(A) Definition of the distance (df, db), time (tf, tb), and velocity (vf, vb) used for the histograms in (B – D). (B – D) Histograms of the distance (B), time (C), and velocity (D) during the forward (df, tf, vf) and backward (db, tb, vb) movements of the bead attached to the dynein-MT-DNA-origami complex. Average (mean ± SEM) and median values are indicated in each histogram (n=35 for df, tf, vf, and 21 for db, tb, db). (E) A histogram of the frequency of the oscillatory movements (n=29). (F) Histograms of the step size during unidirectional movement (top; n=211) and oscillatory movement (bottom; n=72 for forward and n=44 for backward), fit with multiple Gaussian curves. Main peak positions of the Gaussian functions are indicated. Also see Figure 6—source data 1.