Drosophila nicotinic acetylcholine receptor subunits and their native interactions with insecticidal peptide toxins
- Department of Genetics, University of Cambridge, Downing Street, United Kingdom
- Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, United Kingdom
- Syngenta, Jealott's Hill International Research Centre, United Kingdom
- MRC Toxicology Unit, Gleeson Building, University of Cambridge, Tennis Court Road, United Kingdom
- Cambridge Advanced Imaging Centre, Department of Physiology, Development and Neuroscience/Anatomy Building, University of Cambridge, United Kingdom
- Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Tennis Court Road, United Kingdom
Figures
Figure 1 with 1 supplement
Morphological and locomotor phenotypes in nAChR subunit mutants.
(A) Adult males from indicated nAChR subunit null mutants, black arrows indicate curled abdomens but even in lines with a low frequency the phenotype is prominent (blue arrow). (B) Frequency of …
Figure 1—figure supplement 1
Construction of new D. melanogaster nicotinic acetylcholine receptor subunit gene mutations.
(A) Diagram of the construct used to generate DsRED insertions into each of the nAChR subunit genes. LHA and RHA = Left and Right homology arms. (B) A guide RNA target site is identified in the exon …
Figure 2
ω-Hexatoxin-Hv1a and α-Bungarotoxin target different nAChR subunits.
(A) Bar graph of the survival rate, measured as the percentage of pupae formed, following larval injection of 2.5 nmol/g Hv1a in the indicated homozygous lines. **p=0.0035 (one-way ANOVA F(11,24) = …
Figure 3 with 1 supplement
Forming styrene maleic acid lipid particles (SMALPs).
(A) Schematic representation of the SMALPs extraction and nAChRs pull-down for mass spectrometric analysis. (B) Negative staining of extracted SMALPs by transmission electron microscopy, n=3. Scale …
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Figure 3—source data 1
Transmission electron microscopy micrographs.
- https://cdn.elifesciences.org/articles/74322/elife-74322-fig3-data1-v1.zip
Figure 3—figure supplement 1
Coupling α-Btx and testing pull-down efficiency with affinity beads.
(A) Fluorescence signal of uncoupled α-Btx in solution before and after coupling to affinity beads (two-tailed t-test, ***p<0.001, n=4). (B) Biological replicate: same condition as in Figure 3C, …
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Figure 3—figure supplement 1—source data 1
Coupling efficiency of α-Btx to affinity beads.
- https://cdn.elifesciences.org/articles/74322/elife-74322-fig3-figsupp1-data1-v1.xlsx
Figure 4 with 1 supplement
Identification of proteins enriched by SMALP extraction.
(A) Number of identified proteins in affinity pull-down samples solubilised with or without SMA, two-tailed t-test, **p<0.01, n=6 or 8 replicates per condition. (B) MS/MS spectrum counts from …
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Figure 4—source data 1
SMALP extraction raw data.
- https://cdn.elifesciences.org/articles/74322/elife-74322-fig4-data1-v1.xlsx
Figure 4—figure supplement 1
GO terms and predicted membrane proteins.
(A) GO slim term for biological process and (B) for molecular function analysed within samples solubilised without or with SMA, n=4 or 11 per conditions. Predicted β-barrel membrane- (C), two-tailed …
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Figure 4—figure supplement 1—source data 1
Further SMALP extraction raw data.
- https://cdn.elifesciences.org/articles/74322/elife-74322-fig4-figsupp1-data1-v1.xlsx
Figure 5 with 2 supplements
Three nAChR α-subunits bind to α-Bungarotoxin (α-Btx).
(A) Identified Dα5, Dα6, and Dα7 subunit peptides in pull-downs using α-Btx affinity beads. Peptides from the ligand-binding and cytoplasmic domain are highlighted in red. (B) Numbers of identified …
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Figure 5—source data 1
Null pull-down raw data.
- https://cdn.elifesciences.org/articles/74322/elife-74322-fig5-data1-v1.xlsx
Figure 5—figure supplement 1
Illustration of nAChR subunits.
(A) Graphical representation of ten nAChR subunits. The position of protein domains and signal peptides are shown.
Figure 5—figure supplement 2
Identified peptides in ligand-binding and cytoplasmic domain.
(A) Shared peptides found in the ligand-binding domains are shown in red. (B) Identified unique (/) and shared (,) peptides in cytoplasmic domain.
Figure 6 with 2 supplements
N-glycosylation sites in nAChR subunits.
(A) Diagrammatic representation of nAChR subunit glycopeptide enrichment. Pull-downs with α-Btx affinity beads enrich for nAChRs and after tryptic digestion glycopeptides were enriched. …
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Figure 6—source data 1
Glycopeptide raw data.
