Sugar sensation and mechanosensation in the egg-laying preference shift of Drosophila suzukii
- Department of Molecular, Cellular and Developmental Biology, Yale University, United States
Figures
Figure 1 with 1 supplement
Taste contributes to the difference in egg-laying preference between D. suzukii and D. melanogaster.
(A) Two-choice egg-laying paradigm. Female flies (n=4–10 flies per plate) whose olfactory organs had been removed were allowed to lay eggs in the dark. The preference index was calculated as (#eggs …
Figure 1—figure supplement 1
D. suzukii has an egg-laying preference for ripe strawberries and for ripe strawberry purée, while D. melanogaster prefers overripe strawberries and overripe strawberry purée.
(A) Given a choice between whole, intact ripe or overripe organic strawberries obtained commercially, D. suzukii and D. melanogaster in cages showed opposite preferences. n=7–9. (B) Given a choice …
Figure 2 with 1 supplement
D. suzukii shows a weaker egg-laying preference than D. melanogaster for sweeter substrates.
(A) The single-fly egg-laying preference paradigm. The preference index is calculated as (#eggs in higher sugar concentration - #eggs in lower sugar concentration)/total #eggs. (B–D) Preference …
Figure 2—figure supplement 1
Preferences for high-sugar concentrations are not exclusively due to a preference for high osmolarity.
The egg-laying preference for sucrose vs. sorbitol, a sugar alcohol considered tasteless to the fly. The preferences are the same as between sucrose and plain agarose (taken from Figure 2B). Each …
Figure 3 with 1 supplement
Electrophysiological responses to sugars.
All sugars were tested at 100 mM concentrations. (A) Taste sensilla of the labellum. Figure 3A is reproduced from Figure 3D by Dweck et al., 2021. (B) Sample traces from the indicated sensilla. (C–K)…
Figure 3—figure supplement 1
Dose dependence of sucrose responses.
(A) Sample traces of the L8 sensillum to the indicated sucrose concentrations in each species. (B) Dose dependence of the firing frequency of the L8 sensillum of each species. ns, not significant. …
Figure 4 with 1 supplement
Reduced expression of taste receptor genes in the D. suzukii labellum and leg.
(A) Principal component analysis of the labellar and leg transcriptomes of D. melanogaster and D. suzukii. (B) Integrated genomics viewer (IGV) browser view of the aligned reads of the nine sugar Gr …
Figure 4—figure supplement 1
Gustatory receptor (Gr), ionotropic receptor (Ir), and odorant binding protein (Obp) expression in tibial and tarsal leg segments of D. melanogaster.
Shown are leg expression levels of Grs (A), Irs (B), and Obps (C) that have average FPKM (fragments per kilobase of feature per million mapped reads) values ≥0.5.
Figure 5
Trehalose elicits reduced egg-laying preference and egg-laying in D. suzukii.
(A) Two-choice egg-laying preferences. n=18–20 plates, each with a single fly. Each egg-laying preference index is compared to 0 using the Wilcoxon signed-rank test. The Mann-Whitney test is used to …
Figure 6
D. suzukii prefers harder substrates than D. melanogaster and expresses higher levels of mechanosensory channels in taste organs.
(A) Numbers of eggs laid in a no-choice egg-laying paradigm on substrates of the indicated agarose concentrations. n=18–20. One-way ANOVA followed by Tukey’s multiple comparison test. Values …
Figure 7
D. suzukii and D. melanogaster respond differently to combinations of sweetness and stiffness.
(A) The egg-laying preferences of D. suzukii and D. melanogaster for substrates with the same sweetness but different stiffness. (B) The egg-laying preferences for substrates with the same stiffness …
Additional files
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Supplementary file 1
FPKM values for D. melanogaster leg RNAseq.
- https://cdn.elifesciences.org/articles/81703/elife-81703-supp1-v1.xlsx
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Supplementary file 2
TPM values for all RNAseq samples of D. melanogaster and D. suzukii.
- https://cdn.elifesciences.org/articles/81703/elife-81703-supp2-v1.xlsx
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Supplementary file 3
DESeq2 differential gene expression analysis of leg RNAseq between D. suzukii and D. melanogaster.
- https://cdn.elifesciences.org/articles/81703/elife-81703-supp3-v1.xlsx
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Supplementary file 4
DESeq2 differential gene expression analysis of labellum RNAseq between D. suzukii and D. melanogaster.
- https://cdn.elifesciences.org/articles/81703/elife-81703-supp4-v1.xlsx
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MDAR checklist
- https://cdn.elifesciences.org/articles/81703/elife-81703-mdarchecklist1-v1.docx
