3D display of Ntn1 (A) and Slc6a3 (B, dopamine marker) from the Allen Brain Atlas. (C–D) 20 X magnification images of in situ hybridization (RNAScope) for Ntn1 (green) and Slc32a1 (GABA marker; red, …
Cell counts.
(A–B) Schematics summarizing cell type-specific knockout procedure. (A) Adult mice were injected bilaterally into the VTA with AAV-FLEX-SaCas9-HA-sgNtn1 and AAV-FLEX-YFP. Control mice received an …
Cell counts.
(A) Schematic of DAT-Cre dopamine specific Ntn1cKO. (B) Sample traces from control (top panel) and DAT Ntn1 cKO mice (bottom panel). (C–D) mEPSC amplitude (C) and frequency (D) measured from …
EPSCs and IPSCs from targeted cells.
Additional EPSC and IPSC data from non-targeted cells.
(A) mIPSC amplitude and frequency of DAT-Cre fluorescently identified dopamine neurons (n=20 controls, n=27 cKO, t=0.8283, df = 45 p>0.05 and t=0.6668, df = 45 p>0.05). (B) mIPSC amplitude and …
(A) Miniature excitatory postsynaptic current (mEPSC) amplitude and frequency recorded from non-fluorescent cells in DAT-Cre mice (presumptively non-dopamine neurons) (n=16 controls, n=11 cKO, …
Analysis of 1/CV2 from miniature excitatory postsynaptic current (mEPSC) data in (A) DAT-Cre mice measured from fluorescently identified dopamine neurons (n=35 controls, n=33 cKO, t=1.252, df = 66. …
(A) Schematic summarizing cell type-specific knockout procedure. (B) Distance traveled in 15 min bins over the course of three nights and two days (n=21 control; n=15 cKO, Two-way ANOVA, Group F(1, …
Behavioral data for Figure 4.
(A) Average ambulatory activity (beam breaks) averaged across three nights and two days (n=21 control; n=18 cKO, Night: t=0.3938, df = 37, p=0.6960, Day: t=1.271, df = 37, p=0.2117). (B) Distance …
Behavioral data for Figure 4—figure supplement 1.
(A) Schematic summarizing cell type-specific knockout procedure. (B) Distance traveled in 15 min bins over the course of three nights and two days (n=26 controls, n=23 cKO, Two-way ANOVA Group F(1, …
Behavioral data for Figure 5.
(A) Average ambulatory activity (beam breaks) averaged across three nights and two days (n=26 control; n=26 cKO, t=3.109, df = 50 **p=0.01 and t=3.227, df = 50 **p=0.01). (B) Distance traveled …
Behavioral data for Figure 5—figure supplement 1.
(A) Model of Ntn1 loss of function in the ventral tegmental area (VTA) on excitatory and inhibitory balance. (B) Schematic of GABA and Dopamine Ntn1 cKO. (C) Distance traveled in 15 min bins over …
Behavioral data for Figure 6.
(A) Ambulatory activity (beam breaks) averaged across three nights and two days (n=26 controls n=21 cKO, Night: t=1.010, df = 45, p=0.3178 and Day: t=2.091, df = 45 *p<0.05). (B) Distance traveled …
Behavioral data for Figure 6—figure supplement 1.