gene (Mus musculus) | Ccr4 | GenBank | Gene ID: 12773 | |
gene (Mus musculus) | Ccr7 | GenBank | Gene ID: 12775 | |
strain, strain background (Mus musculus) | C57BL6/J | Jackson Laboratory | Strain #: 000664 | |
genetic reagent (Mus musculus) | B6.SJL-Ptprca PepCb/BoyJ (CD45.1) | Jackson Laboratory | Strain #: 002014 | |
genetic reagent (Mus musculus) | C57BL/6-Tg(TcraTcrb)1100Mjb/J (OT-I) | Jackson Laboratory | Strain #: 003831 | |
genetic reagent (Mus musculus) | B6.Cg-Tg(TcraTcrb)425Cbn/J (OT-II) | Jackson Laboratory | Strain #: 004194 | |
genetic reagent (Mus musculus) | B6(Cg)-Rag2tm1.1Cgn/J (Rag2-/-) | Jackson Laboratory | Strain #: 008449 | |
genetic reagent (Mus musculus) | B6.129P2(C)Ccr7tm1Rfor/J (Ccr7-/-) | Jackson Laboratory | Strain #: 006621 | |
genetic reagent (Mus musculus) | B6.129S2-H2dlAb1-Ea/J (H2-/-) | Jackson Laboratory | Strain #: 003584 | |
genetic reagent (Mus musculus) | B6.129P2-B2mtm1Unc/DcrJ (B2m-/-) | Jackson Laboratory | Strain #: 002087 | |
genetic reagent (Mus musculus) | C57BL/6-Tg(Nr4a1-EGFP/cre)820Khog/J (Nr4a1GFP) | Jackson Laboratory | Strain #: 016617 | |
genetic reagent (Mus musculus) | Ccr4-/- | Chvatchko et al., 2000 | | Generously provided by A.D. Luster (Massachusetts General Hospital, Boston, MA) |
genetic reagent (Mus musculus) | pCX-EGFP | Wright et al., 2001 | | Generously provided by Irving L. Weissman (Stanford University, Stanford, CA) |
genetic reagent (Mus musculus) | Rag2p-GFP | Boursalian et al., 2004 | | Generously provided by Ellen R. Richie (the University of Texas MD Anderson Center, Houston, TX) |
genetic reagent (Mus musculus) | Ccr4-/-; Ccr7-/-(DKO) | This paper | | See Materials and Methods, Section “Mice” |
genetic reagent (Mus musculus) | OT-I Rag2-/- | This paper | | See Materials and Methods, Section “Mice” |
genetic reagent (Mus musculus) | OT-II Rag2-/- H2-/- | This paper | | See Materials and Methods, Section “Mice” |
antibody | PE/Cyanine 7 Anti-mouse CD3, Rat Monoclonal, clone 17 A2 | BioLegend | Cat # 100220; RRID:AB_1732057 | (1:200) |
antibody | PE Anti-mouse CCR4, Armenian Hamster Monoclonal, clone 2 G12 | BioLegend | Cat # 131204; RRID:AB_1236367 | (1:200) |
antibody | APC Anti-mouse CCR7, Rat Monoclonal, clone 4B12 | BioLegend | Cat # 120108; RRID:AB_389234 | (1:200) |
antibody | Brilliant Violet 510 Anti-mouse CD4, Rat Monoclonal, clone RM4-5 | BioLegend | Cat # 100559; RRID:AB_2562608 | (1:200) |
antibody | PE/Cyanine7 Anti-mouse CD5, Rat Monoclonal, clone 53–7.3 | BioLegend | Cat # 100622; RRID:AB_2562773 | (1:200) |
antibody | FITC Anti-mouse CD8a, Rat Monoclonal, clone 53–6.7 | BioLegend | Cat # 100706; RRID:AB_312745 | (1:200) |
antibody | Alexa Flour 700 Anti-mouse CD11b, Rat Monoclonal, clone M1/70 | BioLegend | Cat # 101222; RRID:AB_493705 | (1:200) |
antibody | Pacific Blue Anti-mouse CD11c, Armenian Hamster Monoclonal, clone N418 | BioLegend | Cat # 117322; RRID:AB_755988 | (1:200) |
antibody | PerCP/Cyanine5.5 Anti-mouse CD19, Rat Monoclonal, clone 1D3/CD19 | BioLegend | Cat # 152405; RRID:AB_2629814 | (1:200) |
antibody | APC Anti-mouse CD25, Rat Monoclonal, clone PC61 | BioLegend | Cat # 102012; RRID:AB_312861 | (1:200) |
antibody | APC Anti-mouse CD45.1, Mouse (A. SW) monoclonal, clone A20 | BioLegend | Cat # 110714; RRID:AB_313503 | (1:200) |
