:000664
Strain, strain background (M. musculus)B6.Cg-Commd10Tg(Vav1-icre)A2Kio/JThe Jackson LaboratoryRRID:IMSR_JAX:008610
Strain, strain background (M. musculus)B6.Cg-Tg(Cd4-cre)1Cwi/BfluJThe Jackson LaboratoryRRID:IMSR_JAX:022071
Strain, strain background (M. musculus)B6NTac.Cg-Tg(Lck-cre)1Cwi/MmncLee et al., 2001RRID:MMRRC_037396-UNC
Strain, strain background (M. musculus)CicfloxedLu et al., 2017; Park et al., 2017RRID:IMSR_JAX:030555
Strain, strain background (M. musculus)CicFLAG/FLAGPark et al., 2019PMID:30810242
Strain, strain background (M. musculus)B6.Cg-Tg(TcraH-Y,TcrbH-Y)71VboKisielow et al., 1988RRID:MGI:3588781
Strain, strain background (M. musculus)B6.Cg-Tg(TcraTcrb)425Cbn/JThe Jackson LaboratoryRRID:IMSR_JAX:004194
Strain, strain background (M. musculus)B6.Cg-Foxp3tm2Tch/JThe Jackson LaboratoryRRID:IMSR_JAX:006772
Strain, strain background (M. musculus)Spry4-/-This paperN/Agenerated using Spry4tm1a(KOMP)Mbp embryonic stem cells obtained from the UC Davis KOMP repository.
Cell line (Homo-sapiens)Platinum-E (Plat-E) Retroviral Packaging Cell LineCell BiolabsCat# RV-101, RRID:CVCL_B488
AntibodyAnti-mouse CD3ε (Armenian Hamster monoclonal)BioLegendTonbo BiosciencesCat# 100329, RRID:AB_1877171Cat# 50–0031, RRID:AB_2621730FC (1:300)
AntibodyAnti-mouse CD4 (Rat monoclonal)BiolegendBD BiosciencesCat# 100552, RRID:AB_2563053Cat# 562891, RRID:AB_2737870FC (1:300)
AntibodyAnti-mouse CD5 (Rat monoclonal)Biolegend eBioscienceCat# 100625, RRID:AB_2563928Cat# 45-0051-80, RRID:AB_914332FC (1:300)
AntibodyAnti-mouse CD8α (Rat monoclonal)BioLegendCat# 100723, RRID:AB_389304Cat# 100721, RRID:AB_312760FC (1:300)
AntibodyAnti-mouse CD11b (Rat monoclonal)BioLegendCat# 101211, RRID:AB_312794FC (1:300)
AntibodyAnti-mouse CD11c (Armenian Hamster monoclonal)BioLegendCat# 117309, RRID:AB_313778FC (1:300)
AntibodyAnti-mouse CD19 (Rat monoclonal)BD BiosciencesCat# 561738, RRID:AB_10893995FC (1:300)
AntibodyAnti-mouse CD24 (Rat monoclonal)BioLegendCat# 101819, RRID:AB_572010FC (1:300)
AntibodyAnti-mouse CD25 (Rat monoclonal)Tonbo BiosciencesCat# 75–0251, RRID:AB_2621943FC (1:300)
AntibodyAnti-mouse CD44 (Rat monoclonal)BD BiosciencesCat# 553133, RRID:AB_2076224Cat# 561860, RRID:AB_10895375FC (1:300)
AntibodyAnti-mouse CD62L (Rat monoclonal)BD BiosciencesCat# 560516, RRID:AB_1645257FC (1:300)
AntibodyAnti-mouse CD69 (Armenian Hamster monoclonal)BioLegendCat# 104513, RRID:AB_492844Cat# 104545, RRID:AB_2686969FC (1:300)
AntibodyAnti-mouse CD73 (Rat monoclonal)Biolegend eBioscienceCat# 127223, RRID:AB_2716102Cat# 12-0731-81, RRID:AB_763516FC (1:300)
AntibodyAnti-mouse CD90.1 (Mouse monoclonal)eBioscienceCat# 45-0900-82, RRID:AB_2573662FC (1:300)
AntibodyAnti-mouse CD90.2 (Rat monoclonal)BioLegendCat# 140303, RRID:AB_10642686FC (1:300)
AntibodyAnti-mouse CXCR5 (Rat monoclonal)BD BiosciencesCat# 551960, RRID:AB_394301FC (1:100)
AntibodyAnti-mouse TCRβ (Armenian Hamster monoclonal)Tonbo BiosciencesCat# 35–5961, RRID:AB_2621723FC (1:300)
AntibodyAnti-mouse TCR Vβ2 (Rat monoclonal)BioLegendCat# 127908, RRID:AB_1227784FC (1:300)
AntibodyAnti-mouse TCR Vβ5.1/5.2 (Mouse monoclonal)BD BiosciencesCat# 553189, RRID:AB_394697FC (1:300)
AntibodyAnti-mouse TCR Vβ6 (Rat monoclonal)BioLegendCat# 140004, RRID:AB_10643583FC (1:300)
AntibodyAnti-mouse TCR Vβ7 (Rat monoclonal)BioLegendCat# 118308, RRID:AB_893628FC (1:300)
