(A) rILN→DS neurons project ipsilaterally. Coronal mouse brain section through the rILN. Following AAVrg-tdTomato injection in the left DS left rILN→DS neurons labeled with tdTomato. (B) Left: schematic of experimental strategy to label rILN→DS neurons. Right: a patched neuron (GFP, injected with AlexaFluor[AF]–488) among tdTomato-labeled rILN neurons. (C) Representative traces showing central medial (CM), paracentral (PC), and central lateral (CL) neuronal responses to 500 ms current injection steps. (D) Top: neuronal membrane capacitance of rILN neurons mapped across three rostral-caudal coronal planes in light to dark color gradient spanning minimum to maximum capacitance values. Bottom: membrane capacitance did not differ between CM (n=17), PC (n=12), and CL (n=13) nuclei. (E–I) Analysis of membrane resistance (E), input resistance (F), resting membrane potential (G), action potential (AP) threshold (H), and maximum firing rate elicited by current steps (I; CM n=16), for which no significant differences existed between the three rILN nuclei. Scale bars: 500 µm (A), 100 µm (B), 20 mV and 200 ms (C). One-way ANOVA (D–I). n=number of cells. Filled and open data points represent cells from male and female mice, respectively. Error bars = standard error of the mean (SEM).