antibody | PE Anti-mouse CD45.2, Mouse (SJL) monoclonal, clone 104 | BioLegend | Cat # 109808; RRID:AB_313445 | (1:200) |
antibody | Biotin Anti-mouse CD69, Armenian Hamster monoclonal, clone H1.2F3 | BioLegend | Cat # 104504; RRID:AB_313107 | (1:200) |
antibody | Brilliant Violet 510 Anti-mouse CD90.2, Rat monoclonal, clone 30 H12 | BioLegend | Cat # 105335; RRID:AB_2566587 | (1:200) |
antibody | PE/Dazzle 594 Anti-mouse CXCR4, Rat monoclonal, clone L276F12 | BioLegend | Cat # 146514; RRID:AB_2563683 | (1:200) |
antibody | Alexa Flour 488 Anti-mouse Cleaved Caspase 3 (Asp175), Rabbit polyclonal | Cell Signaling Technology | Cat # 9669 S | (1:200) |
antibody | PE Anti-mouse EpCAM, Rat monoclonal, clone G8.8 | BioLegend | Cat # 118206; RRID:AB_1134172 | (1:200) |
antibody | APC/Cyanine7 Anti-mouse F4/80, Rat monoclonal, clone BM8 | BioLegend | Cat # 123118; RRID:AB_893477 | (1:200) |
antibody | PE/Cyanine5 Anti-mouse Gr1, Rat monoclonal, clone RB6-8C5 | BioLegend | Cat # 108410; RRID:AB_313375 | (1:200) |
antibody | PerCP/Cyanine5.5 Anti-mouse H-2Kb (MHCI), Mouse (BALB/c) monoclonal, clone AF6-88.5 | BioLegend | Cat # 116516; RRID:AB_1967133 | (1:200) |
antibody | PE/Cyanine7 Anti-mouse I-A/I-E (MHCII), Rat Monoclonal, clone M5/114.15.2 | BioLegend | Cat # 107630; RRID:AB_2069376 | (1:200) |
antibody | Alexa Flour 488 Anti-mouse PDCA1, Rat Monoclonal, clone 927 | BioLegend | Cat # 127012; RRID:AB_1953287 | (1:200) |
antibody | PE/Cyanine5 Anti-mouse Ter-119, Rat monoclonal, clone TER-119 | BioLegend | Cat # 116209; RRID:AB_313710 | (1:200) |
antibody | APC Anti-mouse Sirpα, Rat monoclonal, clone P84 | BioLegend | Cat # 144014; RRID:AB_2564061 | (1:200) |
antibody | Brilliant Violet 650Anti-mouse XCR1, mouse monoclonal, clone ZET | BioLegend | Cat # 148220; RRID:AB_2566410 | (1:200) |
antibody | Biotin Anti-mouse CD11c, Armenian Hamster monoclonal, clone N418 | BioLegend | Cat # 117303; RRID:AB_313772 | (1:50) |
antibody | APC Anti-mouse CD4, Rat monoclonal, clone GK1.5 | BioLegend | Cat # 100412; RRID:AB_312696 | (1:100) |
antibody | APC Anti-mouse CD31, Rat monoclonal, clone 390 | BioLegend | Cat# 102410; RRID:AB_312905 | (1:100) |
antibody | Biotin anti-mouse/human CD45R/B220, Rat monoclonal, clone RA3-6B2 | BioLegend | Cat # 103204; RRID:AB_312989 | (1:200) |
antibody | Purified Rat anti-mouse B220, Rat monoclonal, clone RA3.3A1/6.1 | BioXCell | Cat # BE0067 | (1:100) |
antibody | Purified Rat anti-mouse CD3, Rat monoclonal, clone 17 A2 | BioXCell | Cat # BE0002 | (1:50) |
antibody | Purified Rat anti-mouse CD8, Rat monoclonal, clone 53.6.72 | BioXCell | Cat # BE0004-1 | (1:100) |
antibody | Purified Rat anti-mouse CD25, Rat monoclonal, clone PC-61.5.3 | BioXCell | Cat # BE0012 | (1:100) |
antibody | Purified Rat anti-mouse CD11b, Rat monoclonal, clone M1/70 | BioXCell | Cat # BE0007 | (1:100) |
antibody | Purified Rat anti-mouse Gr-1, Rat monoclonal, clone RB6-8C5 | BioXCell | Cat # BE0075 | (1:100) |
antibody | Purified Rat anti-mouse Ter-119, Rat monoclonal, clone TER-119 | BioXCell | Cat # BE0183 | (1:100) |
antibody | Alexa Fluor 594 AffiniPure Donkey anti-mouse IgG, Donkey polyclonal | Jackson ImmunoResearch | Cat # 715585151; RRID:AB_2340855 | (1:100) |
antibody | Goat anti-mouse CCL22, Polyclonal, Goat polyclonal | R&D Systems | Cat # AF439; RRID:AB_355360 | (1.2 ug/mL) |
