Molecular and anatomical characterization of parabrachial neurons and their axonal projections
- Howard Hughes Medical Institute, University of Washington, United States
- University of Washington, United States
Abstract
The parabrachial nucleus (PBN) is a major hub that receives sensory information from both internal and external environments. Specific populations of PBN neurons are involved in behaviors including food and water intake, nociceptive responses, breathing regulation, as well as learning and responding appropriately to threatening stimuli. However, it is unclear how many PBN neuron populations exist and how different behaviors may be encoded by unique signaling molecules or receptors. Here we provide a repository of data on the molecular identity, spatial location, and projection patterns of dozens of PBN neuron subclusters. Using single-cell RNA sequencing, we identified 21 subclusters of neurons in the PBN and neighboring regions. Multiplexed in situ hybridization showed many of these subclusters are enriched within specific PBN subregions with scattered cells in several other regions. We also provide detailed visualization of the axonal projections from 21 Cre-driver lines of mice. These results are all publicly available for download and provide a foundation for further interrogation of PBN functions and connections.
Data availability
Raw and preprocessed data for scRNA-seq: NCBI GEO accession number GSE207708Code for analysis of scRNA-Seq data: https://github.com/stuberlab/Pauli-Chen-Basiri-et-al-2022Raw and normalized data for RiboTag: NCBI GEO accession number GSE207153Images from RNAscope and all tracing experiments: Zenodo DOI: 10.5281/zenodo.6707404; https://doi.org/10.5281/zenodo.6707404
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Molecular and Anatomical Characterization of Parabrachial Neurons and Their Axonal ProjectionsNCBI Gene Expression Omnibus, GSE207708.
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Gene expression profiling of Calca neurons in the parabrachial nucleus (PBN)NCBI Gene Expression Omnibus, GSE207153.
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Molecular and Anatomical Characterization of Parabrachial Neurons and Their Axonal ProjectionsZenodo, DOI 10.5281/zenodo.6707404.
Article and author information
Author details
Richard D Palmiter
Funding
National Institutes of Health (R01-DA24908)
- Richard D Palmiter
National Institutes of Health (R01-DA032750)
- Garret D Stuber
National Institutes of Health (R01-DA038168)
- Garret D Stuber
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: All experiments were approved by the Institutional Animals Care and Use Committee at the University of Washington (Protocol #2183-02).
Copyright
© 2022, Pauli et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Molecular and anatomical characterization of parabrachial neurons and their axonal projections
eLife 11:e81868.
https://doi.org/10.7554/eLife.81868
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