(A) Schematic view of the outer tissues of an adult C. elegans worm near the mid-body, highlighting the furrows that separate the annuli, the different collagen genes in the annuli (green) or furrow (blue) and indicating the region analysed with the red dashed rectangle. (B, C) L1 larvae expressing VHA-5::GFP [Si] were treated with the indicated RNAi clones and analysed at the young adult stage: control (sta-1), annuli collagens dpy-4, dpy-5 and dpy-13 (green) and the furrow collagens: dpy-2, dpy-3, dpy-7, dpy-8, dpy-9, or dpy-10 (blue). (B) Confocal images of selected regions on the lateral epidermis, at a constant position relative to the vulva, scale bar, 5 µm. (C) Quantification of VHA-5 signal average size, Feret’s diameter and density. All statistics are in Figure 6—source data 1, ****p<0.0001 and ***p<0.001. Control: sta-1 (n = 17), dpy-4 (n = 11), dpy-5 (n = 10), dpy-13 (n = 11); furrow Dpy: dpy-2 (n = 11) dpy-3 (n = 10), dpy-7 (n = 14), dpy-8 (n = 13), dpy-9 (n = 13), or dpy-10 (n = 11).