antibody | Normal Goat IgG control, Goat polyclonal | R&D Systems | Cat # AB-108-C; RRID:AB_354267 | (1:200) |
antibody | CD8a- Alexa Fluor 594, rat monoclonal, clone 53.6.7 | BioLegend | Cat# 100758 | (1:100) |
Other flow cytometry reagents | Qdot 605 Streptavidin Conjugate | Invitrogen | Cat # Q10101MP | (1:200) |
Other immunostaining reagents | Streptavidin-Alexa Fluor 488 | Invitrogen | Cat# S32354 | |
Other flow cytometry reagents | Ulex Europaeus Agglutinin I (UEAI), Biotinylated | Vector Laboratories | SKU B-1065–2 | (1:1000) |
sequence-based reagent | CCL17 Forward | Cowan et al., 2014 | qPCR Primer | 5’-AGTGGAGTGTTCCAGGGATG-3’ |
sequence-based reagent | CCL17 Reverse | Cowan et al., 2014 | qPCR Primer | 5’-CCAATCTGATGGCCTTCTTC-3’ |
sequence-based reagent | CCL19 Forward | Kurd and Robey, 2016 | qPCR Primer | 5’-GCTAATGATGCGGAAGACTG-3’ |
sequence-based reagent | CCL19 Reverse | Kurd and Robey, 2016 | qPCR Primer | 5’-ACTCACATCGACTCTCTAGG-3’ |
sequence-based reagent | CCL21 Forward | Seach et al., 2008 | qPCR Primer | 5’-GCAGTGATGGAGGGGGTCAG-3’ |
sequence-based reagent | CCL21 Reverse | Seach et al., 2008 | qPCR Primer | 5’-CGGGGTGAGAACAGGATTGC-3’ |
sequence-based reagent | CCL22 Forward | Hu et al., 2015b | qPCR Primer | 5’-AGGTCCCTATGGTGCCAATGT-3’ |
sequence-based reagent | CCL22 Reverse | Hu et al., 2015b | qPCR Primer | 5’- CGGCAGGATTTTGAGGTCCA-3’ |
sequence-based reagent | β-actin Forward | Camara et al., 2019 | qPCR Primer | 5’- CACTGTCGAGTCGCGTCCA-3’ |
sequence-based reagent | β-actin Reverse | Camara et al., 2019 | qPCR Primer | 5’- CATCCATGGCGAACTGGTGG-3’ |
peptide, recombinant protein | Recombinant Murine CCL17 | Peprotech | Cat # 250–43 | |
peptide, recombinant protein | Recombinant Murine CCL19 | Peprotech | Cat # 250-27B | |
peptide, recombinant protein | Recombinant Murine CCL21 | Peprotech | Cat # 250–13 | |
peptide, recombinant protein | Recombinant Murine CCL22 | Peprotech | Cat # 250–23 | |
peptide, recombinant protein | Recombinant Murine CXCL12 | Peprotech | Cat # 250-20B | |
peptide, recombinant protein | Recombinant Mouse CCL25 | R&D Systems | Cat # 481-TK-025 | |
commercial assay or kit | Fixation / Permeablization Kit (RUO) | BD | Cat # 554714 | |
commercial assay or kit | CellTrace Violet cell proliferation kit | Invitrogen | Cat # C34557 | |
commercial assay or kit | Celltracker Red CMTPX Dye | Invitrogen | Cat # C34552 | |
commercial assay or kit | eBioscience Indo-1 AM Calcium Sensor Dye | Invitrogen | Cat # 65-0856-39 | |
commercial assay or kit | TRIzol Reagent | Invitrogen | Cat # 15596026 | |
commercial assay or kit | SYBR Green PCR Master Mix | Applied Biosystems | Cat # 4309155 | |
commercial assay or kit | Mouse TLR1-9 Agonist Kit | Invivogen | Cat # tlrl-kit1mw | |
commercial assay or kit | Dynabeads sheep anti-rat IgG | Invitrogen | Cat # 11035 | |
commercial assay or kit | ProLong Gold Antifade Mountant | ThermoFisher | Cat # P36930 | |
software, algorithm | Flowjo v10.8.1 | BD | https://www.flowjo.com/ | |
software, algorithm | Graphpad PRISM v9.3.1 | Graphpad Software | https://www.graphpad.com/ | |
software, algorithm | Imaris v9.8.0 | Oxford Instruments | https://imaris.oxinst.com/ | |
software, algorithm | Biorender | Biorender | https://biorender.com/ | |
software, algorithm | ImageJ | NIH | https://imagej.nih.gov/ | |