Mir204 and Mir211 suppress synovial inflammation and proliferation in rheumatoid arthritis by targeting Ssrp1

  1. Qi-Shan Wang
  2. Kai-Jian Fan
  3. Hui Teng
  4. Sijia Chen
  5. Bing-Xin Xu
  6. Di Chen  Is a corresponding author
  7. Ting-Yu Wang  Is a corresponding author
  1. Department of Pharmacy, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, China
  2. Faculty of Pharmaceutical Sciences, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, China
11 figures, 3 tables and 1 additional file

Figures

Figure 1 with 1 supplement
Mir204/Mir211 downregulate in fibroblast-like synoviocyte (FLS) of collagen-induced arthritis (CIA) mice.

(A) Low expression levels of Mir204/Mir211 in FLS of CIA mice. (B) qRT-PCR analysis of the relative Mir204/Mir211 expression in CIA FLS 48 hr after transfection. Please see Figure 1—source data 1. …

Figure 1—source data 1

Numerical data obtained during experiments represent in Figure 1.

https://cdn.elifesciences.org/articles/78085/elife-78085-fig1-data1-v1.xlsx
Figure 1—figure supplement 1
Transfection efficiency of Mir204/Mir211 in CIA FLS and viability of CIA FLS with different treatments.

(A) Transfection efficiency of Mir204/Mir211 in collagen-induced arthritis (CIA) fibroblast-like synoviocyte (FLS) at different time points. To determine transfection efficiency, Mir204/Mir211

Effects of Mir204/Mir211 on apoptosis and cell migration ability of collagen-induced arthritis (CIA) fibroblast-like synoviocyte (FLS).

(A) Effects of Mir204/Mir211 on apoptosis of CIA FLS. (B) Effects of Mir204/Mir211 on cell migration ability of CIA FLS. Representative photomicrographs show a wound scratch assay at specific time …

Figure 2—source data 1

Numerical data obtained during experiments represent in Figure 2.

https://cdn.elifesciences.org/articles/78085/elife-78085-fig2-data1-v1.xlsx
Effects of Mir204/Mir211 on the inflammatory responses of collagen-induced arthritis fibroblast-like synoviocyte.

All data are displayed as a value relative to those in the NC group. Please see Figure 3—source data 1. Data are pooled from at least three independent experiments and are presented as mean ± SEM. …

Figure 3—source data 1

Numerical data obtained during experiments represent in Figure 3.

https://cdn.elifesciences.org/articles/78085/elife-78085-fig3-data1-v1.xlsx
Effects of Mir204/Mir211 on NF-κB signaling pathway.

(A) Western blot assays of the NF-κB pathway in transfected collagen-induced arthritis (CIA) fibroblast-like synoviocyte (FLS). (B) Immunofluorescence staining of p65 nuclear translocation in …

Figure 5 with 1 supplement
Effects of Mir204/Mir211 on proliferation of collagen-induced arthritis (CIA) fibroblast-like synoviocyte (FLS).

(A) CCK-8 analysis in four groups of transfected CIA FLS. (B) Mir204/Mir211 induce G0/G1 phase arrest of CIA FLS. (C) Expression levels of cell cycle regulatory molecule in transfected CIA FLS. (D) …

Figure 5—figure supplement 1
Representative images of β-galactosidase staining of primary wild-type (WT) fibroblast-like synoviocyte (FLS) and double knockout (dKO) FLS.

Primary FLSs are isolated from male WT and dKO C57 mice. FLSs are seeded in six-well plates at the density of 5×105, and β-galactosidase staining is performed.

Mir204/Mir211 target structure-specific recognition protein 1 (Ssrp1) in collagen-induced arthritis (CIA) fibroblast-like synoviocyte (FLS).

(A) Diagrams describing the process of predicting target genes of Mir204/Mir211 (B) Diagram of putative Mir204/Mir211 binding sequence in Ssrp1 3’ untranslated region (UTR) from TargetScan. (C) High …

Figure 7 with 1 supplement
Effects of Mir204/Mir211 on cell proliferation by targeting structure-specific recognition protein 1 (Ssrp1).

(A) Overexpression of Mir204/Mir211 decreases SSRP1 expression. (B) Mir204/Mir211 decrease Ki-67 levels by targeting Ssrp1. Please see Figure 7—source data 1. Data are pooled from four independent …

Figure 7—figure supplement 1
Overexpression of structure-specific recognition protein 1 (SSRP1) in 293T cells.

293T cells are divided into Ctrl group (untransfected), SSRP1 group (transfected with pcDNA3.1 - Ssrp1 plasmid), and negative control group (transfected with empty vector). 293T cells are …

Figure 8 with 1 supplement
High expression level of structure-specific recognition protein 1 (SSRP1) in collagen-induced arthritis (CIA) Mir204/Mir211 double knockout (dKO) mice.

