(A-C) Immunofluorescence of cortical neurons in the compartmentalized device using the indicated antibodies (A) Collage of a compartmentalized culture stained for MCT8 (magenta). (B–C) MCT8 staining in magenta, Lamp1 in green; colocalization is indicated as white. (B) In the MC-CS and (C) in the MC-AS. (D) MCT8-immunoreactivity (silver grains) was present in the outer cell membrane of neuronal elements, and in the membrane of vesicles of axonal profiles (arrows and arrowheads, respectively), on the nuclear membrane (inset in E), in vesicles close to the nucleus of the neurons (E), and the trans and cis Golgi apparatus (F). (G) Detail of the squared area in (F, H). T3I125 was applied in the MC-AS. (I). After 72 hr, T3I125 was detected in the MC-CS medium (no T3I125 was detected after 24 hr; inset in the blue panel). (J). Effect of 2 µM SC on T3 uptake and transport. The size of the T3I125 peak in the MC-CS decreased after 72 hr compared to (I, K). Quantitation of T3I125 transported to the MC-CS medium under the indicated conditions. The Y-axis in % T3I125 in the MC-CS medium vs. T3I125 added to the MC-AS medium. Values are mean ± SD of 4–5 independent experiments; *p<0.05 in comparison with T3I125 incubation. SC, Silychristin; XH, Xanthohumol; Ax, axon; Nu, nucleus. Scale bars are 25 µM on A-E and G, 150 µM on F, 500 nm on J, K, and M, and 200 nm on L.