Mating activates neuroendocrine pathways signaling hunger in Drosophila females
- University of California, Berkeley, United States
Abstract
Mated females reallocate resources to offspring production, causing changes to nutritional requirements and challenges to energy homeostasis. Although observed across species, the neural and endocrine mechanisms that regulate the nutritional needs of mated females are not well understood. Here, we find that mated Drosophila melanogaster females increase sugar intake, which is regulated by the activity of sexually dimorphic insulin receptor (Lgr3) neurons. In virgins, Lgr3+ cells have reduced activity as they receive inhibitory input from active, female specific pCd-2 cells, restricting sugar intake. During copulation, males deposit sex peptide into the female reproductive tract, which silences a three-tier mating status circuit and initiates the female postmating response. We show that pCd-2 neurons also become silenced after mating due to the direct synaptic input from the mating status circuit. Thus, in mated females pCd-2 inhibition is attenuated, activating downstream Lgr3+ neurons and promoting sugar intake. Together, this circuit transforms the mated signal into a long-term hunger signal. Our results demonstrate that the mating circuit alters nutrient sensing centers to increase feeding in mated females, providing a mechanism to increase intake in anticipation of the energetic costs associated with reproduction.
Data availability
All data generated and analyzed during this study are included in the manuscript and supporting files
Article and author information
Author details
Gabriella R Sterne
Funding
National Institutes of Health (R01DC013280)
- Kristin Scott
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Sonia Sen, Tata Institute for Genetics and Society, India
Version history
- Preprint posted: October 21, 2022 (view preprint)
- Received: November 23, 2022
- Accepted: May 13, 2023
- Accepted Manuscript published: May 15, 2023 (version 1)
- Version of Record published: May 30, 2023 (version 2)
Copyright
© 2023, Laturney et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Mating activates neuroendocrine pathways signaling hunger in Drosophila females
eLife 12:e85117.
https://doi.org/10.7554/eLife.85117
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