Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish

  1. Yun Deng
  2. Haihong Wang
  3. Xiaohui Liu
  4. Hao Yuan
  5. Jin Xu
  6. Hugues de Thé
  7. Jun Zhou  Is a corresponding author
  8. Jun Zhu  Is a corresponding author
  1. Shanghai Jiao Tong University, China
  2. South China University of Technology, China
  3. Inserm, France

Abstract

Macrophages and their precursor cells, monocytes, are the first line of defense of the body against foreign pathogens and tissue damage. Although the origins of macrophages are diverse, some common transcription factors (such as PU.1) are required to ensure proper development of monocytes/macrophages. Here we report that the deficiency of zbtb14, a transcription repressor gene belonging to ZBTB family, leads to an aberrant expansion of monocyte/macrophage population in zebrafish. Mechanistically, Zbtb14 functions as a negative regulator of pu.1, and SUMOylation on a conserved lysine is essential for the repression activity of Zbtb14. Moreover, a serine to phenylalanine mutation found in an acute myeloid leukemia (AML) patient could target ZBTB14 protein to autophagic degradation. Hence, ZBTB14 is a newly identified gene implicated in both normal and malignant myelopoiesis.

Data availability

RNA sequencing dataset generated in this study was deposited with Dryad-https://doi.org/10.5061/dryad.9cnp5hqms.

The following data sets were generated
    1. Deng Y
    2. Wang H
    3. Liu X
    4. Yuan H
    5. Xu J
    6. de Thé H
    7. Zhou J
    8. Zhu J
    (2022) RNA SEQ
    Dryad Digital Repository, doi:10.5061/dryad.9cnp5hqms.

Article and author information

Author details

  1. Yun Deng

    Shanghai Institute of Hematology, Shanghai Jiao Tong University, Shanghai, China
    Competing interests
    The authors declare that no competing interests exist.
  2. Haihong Wang

    Shanghai Institute of Hematology, Shanghai Jiao Tong University, Shanghai, China
    Competing interests
    The authors declare that no competing interests exist.
  3. Xiaohui Liu

    Shanghai Institute of Hematology, Shanghai Jiao Tong University, Shanghai, China
    Competing interests
    The authors declare that no competing interests exist.
  4. Hao Yuan

    Shanghai Institute of Hematology, Shanghai Jiao Tong University, Shanghai, China
    Competing interests
    The authors declare that no competing interests exist.
  5. Jin Xu

    Division of Cell, Developmental and Integrative Biology, South China University of Technology, Guangzhou, China
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-6840-1359
  6. Hugues de Thé

    UMR 1050, Inserm, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
  7. Jun Zhou

    Shanghai Institute of Hematology, Shanghai Jiao Tong University, Shanghai, China
    For correspondence
    zj10802@rjh.com.cn
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-0472-3188
  8. Jun Zhu

    CNRS-LIA Hematology and Cancer, Shanghai Jiao Tong University, Shanghai, China
    For correspondence
    zhuj1966@yahoo.com
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-7983-3130

Funding

National Natural Science Foundation of China (NO.32171097)

  • Jun Zhou

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: Ethics StatementThe study was approved by the Ethics Committee of Rui Jin Hospital Affiliated toShanghai Jiao Tong University School of Medicine. Zebrafish experimental procedures were conducted in accordance with the protocols approved by the Institutional Animal Care and Use Committee (IACUC) of Shanghai Jiao Tong University (2020-3#).

Reviewing Editor

  1. Florent Ginhoux, Agency for Science Technology and Research, Singapore

Version history

  1. Received: June 3, 2022
  2. Preprint posted: July 4, 2022 (view preprint)
  3. Accepted: October 6, 2022
  4. Accepted Manuscript published: October 7, 2022 (version 1)
  5. Version of Record published: October 14, 2022 (version 2)
  6. Version of Record updated: October 18, 2022 (version 3)

Copyright

© 2022, Deng et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Yun Deng
  2. Haihong Wang
  3. Xiaohui Liu
  4. Hao Yuan
  5. Jin Xu
  6. Hugues de Thé
  7. Jun Zhou
  8. Jun Zhu
(2022)
Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish
eLife 11:e80760.
https://doi.org/10.7554/eLife.80760

Share this article

https://doi.org/10.7554/eLife.80760

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