Molecular characterization of the intact mouse muscle spindle using a multi-omics approach
- Weizmann Institute of Science, Israel
- Max Delbrueck Center for Molecular Medicine, Germany
- University of Aberdeen, United Kingdom
- Durham University, United Kingdom
Abstract
The proprioceptive system is essential for the control of coordinated movement, posture and skeletal integrity. The sense of proprioception is produced in the brain using peripheral sensory input from receptors such as the muscle spindle, which detects changes in the length of skeletal muscles. Despite its importance, the molecular composition of the muscle spindle is largely unknown. In this study, we generated comprehensive transcriptomic and proteomic datasets of the entire muscle spindle isolated from the murine deep masseter muscle. We then associated differentially expressed genes with the various tissues composing the spindle using bioinformatic analysis. Immunostaining verified these predictions, thus establishing new markers for the different spindle tissues. Utilizing these markers, we identified the differentiation stages the spindle capsule cells undergo during development. Together, these findings provide comprehensive molecular characterization of the intact spindle as well as new tools to study its development and function in health and disease.
Data availability
Sequencing data have been deposited in GEO under accession number GSE208147.The raw data of proteomic profiling were deposited in the ProteomeXchange via the Proteomic Identification Database (PRIDE partner repository)
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Molecular characterization of the intact mouse muscle spindle using a multi-omics approachNCBI Gene Expression Omnibus, GSE208147.
Article and author information
Author details
Funding
The David and Fela Shapell Family Center for Genetic Disorders Research
- Elazar Zelzer
The Julie and Eric Borman Family Research Funds
- Elazar Zelzer
The Nella and Leon Benoziyo Center for Neurological Diseases
- Elazar Zelzer
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Benjamin L Prosser, University of Pennsylvania Perelman School of Medicine, United States
Ethics
Animal experimentation: All experiments involving mice were approved by the Institutional Animal Care and Use Committee (IACUC) of the Weizmann Institute (#02180222-2).
Version history
- Received: July 13, 2022
- Preprint posted: July 14, 2022 (view preprint)
- Accepted: February 3, 2023
- Accepted Manuscript published: February 6, 2023 (version 1)
- Version of Record published: February 15, 2023 (version 2)
Copyright
© 2023, Bornstein et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Molecular characterization of the intact mouse muscle spindle using a multi-omics approach
eLife 12:e81843.
https://doi.org/10.7554/eLife.81843
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