AntibodyAnti-mouse TCR Vβ8.1/8.2 (Rat monoclonal)BioLegendCat# 118408, RRID:AB_1134109FC (1:300)
AntibodyAnti-mouse TCR Vβ8.3 (Armenian Hamster monoclonal)BD BiosciencesCat# 553664, RRID:AB_394980FC (1:300)
AntibodyAnti-mouse TCR Vβ9 (Mouse monoclonal)BioLegendCat# 139804, RRID:AB_10641563FC (1:300)
AntibodyAnti-mouse TCR Vβ11 (Rat monoclonal)BioLegendCat# 125907, RRID:AB_1227781FC (1:300)
AntibodyAnti-mouse TCR Vβ12 (Mouse monoclonal)BioLegendCat# 139704, RRID:AB_10639729FC (1:300)
AntibodyAnti-mouse TCR Vβ13 (Mouse monoclonal)BioLegendCat# 140704, RRID:AB_10639945FC (1:300)
AntibodyAnti-mouse TCRγ/δ (Armenian Hamster monoclonal)eBioscienceCat# 17-5711-81, RRID:AB_842757FC (1:300)
AntibodyAnti-mouse NK-1.1 (Mouse monoclonal)BioLegendCat# 108709, RRID:AB_313396FC (1:300)
AntibodyAnti-mouse TER-119 (Rat monoclonal)eBioscienceCat# 17-5921-81, RRID:AB_469472FC (1:300)
AntibodyAnti-mouse Gr-1 (Rat monoclonal)eBioscienceCat# 17-5931-81, RRID:AB_469475FC (1:300)
AntibodyAnti-mouse TCR H-Y (Mouse monoclonal)eBioscienceCat# 11-9930-81, RRID:AB_465452FC (1:300)
AntibodyAnti-mouse PD-1 (Rat monoclonal)eBioscienceCat# 11-9981-81, RRID:AB_465466FC (1:300)
AntibodyAnti-mouse Nur77 (Mouse monoclonal)eBioscienceCat# 12-5965-82, RRID:AB_1257209FC (1:100)
AntibodyAnti-T-bet (Mouse monoclonal)eBioscienceCat# 12-5825-80, RRID:AB_925762FC (1:100)
AntibodyAnti-RORγt (Rat monoclonal)eBioscienceCat# 12-5825-80, RRID:AB_925762FC (1:100)
AntibodyAnti-Foxp3 (Rat monoclonal)eBioscienceCat# 17-5773-80, RRID:AB_469456FC (1:100)
AntibodyAnti-DYKDDDDK(flag) Tag antibody (Rat monoclonal)BioLegendCat# 637309, RRID:AB_2563147FC (1:100)
AntibodyPE Donkey anti-rabbit IgG (min. x-reactivity) antibody (Donkey Polyclonal)BioLegendCat# 406421, RRID:AB_2563484FC (1:100)
AntibodyAnti-CIC (Rabbit polyclonal)Kim et al., 2015PMID:25653040WB (1:1000)
AntibodyPLCγ1 (D9H10) XP Rabbit mAb antibody (Rabbit monoclonal)Cell Signaling TechnologyCat# 5690, RRID:AB_10691383WB (1:1000)
AntibodyPhospho-PLC 1 (Tyr783) antibody (Rabbit polyclonal)Cell Signaling TechnologyCat# 2821, RRID:AB_330855WB (1:500)
AntibodyZap-70 (99F2) Rabbit mAb antibody (Rabbit monoclonal)Cell Signaling TechnologyCat# 2705, RRID:AB_2273231WB (1:1000)
AntibodyPhospho-Zap-70 (Tyr319)/Syk (Tyr352) antibody (Rabbit polyclonal)Cell Signaling TechnologyCat# 2701, RRID:AB_331600WB (1:500)
AntibodySAPK/JNK antibody (Rabbit polyclonal)Cell Signaling TechnologyCat# 9252, RRID:AB_2250373WB (1:2000)
AntibodyPhospho-SAPK/JNK (Thr183/Tyr185) antibody (Rabbit polyclonal)Cell Signaling TechnologyCat# 9251, RRID:AB_331659WB (1:1000)
Antibodyp44/42 MAPK (Erk1/2) antibody (Rabbit polyclonal)Cell Signaling TechnologyCat# 9102, RRID:AB_330744WB (1:2000)
AntibodyPhospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Rabbit monoclonal)Cell Signaling TechnologyCat# 4370, RRID:AB_2315112WB (1:1000)FC (1:100)
AntibodyAnti-p38 MAPK antibody (Rabbit polyclonal)Cell Signaling TechnologyCat# 9212, RRID:AB_330713WB (1:2000)
AntibodyPhospho-p38 MAPK (Thr180/ Tyr182) antibody (Rabbit polyclonal)Cell Signaling TechnologyCat# 9211, RRID:AB_331641WB (1:1000)
Antibodyβ-Actin Antibody (Mouse monoclonal)Santa Cruz BiotechnologyCat# sc-47778, RRID:AB_626632WB (1:1000)
Antibodyα-Tubulin antibody (A-6) (Mouse monoclonal)Santa Cruz BiotechnologyCat# sc-398103, RRID:AB_2832217WB (1:1000)