(A) Severity of arthritis in CIA wild-type (WT) and CIA dKO mice. The arthritis scores, paw swelling, and body weight are documented by two independent blinded observers since the day of the second …

Figure 8—source data 1

Numerical data obtained during experiments represent in Figure 8.

https://cdn.elifesciences.org/articles/78085/elife-78085-fig8-data1-v1.xlsx
Figure 8—figure supplement 1
Representative images of tartrate resistant acid phosphatase (TRAP) and immunohistochemistry (IHC) staining of knee joints in collagen-induced arthritis (CIA) wild-type (WT) and CIA double knockout (dKO) mice.

Representative photographs of mouse knee joints with TRAP staining and IHC staining for matrix metalloproteinase (MMP)-13 are shown.

Figure 9 with 1 supplement
Structure-specific recognition protein 1 (Ssrp1) knockdown exerts anti-arthritis effect in collagen-induced arthritis (CIA) Mir204/Mir211 double knockout (dKO) mice.

(A) Ssrp1 knockdown ameliorates swelling degrees of hind paws in CIA Mir204/Mir211 dKO mice. Representative photomicrographs of knee joint sections in synovium and cartilage stained with hematoxylin …

Figure 9—source data 1

Numerical data obtained during experiments represent in Figure 9.

https://cdn.elifesciences.org/articles/78085/elife-78085-fig9-data1-v1.xlsx
Figure 9—figure supplement 1
Representative images of tartrate resistant acid phosphatase (TRAP) and immunohistochemistry (IHC) staining of knee joints in three groups of mice.

Representative photographs of mouse knee joints with TRAP and IHC staining for matrix metalloproteinase (MMP)-13 are shown.

Graphical summary of how Mir204/211 suppresses synovial inflammation and proliferation in rheumatoid arthritis (RA) by targeting structure-specific recognition protein 1 (Ssrp1).

Primary fibroblast-like synoviocytes (FLSs) derived from collagen-induced arthritis (CIA) mice display decreased expression of Mir204/211. On the one hand, upon being challenged by IL-1β, FLS from …

Author response image 1

Tables

Table 1
PCR primer sequences.
GenePrimer sequences
mmu-Mir204 forward primer5 '- GGGCTTCCCTTTGTCATCCTAT –3'
mmu-Mir211 forward primer5 ' - GGGCTTCCCTTTGTCATCCTT –3'
Universal U6 Forward primer5 ' - GCAAATTCGTGAAGCGTTCCATA –3'
Universal PCR reverse primer5 ' - AACGAGACGACGACAGAC –3'
Table 2
PCR primer sequences.
NameGeneForward primer sequenceReverse primer sequence
β-ActinActb5'-GTGACGTTGACATCCGTAAAGA-3'5'-GCCGGACTCATCGTACTCC-3'
IL-1βIl1b5'-GAAATGCCACCTTTTGACAGTG-3'5'-TGGATGCTCTCATCAGGACAG-3'
IL-4Il45'-GGTCTCAACCCCCAGCTAGT-3'5'-GCCGATGATCTCTCTCAAGTGAT-3'
IL-6Il65'-CTGCAAGAGACTTCCATCCAG-3'5'-AGTGGTATAGACAGGTCTGTTGG-3'
IL-8Il85'-TCGAGACCATTTACTGCAACAG-3'5'-CATTGCCGGTGGAAATTCCTT-3'
IL-10Il105'-GCTGGACAACATACTGCTAACC-3'5'-ATTTCCGATAAGGCTTGGCAA-3'
IL-23Il235'-CAGCAGCTCTCTCGGAATCTC-3'5'-TGGATACGGGGCACATTATTTTT-3'
TGF-β1Tgfb15'-CCACCTGCAAGACCATCGAC-3'5'-CTGGCGAGCCTTAGTTTGGAC-3'
Cyclin D1Ccnd15'-GCGTACCCTGACACCAATCTC-3'5'-ACTTGAAGTAAGATACGGAGGGC-3'
p16Cdkn2a5'-CGCAGGTTCTTGGTCACTGT-3'5'-TGTTCACGAAAGCCAGAGCG-3'
p21Cdkn1a5'-CCTGGTGATGTCCGACCTG-3'5'-CCATGAGCGCATCGCAATC-3'
p57Cdkn1c5'-GCAGGACGAGAATCAAGAGCA-3'5'-GCTTGGCGAAGAAGTCGTT-3'
SSRP1Ssrp15'-CAGAGACATTGGAGTTCAACGA-3'5'-GACGGCTCAATCGAAGCCTC-3'
Table 3
Target proteins detected by western blotting.
GeneProteinCompanyMolecular weight (kDa)Dilution ratio
RelaNF-κB p65Cell Signaling Technology651:1000
NfkbiaI-κBαCell Signaling Technology391:1000
ChukIKKαCell Signaling Technology851:1000
IkbkbIKKβCell Signaling Technology871:1000
Pik3r1PI3KCell Signaling Technology851:1000
Akt1P-AKTCell Signaling Technology601:1000
Akt1AKTCell Signaling Technology601:1000
Trp53P53Cell Signaling Technology531:1000
Ssrp1SSRP1Cell Signaling Technology811:1000
GapdhGAPDHCell Signaling Technology371:1000

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