1. Biochemistry and Chemical Biology

A native chemical chaperone in the human eye lens

  1. Eugene Serebryany
  2. Sourav Chowdhury
  3. Christopher N Woods
  4. David C Thorn
  5. Nicki E Watson
  6. Arthur A McClelland
  7. Rachel E Klevit
  8. Eugene I Shakhnovich  Is a corresponding author
  1. Harvard University, United States
  2. University of Washington, United States
https://doi.org/10.7554/eLife.76923
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Cite this article
  1. Eugene Serebryany
  2. Sourav Chowdhury
  3. Christopher N Woods
  4. David C Thorn
  5. Nicki E Watson
  6. Arthur A McClelland
  7. Rachel E Klevit
  8. Eugene I Shakhnovich
(2022)
A native chemical chaperone in the human eye lens
eLife 11:e76923.
https://doi.org/10.7554/eLife.76923
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Abstract

Cataract is one of the most prevalent protein aggregation disorders and still the most common cause of vision loss worldwide. The metabolically quiescent core region of the human lens lacks cellular or protein turnover; it has therefore evolved remarkable mechanisms to resist light-scattering protein aggregation for a lifetime. We now report that one such mechanism involves an unusually abundant lens metabolite, myo-inositol, suppressing aggregation of lens crystallins. We quantified aggregation suppression using our previously well-characterized in vitro aggregation assays of oxidation-mimicking human γD-crystallin variants and investigated myo-inositol's molecular mechanism of action using solution NMR, negative-stain TEM, differential scanning fluorometry, thermal scanning Raman spectroscopy, turbidimetry in redox buffers, and free thiol quantitation. Unlike many known chemical chaperones, myo-inositol's primary target was not the native, unfolded, or final aggregated states of the protein; rather, we propose that it was the rate-limiting bimolecular step on the aggregation pathway. Given recent metabolomic evidence that it is severely depleted in human cataractous lenses compared to age-matched controls, we suggest that maintaining or restoring healthy levels of myo-inositol in the lens may be a simple, safe, and globally accessible strategy to prevent or delay lens opacification due to age-onset cataract.

Data availability

Numerical data and fits used to generate the plots and fits of Figure 1B,C,D,E have been uploaded as source data files. Raw numerical measurements behind the graphs presented in Figure 4 are included as a supplementary table (Figure 4-source data 1). All raw TEM images and instrument files have been uploaded to the Dataverse repository and are available at: https://doi.org/10.7910/DVN/BVRS9M. Full unedited gels used to generate Figure 6 - figure supplement 1 were uploaded as source data. The numerical data and fits used to generate Figure 6D were uploaded as a source data file. All other data are contained in the manuscript.

Article and author information

Author details

  1. Eugene Serebryany

    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-1066-7143

  2. Sourav Chowdhury

    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, United States
    Competing interests
    The authors declare that no competing interests exist.

  3. Christopher N Woods

    Department of Biochemistry, University of Washington, Seattle, United States
    Competing interests
    The authors declare that no competing interests exist.

  4. David C Thorn

    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-7332-2292

  5. Nicki E Watson

    Center for Nanoscale Systems, Harvard University, Cambridge, United States
    Competing interests
    The authors declare that no competing interests exist.

  6. Arthur A McClelland

    Center for Nanoscale Systems, Harvard University, Cambridge, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-4798-5954

  7. Rachel E Klevit

    Department of Biochemistry, University of Washington, Seattle, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-3476-969X

  8. Eugene I Shakhnovich

    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, United States
    For correspondence
    shakhnovich@chemistry.harvard.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-4769-2265

Funding

National Institutes of Health (R01EY030444)

  • Sourav Chowdhury
  • David C Thorn
  • Eugene I Shakhnovich

National Institutes of Health (F32GM126651)

  • Eugene Serebryany

National Institutes of Health (K99GM141459)

  • Eugene Serebryany

National Institutes of Health (R01EY017370)

  • Christopher N Woods
  • Rachel E Klevit

National Science Foundation (National Nanoscience Infrastructure Network)

  • Nicki E Watson
  • Arthur A McClelland

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Franz-Ulrich Hartl, Max Planck Institute for Biochemistry, Germany

Version history

  1. Preprint posted: December 16, 2020 (view preprint)
  2. Received: January 11, 2022
  3. Accepted: June 13, 2022
  4. Accepted Manuscript published: June 20, 2022 (version 1)
  5. Version of Record published: June 30, 2022 (version 2)
  6. Version of Record updated: July 5, 2022 (version 3)

Copyright

© 2022, Serebryany et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Eugene Serebryany
  2. Sourav Chowdhury
  3. Christopher N Woods
  4. David C Thorn
  5. Nicki E Watson
  6. Arthur A McClelland
  7. Rachel E Klevit
  8. Eugene I Shakhnovich
(2022)
A native chemical chaperone in the human eye lens
eLife 11:e76923.
https://doi.org/10.7554/eLife.76923

Share this article

https://doi.org/10.7554/eLife.76923

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