(A) Overview of the C. elegans alimentary system from Wormbook (Hall and Altun, 2007), with neuronal cell bodies in the pharynx added in red. (B) Projection patterns of pharyngeal neurons within the …
(A) ceh-33 and ceh-34 loci showing different alleles and fosmid reporters used in this study. (B) Expression of the ceh-34 CRISPR/Cas-9-engineered reporter allele ot903 over the course of …
(A) Phylogeny of ceh-33/34 gene duplication in nematodes. Phylogeny based on homeodomains. (B) Synteny of the ceh-33/ceh-34 locus across several Caenorhabditis species.
(A) pha-4 expression (stIs10077) is not affected in ceh-34(tm3733) mutants. Animals were scored at the L1 stage. Statistical analysis was performed using unpaired t-test. (B) pha-4 is continuously …
(A) Pictures at the L1 stage showing expression of pan-neuronal reporter transgenes that monitor ric-4 (otIs350), ric-19 (otIs380), unc-11 (otIs620), and rab-3 (otIs291) expression. A single focal …
(A) Representative pictures and quantification showing neurotransmitter receptor expression loss in ceh-34 mutants. Reporter genes used are transgenes glr-2 (ivIs26), mgl-1 (otIs341), and a …
(A) Representative pictures and quantification showing expression of 10 different neuropeptides is affected in ceh-34 mutants. Reporter genes used are transgenic reporters for flp-2 (ynIs57), flp-4 …
(A) Representative pictures and quantification showing sensory receptor expression loss in ceh-34 mutants. Reporter transgenes used are glr-7 (otIs809), gur-3 (nIs780), and str-97 (otIs716). str-97 …
scRNA data was extracted from the Cengen App and displayed in a heatmap format, as previously described (Taylor et al., 2021). The F13H10.6 gene was included here as well because of its striking …
Representative pictures and quantification are shown. Reporter gene used is ceh-28 (nIs175). Twenty percent of worms show a cell outside the pharynx ectopically expressing ceh-28. Animals were …
(A) Schematic of the AIDv2 system (Hills-Muckey et al., 2022). Skp1, Cul1, Rbx1, and E2 are phylogenetically conserved components of the E3 ligase complex. TIR1F79G is a modified plant-specific …
(A) ceh-34 null mutants display disorganized axodendritic projections. Axodendritic projections were scored as a whole rather than by individual neuron because with all the pharyngeal neurons being …
Synchronized population of worms at the L1 or young adult stage were transferred onto ethanol or 5-Ph-IAA-coated plates and scored 48 hr later. Worms were expressing TIR1F79G ubiquitously under the r…
(A) Effects of slt-1 on the I2/I4 neurons. Orange arrows indicate position of pharyngeal nerve ring. Blue arrow indicates ectopic projection. The gur-3 reporter array is nIs780. (B) Effects of slt-1 …
(A) eya-1 is expressed in all pharyngeal neurons throughout the life of the worm. Images of L1 and adult worms showing co-localization of eya-1 expression (nIs352) with the pan-neuronal gene rab-3 (o…
(A) unc-86 and ceh-34 synergistically affect NSM differentiation. Representative images and quantification are shown. Reporter gene used is cat-1 (otIs224). Animals were scored at the L4 stage. …
(A) ceh-2 affects M3 neuron differentiation. Representative images and quantification are shown. Reporter gene is eat-4 (otIs388). Animals scored at the L4 stage. Statistical analysis was performed …
(A) Homeobox mutant alleles generated in this study. Numbers indicating beginning and end of deletion are relative to the start codon of the gene of interest. See Materials and methods for detail. (B…
We misexpressed genomic copies of the unc-86 and ttx-3 locus, driven by the pan-pharyngeal neuron promoter ehs-1 in several independent transgenic lines to generate the normally NSM-specific …
Strain list.
This file provides a list of all Caenorhabditis elegans strains used in this study.
This file provides all the primary animal scoring data.