- https://cdn.elifesciences.org/articles/74322/elife-74322-fig6-data1-v1.xlsx
Figure 6—figure supplement 1
MS/MS spectra of Da5 and Da7 subunit peptides.
(A) MS/MS spectra of fragmented shared Dα5 and Dα7 subunit peptides. Glycopeptides were deglycosylated with Endo H and the second asparagine (N) residue is modified with an N-acetylhexosamine, …
Figure 6—figure supplement 2
Glycosylation sites of nAChR subunits.
(A) Multiple sequence alignment of insect α7 nAChR subunits compared to sequences of nematodes. The glycosylated ligand-binding domain (LBD) sequence of Dα5 and Dα7 nAChR subunits are shown. …
Figure 7 with 1 supplement
In vivo imaging of endogenously tagged Dα6 nAChR subunit.
(A–C) Dα6-YFP localisation from live confocal imaging in 2nd A, early B and late C 3rd instar larval brains. Visible localisation in brain (Br), ventral nerve cord (VNC), mushroom bodies (MB), and …
Figure 7—figure supplement 1
In vivo imaging of endogenously tagged Dα1, Dβ1, Dβ2 nAChR subunits.
Live confocal microscope images for the indicated subunits tagged at the C-terminus with YFP. Scale bars as indicated.
Videos
Video 1
Late 3rd instar larvae, with defined expression of Dα6-YFP in the Kenyon cells, calyx, peduncle, dorsal and medial lobes.
Scale bar = 100 µm.
Video 2
Third instar larvae, with detectable fluorescence signal in Kenyon cells, calyx, peduncle, dorsal lobes and medial lobes.
Scale bar = 100 µm.
Video 3
Later 3rd instar larvae, with signal of Dα6-YFP in developing optic lobes, lamina (Lam) and medulla (Med).
Scale bar = 100 µm.
Video 4
Adult brain, expression of Da6-YFP is largely restricted to the mushroom bodies.
Scale bar = 100 µm.
Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent | pCDF3 | Port et al., 2014 | N/A | |
| Genetic reagent | FSVS | Korona et al., 2020 | N/A | |
| Genetic reagent | 3 × P3-DsRED | Gratz et al., 2014 | N/A | |
| Genetic reagent (D. melanogaster) | {nos-Cas9}attP40 | Ren et al., 2013 | BDSC:78,781 | |
| Genetic reagent (D. melanogaster) | {nos-Cas9}attP2 | Ren et al., 2013 | BDSC:78,782 | |
| Genetic reagent (D. melanogaster) | w1118 | FlyBase | FBal0018186 | |
| Genetic reagent (D. melanogaster) | nAChRα1 KO | This study | N/A | Steven Russell/ https://www.flyfacility.gen.cam.ac.uk |
| Genetic reagent (D. melanogaster) | nAChRα2 KO | This study | N/A | Steven Russell/ https://www.flyfacility.gen.cam.ac.uk |
| Genetic reagent (D. melanogaster) | nAChRα3 KO | This study | N/A | Steven Russell/ https://www.flyfacility.gen.cam.ac.uk |
| Genetic reagent (D. melanogaster) | nAChRα4 KO | This study | N/A | Steven Russell/ https://www.flyfacility.gen.cam.ac.uk |
| Genetic reagent (D. melanogaster) | nAChRα5 KO | This study | N/A | Steven Russell/ https://www.flyfacility.gen.cam.ac.uk |
| Genetic reagent (D. melanogaster) | nAChRα6 KO | This study | N/A | Steven Russell/ https://www.flyfacility.gen.cam.ac.uk |
| Genetic reagent (D. melanogaster) | nAChRα7 KO | This study | N/A | Steven Russell/ https://www.flyfacility.gen.cam.ac.uk |
| Genetic reagent (D. melanogaster) | nAChRβ2 KO | This study | N/A | Steven Russell/ https://www.flyfacility.gen.cam.ac.uk |
| Genetic reagent (D. melanogaster) | nAChRβ3 KO | This study | N/A | Steven Russell/ https://www.flyfacility.gen.cam.ac.uk |
| Genetic reagent (D. melanogaster) | nAChRα1FSVS | This study | N/A | Steven Russell/ https://www.flyfacility.gen.cam.ac.uk |
| Genetic reagent (D. melanogaster) | nAChRα6FSVS | This study | N/A | Steven Russell/ https://www.flyfacility.gen.cam.ac.uk |
| Genetic reagent (D. melanogaster) | nAChRβ1FSVS | This study | N/A | Steven Russell/ https://www.flyfacility.gen.cam.ac.uk |
| Genetic reagent (D. melanogaster) | nAChRβ2FSVS | This study | N/A | Steven Russell/ https://www.flyfacility.gen.cam.ac.uk |
| Genetic reagent (E. coli) | E. coli competent cells | New England Biolabs | Catalogue number: C2987H | |
| Commercial assay or kit | BbsI | New England Biolabs | Catalogue number: R0539 | |
| Commercial assay or kit | Gibson Assembly Master Mix | New England Biolabs | Catalogue number: E2611L | |
