Connectomic analysis of the Drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes
- Advanced Science Research Center, The City University of New York, United States
- Center for Theoretical Neuroscience, Zuckerman Institute, Columbia University, United States
- Department of Neuroscience and Behavior, Barnard College, United States
- Department of Electrical Engineering, Columbia University, United States
Figures
Figure 1
The circadian clock neuron network and identified clock neurons in the hemibrain.
(A) The circadian clock network is shown in both hemispheres. Currently identified cells are shown in color (s-LNvs red, l-LNvs light red, LNds in orange, DN1a in purple, a subset of DN1p in blue) …
Figure 2 with 1 supplement
Connectivity patterns of the s-LNvs.
(A–C). Synaptic connections of the four pdf + s LNvs, s-LNv_R_1 through s-LNv_R_4. Neuronal morphology is shown in gray. In A and B, inputs to the s-LNvs are shown in blue, outputs are shown in …
Figure 2—figure supplement 1
Connections of individual s-LNvs.
(A–D). Synaptic connections of each of the four pdf + s LNvs, s-LNv1 (A), s-LNv2 (B), s-LNv3 (C), and s-LNv4 (D), either to all neurons (left columns), to other clock cells except s-LNvs (middle …
Figure 3 with 2 supplements
Connectivity patterns of the LNds.
(A–C). Synaptic connections of the six LNds. Neuronal morphology is shown in gray. In A and B, inputs to the s-LNvs are shown in blue, outputs are shown in magenta. (A) All connections, including …
Figure 3—figure supplement 1
Connections of individual LNds.
(A–F). Synaptic connections of each of the six LNds, LNd1 (A), LNd2 (B), LNd3 (C), LNd4 (D), LNd5 (E), and LNd6 (F) either to all neurons (left columns), to other clock cells except LNds (middle …
Figure 3—figure supplement 2
Connections of E cell classes.
(A–C). Synaptic connections of the three E cell classes, E3a (A), E1 (B), and E2 (C) either to all neurons (left columns), to other clock cells except LNds (middle columns), or to other LNds (right …
Figure 4 with 2 supplements
M cells are homogeneous while E cells can be clustered in three distinct groups.
(A–B) Jaccard indices for overlap in synaptic partners of M and E cells. Only includes synaptic partners that make medium or strong connections. Higher index values indicate more similarity in …
Figure 4—figure supplement 1
Comparison of medium and strong inputs of 5th s-LNv relative to the pdf +s LNvs and the LNds.
(A–B) Supervenn diagrams indicate the number of shared inputs between all combinations of neurons, either among the 5th LNv and the LNds (A) or among the 5th LNv and the pdf +s LNvs (B). Each neuron …
Figure 4—figure supplement 2
Comparison of medium and strong outputs of 5th s-LNv relative to the pdf +s LNvs and the LNds.
(A–B) Supervenn diagrams indicate the number of shared outputs between all combinations of neurons, either among the 5th s-LNv and the LNds (A) or among the 5th LNv and the pdf + s LNvs (B). Each …
Figure 5
Neuropils innervated by M and E cells.
(A–B). Percentage of connections located in each of the indicated neuropils. Medium and strong connections are included. (A) Neuropils in which the outputs of each of the four pdf + s LNvs are …
Figure 6 with 2 supplements
Strong shared outputs of M cells.
(A–C) The six cells that are strong shared outputs of the four M cells involve three different neuronal types (SPL316-R, SLP403-R, and SMP232-R). The M cells outputs onto each representative neuron …
Figure 6—figure supplement 1
Connections of individual strong shared outputs of s-LNvs.
(A–F). Synaptic connections of each of the six cells that receive strong shared inputs from all four pdf +s LNvs. Each cell is indicated in gray. Inputs from clock neurons to each cell are indicated …
Figure 6—figure supplement 2
Neuroanatomy of the three SLP316 cells and identified DN1s.
(A) Five DN1ps (green) that exhibit contralateral as well as dorsal projections have been identified, and are referred to as DN1pA. (B) Two DN1ps (magenta) that lack a contralateral or dorsal …
Figure 7
Strong shared outputs of E1.
(A–C) The cells that are strong shared outputs of E1 involve multiple neuronal types. E1 outputs onto three representative neurons are shown. E1 neurons (LNd4 and LNd5) are shown in gray in the left …
Figure 8
Strong shared outputs of E2.
(A–C) The cells that are strong shared outputs of E2 involve multiple neuronal types. E2 outputs onto three representative neurons are shown. E2 neurons (LNd6 and the 5th s-LNv) are shown in gray in …
Figure 9
Strong shared outputs of E3.
(A–C) The cells that are strong shared outputs of E3 involve multiple neuronal types. E3 outputs onto three representative neurons are shown. E3 neurons (LNd1, LNd2, and LNd3) are shown in gray in …
Figure 10
Connections within the clock neuron network.
(A) Combined weights of medium and strong connections among different classes of identified clock neurons. Arrows indicate the direction of the connection. Some classes, such as E2, are strongly …
Tables
Table 1
Identification of Clock Neurons within the Hemibrain Volume.
| BodyId | Type | Sequential label | Subphase |
|---|---|---|---|
| 2068801704 | s-LNv | s-LNv1 | M |
| 1664980698 | s-LNv | s-LNv2 | M |
| 2007068523 | s-LNv | s-LNv3 | M |
| 1975347348 | s-LNv | s-LNv4 | M |
| 5813056917 | LNd | LNd4 | E1 |
| 5813021192 | LNd | LNd5 | E1 |
| 5813069648 | LNd | LNd6 | E2 |
| 511051477 | 5th LNv | 5th LNv | E2 |
| 296544364 | LNd | LNd1 | E3 |
| 448260940 | LNd | LNd2 | E3 |
| 5813064789 | LNd | LNd3 | E3 |
| 356818551 | LPN | LPN1 | |
| 480029788 | LPN | LPN2 | |
| 450034902 | LPN | LPN3 | |
| 546977514 | LPN | LPN4 | |
| 264083994 | DN1a | DN1a1 | |
| 5813022274 | DN1a | DN1a2 | |
| 5813010153 | DN1pA | DN1pA1 | |
| 324846570 | DN1pA | DN1pA2 | |
| 325529237 | DN1pA | DN1pA3 | |
| 387944118 | DN1pA | DN1pA4 | |
| 387166379 | DN1pA | DN1pA5 | |
| 386834269 | DN1pB | DN1pB1 | |
| 5813071319 | DN1pB | DN1pB2 | |
| 1884625521 | l-LNv | lLNv1 | |
| 2065745704 | l-LNv | lLNv2 | |
| 5813001741 | l-LNv | lLNv3 | |
| 5813026773 | l-LNv | lLNv4 |
Additional files
-
Supplementary file 1
Inputs onto clock neurons.
List of Body IDs of all neurons that provide inputs onto currently identified circadian clock neurons and the weight of those pre-synaptic connections.
- https://cdn.elifesciences.org/articles/79139/elife-79139-supp1-v3.xls
-
Supplementary file 2
Outputs of clock neurons.
List of Body IDs of all neurons that receive inputs from currently identified circadian clock neurons and the weight of those post-synaptic connections.
- https://cdn.elifesciences.org/articles/79139/elife-79139-supp2-v3.xls
-
MDAR checklist
- https://cdn.elifesciences.org/articles/79139/elife-79139-mdarchecklist1-v3.pdf
