Distinct dendritic Ca2+ spike forms produce opposing input-output transformations in rat CA3 pyramidal cells
- Laboratory of Neuronal Signaling, Institute of Experimental Medicine, Hungary
- János Szentágothai School of Neurosciences, Semmelweis University, Hungary
Figures
Figure 1 with 1 supplement
Dendritic Na+ spikes in dual recordings of soma and dendrite of CA3 pyramidal cells (CA3PCs).
(a) Two-photon (2P) collapsed z-stack image of a dually recorded CA3PC (dendritic pipette distance: 255 μm). Somatic pipette outlined in blue. Inset: soma (red arrowhead) located in CA3. (b) Somatic …
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Figure 1—source data 1
Dendritic Na+ spikes in dual recordings of soma and dendrite of CA3 pyramidal cells (CA3PCs).
- https://cdn.elifesciences.org/articles/74493/elife-74493-fig1-data1-v1.xlsx
Figure 1—figure supplement 1
Characteristics of different regenerative dendritic events.
(a) Representative trace from a dual recording, showing the lack of distal dendritic Ca2+ signal associated with a putative dendritic Na+ spike (dark blue arrow). (b) Voltage and corresponding dV/dt …
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Figure 1—figure supplement 1—source data 1
Characteristics of different regenerative dendritic events.
- https://cdn.elifesciences.org/articles/74493/elife-74493-fig1-figsupp1-data1-v1.xlsx
Figure 2
Diverse dendritic Ca2+ spike forms in soma-dendrite dual recordings from CA3 pyramidal cells (CA3PCs).
(a) Schematic of experiments. Green line indicates typical Ca2+ imaging site distal from the dendritic patch. (b) Representative recording of a slow afterdepolarization (ADP). Dendritic (black) and …
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Figure 2—source data 1
Diverse dendritic Ca2+ spike forms in soma-dendrite dual recordings from CA3 pyramidal cells (CA3PCs).
- https://cdn.elifesciences.org/articles/74493/elife-74493-fig2-data1-v1.xlsx
Figure 3 with 3 supplements
Characterization of dendritic Ca2+ spike types.
(a) Schematic of dendrite-only experiments. (b) Representative dendritic voltage and Ca2+ recording of afterdepolarization (ADP) (left) and dendritically initiated (DI) spike (right) from two …
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Figure 3—source data 1
Characterization of dendritic Ca2+ spike types.
- https://cdn.elifesciences.org/articles/74493/elife-74493-fig3-data1-v1.xlsx
Figure 3—figure supplement 1
Variability of Ca2+ spike phenotypes across and within CA3 pyramidal cells (CA3PCs).
(a–c) Additional examples of CA3PCs expressing variable forms of dendritic Ca2+ spikes. (a) A dendrite expressing both (i) afterdepolarizations (ADPs) with slow and fast kinetics, as well as (ii) …
Figure 3—figure supplement 2
Dendritic Ca2+ spike properties do not depend on excitatory synaptic activity.
(a) Representative voltage responses to Iinj in a CA3PC dendrite with afterdepolarization (ADP) (top) and another CA3PC dendrite with dendritically initiated (DI) spike (bottom) under control …
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Figure 3—figure supplement 2—source data 1
Dendritic Ca2+ spike properties do not depend on excitatory synaptic activity.
- https://cdn.elifesciences.org/articles/74493/elife-74493-fig3-figsupp2-data1-v1.xlsx
Figure 3—figure supplement 3
Dendritic Ca2+ spikes in CA1 pyramidal cells (CA1PCs) are afterdepolarization (ADP) type.
(a) Two-photon (2P) stack of a CA1PC loaded with OGB-1 and Alexa 594 via a dendritic patch pipette. (b) Dendritic Vm (upper) and distal dendritic Ca2+ (lower) traces measured in the cell shown in (a)…
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Figure 3—figure supplement 3—source data 1
Dendritic Ca2+ spikes in CA1 pyramidal cell (CA1PCs) are afterdepolarization (ADP) type.
- https://cdn.elifesciences.org/articles/74493/elife-74493-fig3-figsupp3-data1-v1.xlsx
Figure 4
Properties of dendritically initiated (DI) Ca2+ spikes.
(a) Spike amplitude as a function of pipette distance from soma (circles: individual dendrites, n = 30). (b) Width at half amplitude of DI spikes. Open circles: individual dendrites; filled symbol: …
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Figure 4—source data 1
Properties of DI Ca2+ spikes.
- https://cdn.elifesciences.org/articles/74493/elife-74493-fig4-data1-v1.xlsx
Figure 5 with 1 supplement
Ca2+ spikes with different kinetics are mediated by different ionic mechanisms.
(a, b) Representative experiments showing the effect of 1 μM TTX on afterdepolarizations (ADPs) (a) and dendritically initiated (DI) spikes (b). Vm (top) and distal dendrite Ca2+ signals (bottom) …
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Figure 5—source data 1
Ca2+ spikes with different kinetics are mediated by different ionic mechanisms.
- https://cdn.elifesciences.org/articles/74493/elife-74493-fig5-data1-v1.xlsx
Figure 5—figure supplement 1
Additional properties of dendritic Ca2+ spikes.
(a) Example recording of a CA3PC dendrite with mixed types of Ca2+ spikes in TTX. (b) Example recording of a CA3PC dendrite with no clear Ca2+ spike. (c) Example recording of a CA3PC dendrite with …
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Figure 5—figure supplement 1—source data 1
Additional properties of dendritic Ca2+ spikes.
- https://cdn.elifesciences.org/articles/74493/elife-74493-fig5-figsupp1-data1-v1.xlsx
Figure 6
Propagation of different Ca2+ spike types.
(a) Schematic of experimental strategy. Dendritic Ca2+ signals were measured as a proxy to assess Ca2+ spike propagation in different regions of the arbor. (b) Representative TTX-resistant slow …
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Figure 6—source data 1
Propagation of different Ca2+ spike types.
- https://cdn.elifesciences.org/articles/74493/elife-74493-fig6-data1-v1.xlsx
Figure 7
Dendritic Ca2+ spike phenotype correlates with morphological traits (a, b).
Representative two-photon (2P) stack of two CA3 pyramidal cells (CA3PCs) with afterdepolarization (ADP)-only (a) or dendritically initiated (DI) (b) Ca2+ spike phenotypes (with ≤600 or 700 pA Iinj). …
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Figure 7—source data 1
Dendritic Ca2+ spike phenotype correlates with morphological traits.
- https://cdn.elifesciences.org/articles/74493/elife-74493-fig7-data1-v1.xlsx
Figure 8 with 1 supplement
Opposing impact of different Ca2+ spike types on somatic output.
(a) Example of action potential (AP) output evoked by slow afterdepolarization (ADP) (top), fast ADP (middle), and dendritically initiated (DI) spike (bottom) in dual recordings (three different …
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Figure 8—source data 1
Opposing impact of different Ca2+ spike types on somatic output.
- https://cdn.elifesciences.org/articles/74493/elife-74493-fig8-data1-v1.xlsx
Figure 8—figure supplement 1
Effect of dendritically initiated (DI) spikes on action potential (AP) output in single-site dendritic recordings.
(a) Experiment schematic. (b) Three individual trace segments during 200 pA Iinj steps recorded in a representative CA3 pyramidal cell (CA3PC) dendrite, showing s-APs and Ca-APs. (c) Ca-APs (orange, …