| Commercial assay or kit | Q5 High-Fidelity 2 X Master Mix | New England Biolabs | Catalogue number: M0492L | |
| Commercial assay or kit | Pierce fluorometric peptide kit | Thermo Scientific | Catalogue number: 23,290 | |
| Peptide, recombinant protein | Trypsin/Lys-C mix | Promega | Catalogue number: V5073 | |
| Antibody | Anti-GFP (goat monoclonal) | Abcam | Catalogue number: Ab252881 | 1:1,000 |
| Antibody | Anti-rat IgG (goat polyclonal) | Sigma-Aldrich | Catalogue number: A9037 | 1:10.000 |
| Peptide, recombinant protein | ω‐hexatoxin‐Hv1a | Syngenta | CH-4332 Stein, Switzerland | |
| Peptide, recombinant protein | α-Bungarotoxin | Abcam | Catalogue number: ab120542 | |
| Peptide, recombinant protein | Carbachol | Insight Biotechnology Ltd | Catalogue number: CAS 51-83-2 | |
| Other | CNBr-activated sepharose | Sigma-Aldrich | Catalogue number: C9 142–5 G | |
| Other | Styrene maleic acid copolymer (3:1) | Dafforn, p.c. | ||
| Commercial assay or kit | Mini-Protean TGX precast gels | Bio-Rad Laboratories | Catalogue number: 456–1,084 | |
| Commercial assay or kit | ECL-Chemiluminescent detection solution | GE Healthcare | Catalogue number: 45-000-999 | |
| Commercial assay or kit | CL-XPosure films | Thermo Scientific | Catalogue number: 10465145 | |
| Software, algorithm | GraphPad Prism | GraphPad Software | RRID:SCR_002798 | |
| Software, algorithm | Proteome Discoverer 2.3 | Thermo Scientific | RRID:SCR_014477 |
Additional files
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Supplementary file 1
Abdomen phenotype.
- https://cdn.elifesciences.org/articles/74322/elife-74322-supp1-v1.docx
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Supplementary file 2
Climbing ability.
- https://cdn.elifesciences.org/articles/74322/elife-74322-supp2-v1.docx
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Supplementary file 3
Drosophila larval injection of ω-Hexatoxin-Hv1a & α-Bungarotoxin.
- https://cdn.elifesciences.org/articles/74322/elife-74322-supp3-v1.docx
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Supplementary file 4
Identified nAChR peptides in pull-downs with α-Bungarotoxin.
Peptides from Dα3, Dα5, Dα6, Dα7 and Dβ3 nAChR subunits are listed and found [N] times within individual replicates. Protein domains are marked with: Ed extracellular-, Id Intracellular-, LBD ligand-binding-, and Non-domain localization. The mass-to-charge ratio (m/z) of the precursor ions, the protonated monoisotopic masses, the theoretical MH+ masses in Dalton [Da] and peptide modifications are listed. Peptide modifications are listed with: (C) Carbamidomethylation; (N,Q) Deamidation; (H) N-acetylhexosamine (HexNAc); (M) Oxidation.
- https://cdn.elifesciences.org/articles/74322/elife-74322-supp4-v1.docx
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Supplementary file 5
Identified nAChR peptides in pull-downs without α-Bungarotoxin.
Identified peptides of nAChR subunits which are found in control pull-down samples without α-Bungarotoxin (α-Btx).
- https://cdn.elifesciences.org/articles/74322/elife-74322-supp5-v1.docx
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Supplementary file 6
List of gRNAs and oligonucleotides used for cloning.
- https://cdn.elifesciences.org/articles/74322/elife-74322-supp6-v1.docx
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Supplementary file 7
List of oligonucleotides used for amplification from genomic DNA.
- https://cdn.elifesciences.org/articles/74322/elife-74322-supp7-v1.docx
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Supplementary file 8
C-terminal tagging of nAChRa6 with FSVS.
- https://cdn.elifesciences.org/articles/74322/elife-74322-supp8-v1.docx
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Supplementary file 9
Wet pellet weight of membrane fractions.
- https://cdn.elifesciences.org/articles/74322/elife-74322-supp9-v1.docx
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Transparent reporting form
- https://cdn.elifesciences.org/articles/74322/elife-74322-transrepform1-v1.pdf
