A Tad-like apparatus is required for contact-dependent prey killing in predatory social bacteria
Abstract
Myxococcus xanthus, a soil bacterium, predates collectively using motility to invade prey colonies. Prey lysis is mostly thought to rely on secreted factors, cocktails of antibiotics and enzymes, and direct contac with Myxococcus cells. In this study, we show that on surfaces the coupling of A-motility and contact-dependent killing is the central predatory mechanism driving effective prey colony invasion and consumption. At the molecular level, contact-dependent killing involves a newly discovered type IV filament-like machinery (Kil) that both promotes motility arrest and prey cell plasmolysis. In this process, Kil proteins assemble at the predator-prey contact site, suggesting that they allow tight contact with prey cells for their intoxication. Kil-like systems form a new class of Tad-like machineries in predatory bacteria, suggesting a conserved function in predator-prey interactions. This study further reveals a novel cell-cell interaction function for bacterial pili-like assemblages.
Data availability
Source Data files have been provided for :- Figure 2-source data 1: E. coli loss of fluorescence during contact-dependent lysis (Figure 2c).- Figure 2-figure supplement 5-source data 1: Contact dependent-lysis and VipA-GFP dynamics.- Figure 2-figure supplement 7-source data 1: CPRG assay.- Figure 3-source data 1: CPRG assay (Figure 3b).- Figure 3-source data 2: counting percentage of contacts with a prey leading to motility pauses and prey cell lysis (Figure 3c, 3d).- Figure 3-figure supplement 3-source data 1: CPRG assay.- Figure 4-source data 1: counting percentage of contacts with a prey leading to NG-KilD foci formation and counting percentage of NG-KilD foci associated with motility pause and prey cell lysis (Figure 4e, 4f, 4g).- Figure 4-figure supplement 1-source data 1: CPRG assay.- Figure 4-figure supplement 2-source data 1: CPRG assay.- Figure 4-figure supplement 3-source data 1: Lysis time.- Figure 4-figure supplement 4-source data 1: Western Blot.- Figure 5-source data 1: Flow cytometry (Figure 5c, 5d).- Figure 5-source data 2: M. xanthus growth during prey colony invasion (Figure 5e).- Figure 5-source data 3: Increase in M. xanthus cell length during predation (Figure 5f).- Figure 5-figure supplement 2-source data 1: Growth curves.- Figure 6-source data 1: Prey CFU counts during predation (Figure 6b,c,d,e,f).- Figure 7-source data 1: Supermatrix alignment.- Figure 3-figure supplement 2: RNA-seq Data from Livingstone PG et al. (2018) Microb Genom. PMID:29345219, Supplementary File 1 available online: https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000152#supplementary_data).
Article and author information
Author details
Funding
Centre National de la Recherche Scientifique (2019 CNRS 80-Prime)
- Tâm Mignot
Ministère de l'Éducation et de l'Enseignement supérieur (MENRT thesis grant)
- Sofiene Seef
Ministère de l'Éducation et de l'Enseignement supérieur (MENRT thesis grant)
- Paul de Boissier
Ministère de l'Éducation et de l'Enseignement supérieur (MENRT thesis grant)
- Donovan Robert
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Michael T Laub, Massachusetts Institute of Technology, United States
Version history
- Preprint posted: February 27, 2021 (view preprint)
- Received: July 22, 2021
- Accepted: September 9, 2021
- Accepted Manuscript published: September 10, 2021 (version 1)
- Version of Record published: September 23, 2021 (version 2)
Copyright
© 2021, Seef et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Further reading
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Background:
Few national-level studies have evaluated the impact of ‘hybrid’ immunity (vaccination coupled with recovery from infection) from the Omicron variants of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
Methods:
From May 2020 to December 2022, we conducted serial assessments (each of ~4000–9000 adults) examining SARS-CoV-2 antibodies within a mostly representative Canadian cohort drawn from a national online polling platform. Adults, most of whom were vaccinated, reported viral test-confirmed infections and mailed self-collected dried blood spots (DBSs) to a central lab. Samples underwent highly sensitive and specific antibody assays to spike and nucleocapsid protein antigens, the latter triggered only by infection. We estimated cumulative SARS-CoV-2 incidence prior to the Omicron period and during the BA.1/1.1 and BA.2/5 waves. We assessed changes in antibody levels and in age-specific active immunity levels.
Results:
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Conclusions:
Strategies to maintain population-level hybrid immunity require up-to-date vaccination coverage, including among those recovering from infection. Population-based, self-collected DBSs are a practicable biological surveillance platform.
Funding:
Funding was provided by the COVID-19 Immunity Task Force, Canadian Institutes of Health Research, Pfizer Global Medical Grants, and St. Michael’s Hospital Foundation. PJ and ACG are funded by the Canada Research Chairs Program.
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