Firing patterns of ventral hippocampal neurons predict the exploration of anxiogenic locations
- Division of Cognitive Neurobiology, Center for Brain Research, Medical University of Vienna, Austria
- Laboratory of Systems Neuroscience, Department of Physiology, University of Bern, Switzerland
Figures
Figure 1 with 1 supplement
The activity of ventral hippocampal neurons is dynamically modulated during elevated plus maze (EPM) exploration.
(A) Top, picture of the EPM. Bottom, the percentage of the time spent in different areas by the rats (n=6) during the exploration of the EPM. The time spent in closed arms is significantly higher …
Figure 1—figure supplement 1
Nissl-stained sections and peak densities.
(A) Nissl-stained sections of four different brains showing the tetrode tracks. Red arrows indicate the tip end and electric lesion (see Materials and methods). (B) Density plots of peak firing from …
Figure 2 with 1 supplement
Removal of protective sidewalls along an elevated linear maze (ELM) induces anxiety behaviour.
(A). ELM configurations with sidewalls along the entire track (closed-closed [CC], both arms closed, left), and with sidewalls removed for half of the track (closed-open [CO], called one arm closed …
Figure 2—figure supplement 1
Analysis of runs along the elevated linear maze (ELM) for different configurations.
(A) Boxplots of the number of trials per session for each configuration. (B) Boxplots of the number of rewarded (Rew., green) or not rewarded trials (N.Rew., white) per session for each …
Figure 3 with 3 supplements
Overrepresentation and remapping of ventral hippocampal activity during anxiety.
(A) Z-transformed firing rates (colour coded) of ventral hippocampal neurons during the exploration of the elevated linear maze (ELM) and sorted by the spatial location of their peak firing activity …
Figure 3—figure supplement 1
Neuronal activity during elevated linear maze (ELM) exploration.
(A) Activity of two individual vCA1 hippocampal neurons during the ELM exploration. For each neuron: top, configurations, closed-closed (CC) (shown by a rectangle with full lines) and a closed-open …
Figure 3—figure supplement 2
Spike-shape analyses.
(A) Left, histogram of the correlation between the averaged spike shapes of the first third and last third of the recording. Note that 100% of the neurons showed higher correlation than 0.98, …
Figure 3—figure supplement 3
Remapping proportions for different subdivisions.
(A) Same comparisons as in Figure 3B and C but only taking into account putative pyramidal cells. No significant differences were observed for the distribution of peak firing rates when expected …
Figure 4 with 1 supplement
Spatial properties of neuronal activity during elevated linear maze (ELM) exploration.
(A) Top, paired comparison of the spatial properties for PiO (firing peak in open area of the closed-open [CO] configuration) neurons between their activity in the closed-closed (CC) and the CO …
Figure 4—figure supplement 1
Spatial and firing – properties of recorded neurons.
(A) Histograms of the spatial properties of neurons for different configurations (top: spatial information, middle: sparsity, bottom: coherence). (B) Top panels, spatial properties comparison …
Figure 5 with 1 supplement
The direction-dependent activity of vCA1 neurons is homogenised after exposure to an anxiogenic location.
(A) Neuronal activity of individual ventral hippocampus (vH) neurons while animals explored the elevated linear maze (ELM) in both the closed-closed (CC) and the closed-open (CO) configuration. Blue …
Figure 5—figure supplement 1
Additional analysis on the direction-dependent activity of ventral hippocampus (vH) neurons.
(A) Same comparisons as in Figure 5B and C but only for putative pyramidal neurons. Place field similarity (PFS) index was higher during the closed-open (CO) configuration (cyan) compared to the …
Figure 6 with 2 supplements
The activity of ventral hippocampal neurons predicts the extent of exploration of an anxiogenic location.
(A) For two behavioural sessions, the furthest spatial bin visited on each trial is plotted. The dotted line indicates the spatial bin set as a criterion to define two different types of …
Figure 6—figure supplement 1
Speed comparison and neuronal activity of individual cells.
(A) Raster plots of the spike activity in time for the neuronal pairs in Figure 6A. Each tick indicates a spike. Each row shows a trial during which the animal goes from the closed towards the …
Figure 6—figure supplement 2
Further analysis on support vector machine (SVM) predictions.
(A) Expanded plot as in Figure 6D. Black boxes indicate the shuffled SVM performance minus the actual performance of the best neuronal pair in the session. Red boxes show the distribution of the …
