Nested circuits mediate the decision to vocalize
- Department of Neurobiology, Duke University, United States
Figures
Figure 1 with 4 supplements
AmgC/M-PAG neurons are active in response to threatening stimuli.
(A) (Left) Schematic shows the behavioral paradigm: in the neutral condition, a stimulus female mouse along with a dish containing clean bedding was placed into the home cage of either a male or a …
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Figure 1—source data 1
Source data for Figure 1, Figure 1—figure supplements 1 and 2.
- https://cdn.elifesciences.org/articles/85547/elife-85547-fig1-data1-v2.xlsx
Figure 1—figure supplement 1
AmgC/M-PAG neurons are the most strongly activated by threatening stimuli.
(A) Mean peak AmgC/M-PAG activity in male and female mice as the animal approached and investigated a dish containing either 2MT, a control odor (ethyl tiglate) or a novel plastic “toy” (left; N=7 …
Figure 1—figure supplement 2
AmgC/M-PAG neurons respond to various stimuli.
(A) Comparison of mean dF/F (%) one second before behavior onset and mean peak dF/F (%) during behavior of interest during social interactions (chemoinvestigation of same-sex conspecific, …
Figure 1—video 1
Robust social vocalizations in the presence of neutral odor.
A resident male mouse produced robust USVs when pursuing a novel female in the presence of a dish containing clean bedding in his home cage. Video is shown at the top, with a synchronized …
Figure 1—video 2
Predator odor (2MT) suppresses social vocalizations.
The same resident male mouse shown in Figure 1—video 1 produced significantly fewer USVs to a novel female in the presence of a dish containing bedding with 2MT odor in his home cage. Video is shown …
Figure 2 with 1 supplement
Activation of AmgC/M-PAG neurons suppresses vocalizations in female mice.
(A) (Top) Behavioral paradigm for inducing USV production from female mice. (Bottom) Isolated female mice had similar numbers of USVs produced per minute, number of USV bouts produced per minute, …
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Figure 2—source data 1
Source data for Figure 2.
- https://cdn.elifesciences.org/articles/85547/elife-85547-fig2-data1-v2.xlsx
Figure 2—video 1
Optogenetic activation of AmgC/M-PAG neurons transiently suppresses ultrasonic vocalizations (USVs) in a female mouse.
A previously isolated female resident mouse that expressed ChR2 in AmgC/M-PAG neurons is shown following and producing USVs to a female intruder. Optogenetic activation of AmgC/M-PAG neurons …
Figure 3 with 1 supplement
Activation of Esr1 +AmgCC/M neurons suppresses vocalizations.
(A) In situ hybridization data for Esr1 mRNA in wild-type male mouse. (Allen Institute for Brain Science. Allen Mouse Brain Atlas, available from: http://mouse.brain-map.org/experiment/show/79591677)…
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Figure 3—source data 1
Source data for Figure 3.
- https://cdn.elifesciences.org/articles/85547/elife-85547-fig3-data1-v2.xlsx
Figure 3—video 1
Optogenetic activation of Esr1+ AmgCC/M neurons transiently suppresses ultrasonic vocalizations (USVs).
A resident male mouse that expressed ChR2 in Esr1+ AmgC/M neurons is shown following and producing USVs to a female intruder. Optogenetic activation of Esr1+ AmgC/M neurons transiently suppresses …
Figure 4
Monosynaptic inputs to AmgC/M-PAG neurons in male mice.
(A) (Left) Viral strategy shown for transsynaptic labeling of direct inputs to AmgC/M-PAG neurons (performed in N=4 males). (Right) Confocal images are shown of starter AmgC/M-PAG neurons. (B–E) …
Figure 5
POA inputs to the AmgC/M are active during affiliative social encounters and USV production in male mice.
(A) Viral strategy for photometry recording of Esr1+ POA terminal activity in AmgC/M region (POAEsr1-Amg terminal activity). (B) Average POAEsr1-Amg activity in an example male mouse (left) and mean …
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Figure 5—source data 1
Source data for Figure 5.
- https://cdn.elifesciences.org/articles/85547/elife-85547-fig5-data1-v2.xlsx
Figure 6 with 2 supplements
POAAmg cells are Esr1+, project to the PAG, promote USV production, and make inhibitory synapses onto AmgC/M-PAG neurons.
(A) Viral strategy for labeling AmgC/M-projecting POA neurons. (B) (Left) Confocal images showing GFP-labelled AmgC/M-projecting POA neurons and Esr1 staining. Gray arrow points to an example Esr1- …
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Figure 6—source data 1
Source data for Figure 6.
- https://cdn.elifesciences.org/articles/85547/elife-85547-fig6-data1-v2.xlsx
Figure 6—figure supplement 1
Cannula placement and ChR2 expression in POAAmg neurons.
(A) Confocal images show histology for ChR2 expression in POAAmg cells and cannula placement (N=4 males).
Figure 6—video 1
Optogenetic activation of POAAmg neurons elicits ultrasonic vocalizations (USVs).
The video shows an isolated male mouse that expressed ChR2 in POAAmg neurons. Optogenetic stimulation of these neurons elicits USVs. Video is shown at the top, with synchronized spectrogram and …
Figure 7
Model of nested circuits that mediate the decision to vocalize.
Inhibitory neurons within the AmgC/M encode threatening stimuli and suppress vocalizations via their direct inhibitory input to PAG-USV neurons, which gate USV production via their excitatory input …
