Cannabidiol activates neuronal Kv7 channels
- Department of Neurobiology, Harvard Medical School, United States
- F.M. Kirby Neurobiology Research Center, Boston Children's Hospital, United States
- ICCB-Longwood Screening Facility and Department of Immunology, Harvard Medical School, United States
Figures
Figure 1
Cannabidiol (CBD) enhancement of cloned human Kv7.2/7.3 channel current in Chinese hamster ovary (CHO) cells.
(A) hKv7.2/7.3 current evoked by staircase depolarizations before and after application of 100 nM CBD. (B) Collected results (mean ± SEM) for current at –50 mV after application of 100 nM (n = 20) …
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Figure 1—source data 1
Screen data and source data for Figure 1.
- https://cdn.elifesciences.org/articles/73246/elife-73246-fig1-data1-v1.xlsx
Figure 2
Cannabidiol (CBD) enhancement of M-current in mouse sympathetic neurons.
(A) Currents evoked by hyperpolarizations to –60 mV, –70 mV, and –80 mV from a holding potential of –30 mV before (blue) and after (red) application of 100 nM CBD. (B) Collected results (mean ± SEM) …
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Figure 2—source data 1
Source data for Figure 2.
- https://cdn.elifesciences.org/articles/73246/elife-73246-fig2-data1-v1.xlsx
Figure 3
Cannabidiol (CBD) enhancement of Kv7 current in rat hippocampal neurons.
(A) Currents at a holding voltage of –30 mV and during a 500 ms hyperpolarization to –50 mV in control, after application of 1 μM CBD, and after addition of 3 μM XE-991 in the continuing presence of …
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Figure 3—source data 1
Source data for Figure 3.
- https://cdn.elifesciences.org/articles/73246/elife-73246-fig3-data1-v1.xlsx
Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | Swiss Webster | Charles River | Cat# 024 | |
| Strain, strain background (Rattus norvegicus) | Sprague–Dawley | Charles River | Cat# 400 | |
| Cell line (Cricetulus griseus) | Kv7.2/7.3 CHO cell line | Mayflower Bioscience | BSYS-KV7.2/3-CHO-C | CHO (Chinese hamster [C. griseus ] ovary) cell line stably transfected with recombinant human Kv7.2/7.3 ion channels |
| Commercial assay or kit | MycoAlert PLUS Mycoplasma Detection Kit | Lonza | LT07-703 | |
| Commercial assay or kit | FluxOR II Green Potassium Ion Channel Assay | Invitrogen | LT07-703 | |
| Chemical compound, drug | Cannabidiol | Cayman Chemical | Cat# 90080, CAS 13956-29-1 | |
| Chemical compound, drug | Ham’s F12-Glutamax-l medium | Gibco | Cat# 31765-035 | |
| Chemical compound, drug | Penicillin-streptomycin | Gibco | Cat# 15140-122 | |
| Chemical compound, drug | Puromycin | InvivoGen | Cat# ant-pr-1 | |
| Chemical compound, drug | Papain | Worthington Biochemical | Cat# LS003126 | |
| Chemical compound, drug | L-15 | Gibco | Cat# 11415-064 | |
| Chemical compound, drug | Neurobasal A Medium | Gibco | Cat# 10888-022 | |
| Chemical compound, drug | B-27 | Gibco | Cat# 17504-010 | |
| Chemical compound, drug | Penicillin-streptomycin | Sigma-Aldrich | Cat# P4333 | |
| Chemical compound, drug | Minimal Essential Medium | American Tissue Type Collection | Cat# DMEM 30-2002 | |
| Chemical compound, drug | Hank’s Balanced Salt Solution | Gibco | Cat# 14170-112 | |
| Chemical compound, drug | DMEM/F12 | Gibco | Cat# 11330-032 | |
| Chemical compound, drug | Tetrodotoxin w/citrate | Abcam | Ab120055 | |
| Software, algorithm | Clampex | Molecular Devices | Version 10.3.1.5 | https://www.moleculardevices.com |
| Software, algorithm | Igor Pro | WaveMetrics | Version 6.12A | https://www.wavemetrics.com |
| Software, algorithm | DataAccess | Bruxton Corporation | http://www.bruxton.com/DataAccess/index.html |
