Desiccation resistance differences in Drosophila species can be largely explained by variations in cuticular hydrocarbons
- Michigan State University, United States
- University of Florida, United States
Abstract
Maintaining water balance is a universal challenge for organisms living in terrestrial environments, especially for insects, which have essential roles in our ecosystem. Although the high surface area to volume ratio in insects makes them vulnerable to water loss, insects have evolved different levels of desiccation resistance to adapt to diverse environments. To withstand desiccation, insects use a lipid layer called cuticular hydrocarbons (CHCs) to reduce water evaporation from the body surface. It has long been hypothesized that the waterproofing capability of this CHC layer, which can confer different levels of desiccation resistance, depends on its chemical composition. However, it is unknown which CHC components are important contributors to desiccation resistance and how these components can determine differences in desiccation resistance. In this study, we used machine learning algorithms, correlation analyses, and synthetic CHCs to investigate how different CHC components affect desiccation resistance in 50 Drosophila and related species. We showed that desiccation resistance differences across these species can be largely explained by variation in CHC composition. In particular, length variation in a subset of CHCs, the methyl-branched CHCs (mbCHCs), is a key determinant of desiccation resistance. There is also a significant correlation between the evolution of longer mbCHCs and higher desiccation resistance in these species. Given that CHCs are almost ubiquitous in insects, we suggest that evolutionary changes in insect CHC components can be a general mechanism for the evolution of desiccation resistance and adaptation to diverse and changing environments.
Data availability
All data generated or analyzed during this study are included in the manuscript and supporting source data file. Code used is uploaded as source code 1-3.
Article and author information
Author details
Funding
National Science Foundation (2054773)
- Henry Chung
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Lynn M. Riddiford
Version history
- Received: June 7, 2022
- Preprint posted: June 28, 2022 (view preprint)
- Accepted: December 5, 2022
- Accepted Manuscript published: December 6, 2022 (version 1)
- Version of Record published: December 16, 2022 (version 2)
Copyright
© 2022, Wang et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Desiccation resistance differences in Drosophila species can be largely explained by variations in cuticular hydrocarbons
eLife 11:e80859.
https://doi.org/10.7554/eLife.80859
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