Single-cell RNA sequencing analysis of shrimp immune cells identifies macrophage-like phagocytes
- Shantou University, China
- Genedenovo Biotechnology Company Limited, China
Abstract
Despite the importance of innate immunity in invertebrates, the diversity and function of innate immune cells in invertebrates are largely unknown. Using single-cell RNA-seq, we identified prohemocytes, monocytic hemocytes, and granulocytes as the three major cell-types in the white shrimp hemolymph. Our results identified a novel macrophage-like subset called monocytic hemocytes 2 (MH2) defined by the expression of certain marker genes, including Nlrp3 and Casp1. This subtype of shrimp hemocytes is phagocytic and expresses markers that indicate some conservation with mammalian macrophages. Combined, our work resolves the heterogenicity of hemocytes in a very economically important aquatic species and identifies a novel innate immune cell subset that is likely a critical player in the immune responses of shrimp to threatening infectious diseases affecting this industry.
Data availability
The sequence data reported in this paper have been deposited in the Genome Sequence Archive of the Beijing Institute of Genomics, Chinese Academy of Sciences, gsa.big.ac.cn (accession no. PRJCA006297). All other data are available in this manuscript and online in the Supplementary Material.
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Single-cell RNA sequencing for shrimp (Penaeus vannamei) hemocytes treated with recombinant CREGGenome Sequence Archive, RJCA006297.
Article and author information
Author details
Funding
National Natural Science Foundation of China (41976123)
- Fan Wang
Guangdong Science and Technology Department (14600703)
- Fan Wang
Li Ka Shing Foundation (2020LKSFG01E)
- Yueling Zhang
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Irene Salinas, University of New Mexico, United States
Ethics
Animal experimentation: All the animal-related experiments were in accordance with Shantou University guidelines.
Version history
- Received: May 9, 2022
- Preprint posted: May 18, 2022 (view preprint)
- Accepted: October 5, 2022
- Accepted Manuscript published: October 6, 2022 (version 1)
- Version of Record published: October 20, 2022 (version 2)
Copyright
© 2022, Yang et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Single-cell RNA sequencing analysis of shrimp immune cells identifies macrophage-like phagocytes
eLife 11:e80127.
https://doi.org/10.7554/eLife.80127
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