Golden Syrian hamster as a model to study cardiovascular complications associated with SARS-CoV-2 infection
- Immuno-biology Lab, Infection and Immunology Centre, Translational Health Science and Technology Institute, NCR-Biotech Science Cluster, India
- Immunology Core, Translational Health Science and Technology Institute, NCR-Biotech Science Cluster, India
- Non-communicable Disease Centre, Translational Health Science and Technology Institute, NCR-Biotech Science Cluster, India
- Infection and Immunology Centre, Translational Health Science and Technology Institute, NCR-Biotech Science Cluster, India
Figures
Figure 1 with 1 supplement
Pulmonary pathologies of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)-infected hamsters.
Hamsters infected with 104 or 105 plaque-forming unit (PFU) SARS-CoV-2 and the course of infection were followed for 14 days with the evaluation of clinical and pathological features of SARS-CoV-2 …
Figure 1—figure supplement 1
Computational analysis deciphering the structural and interaction level insights of key host proteins, angiotensin-converting enzyme 2 (ACE2) (A–E) and neuropilin-1 (NRP-1) (F, G) involved in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection.
(A) The sequence alignment of human (hu) and hamster (ha) ACE2 sequences. Along with secondary structure, the residual variations are highlighted in yellow arrows indicating the differences among …
Figure 2 with 1 supplement
Immunological response against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection in hamsters.
Immunological responses of SARS-CoV-2-challenged hamsters were evaluated from serum or spleen samples. (A) Changes in spleen length of SARS-CoV-2-infected animals at different days post infection …
Figure 2—figure supplement 1
Immunopathological changes associated with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection in hamsters.
(A) IgG titer for day 14 post-infection serum samples for different doses of infection. (B) FACS dot plot and bar graph showing mean ± SEM % frequency of CD4+ cells (one-way ANOVA, nonparametric …
Figure 3 with 1 supplement
Cardiovascular complication (CVC) in hamsters challenged with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Histopathological changes in infected hamsters along with serum markers for CVC were evaluated. (A, B) Images of hematoxylin and eosin (HE)-stained heart captured at 1× and 10× showing ventricular …
Figure 3—figure supplement 1
Pathological changes in hearts of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)-infected hamsters.
(A) Heart ventricular hypertrophy on 7 days post infection (dpi). (B) Ventricular mass by body mass ratio. (C) Masson’s trichrome (MT)-stained heart image from the experimental control sample. (D) …
Figure 4
Serum lipid profile of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)-infected hamsters.
Lipid profiles from the serum samples of infected and uninfected hamsters were evaluated by biochemical and liquid chromatography-tandem mass spectrometry (LC/MS-MS) profiling. (A) Serum lipid …
Figure 5
Serum metabolomics profile of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)-infected hamsters.
(A) Schematic representation of metabolomics analysis from the serum samples of infected and uninfected hamsters. (B) Venn diagrams for differentially regulated metabolites identified in this study …
Figure 6
Identification of serum metabolic biomarker for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)-infected hamsters.
Serum metabolomics profile obtained from hamster study was then used to overlay with previously published and in-house-generated metabolomics profile database to identify serum metabolomics …
Figure 7