AntibodyPurified NA/LE Hamster Anti-Mouse CD3e (Armenian Hamster monoclonal)BD BiosciencesCat# 553057, RRID:AB_394590(10 µg/mL)
AntibodyAffiniPure Goat Anti-Armenian Hamster IgG (H + L) (Goat polyclonal)Jackson ImmunoResearch LabsCat# 127-005-160, RRID:AB_2338972(25 µg/mL)
Recombinant DNA reagentpMIGR1-GFP (plasmid)Pear et al., 1998RRID:Addgene_27490
Recombinant DNA reagentpMIGR1-SPRY4-GFP (plasmid)This paperN/ACDS of mouse Spry4 was inserted into pMIGR1-GFP.
Recombinant DNA reagentpMIGR1-DUSP6-GFP (plasmid)This paperN/ACDS of mouse Dusp6 was inserted into pMIGR1-GFP.
Recombinant DNA reagentpCL-Eco (plasmid)Naviaux et al., 1996RRID:Addgene_12371Retrovirus packaging vector
Sequence-based reagentprimers used in qRT-PCR This paper See Supplementary file 3 for sequence information.
Peptide, recombinant proteinStreptavidinSouthern BiotechCat# 7100–01
Peptide, recombinant proteinStreptavidineBioscienceBD BiosciencesCat# 45-4317-80, RRID:AB_10260035Cat# 554067, RRID:AB_10050396FC (1:100)
Commercial assay or kitFixable Viability Dye eFluor 780eBioscienceCat# 65-0865-14
Commercial assay or kitGhost Dye Violet 510Tonbo BiosciencesCat# 13–0870
Commercial assay or kitFoxp3/ Transcription Factor Staining Buffer SeteBioscienceCat# 00-5523-00
Commercial assay or kitBD Cytofix Fixation BufferBD BiosciencesCat# 554655, RRID:AB_2869005
Commercial assay or kitFuGENE HD Transfection ReagentPromegaCat# E2311
Commercial assay or kitRiboExGeneAllCat# 301–002
Commercial assay or kitGoScript Reverse Transcriptase KitPromegaCat# A5001
Commercial assay or kitSYBR Green Realtime PCR Master MixTOYOBOCat# TOQPK-201
Commercial assay or kitEasySep Mouse Streptavidin RapidSpheres Isolation KitStem Cell TechnologiesCat# 19,860
Commercial assay or kitBCA Protein Assay KitPierceCat# 23,225
Commercial assay or kitClarity Western ECL SubstrateBio-RadCat# 1705061
Commercial assay or kitSuperSignal West Dura Extended Duration SubstrateThermo ScientificCat# 34,076
Chemical compound, drugIndo-1, AM, cell permeantInvitrogenCat# I1203
Chemical compound, drugIonomycin from Streptomyces conglobatusSigma-AldrichCat# I9657-1MG
Chemical compound, drugHexadimethrine bromideSigma-AldrichCat# H9268-10G
Software, algorithmFlowJoTree Star Inc.RRID:SCR_008520, https://www.flowjo.com/solutions/flowjo
Software, algorithmImageJNIHRRID:SCR_003070, https://imagej.nih.gov/ij/
Software, algorithmGraphPad Prism 7GraphPad SoftwareRRID:SCR_002798, https://www.graphpad.com/scientific- software/prism/

Additional files

Supplementary file 1

List of Differentially expressed genes (DEGs) in Cic-deficient DP thymocytes.

https://cdn.elifesciences.org/articles/71769/elife-71769-supp1-v2.docx
Supplementary file 2

Gene Ontology (GO) analysis of DEGs in Cic-deficient DP thymocytes.

https://cdn.elifesciences.org/articles/71769/elife-71769-supp2-v2.docx
Supplementary file 3

Oligonucleotide sequences used for qRT-PCR.

https://cdn.elifesciences.org/articles/71769/elife-71769-supp3-v2.docx
Supplementary file 4

Flow cytometry gating strategy.

https://cdn.elifesciences.org/articles/71769/elife-71769-supp4-v2.pdf
Transparent reporting form
https://cdn.elifesciences.org/articles/71769/elife-71769-transrepform1-v2.docx

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