Homeostatic activation of aryl hydrocarbon receptor by dietary ligands dampens cutaneous allergic responses by controlling Langerhans cells migration
- Institut Curie, PSL Research University, INSERM, U932, France
- Institut Curie, PSL Research University, Plateforme de Pathologie Expérimentale, France
- Institut Curie, PSL Research University, Plateforme de Métabolomique et Lipidomique, France
Figures
Figure 1 with 1 supplement
Lack of dietary aryl hydrocarbon receptor (AhR) ligands exacerbates cutaneous allergic type 2 responses.
Mice were placed on AhR-poor diet or enriched in indole-3-carbinol (I3C diet) for 3 wk of adaptation prior to the start of experiments. (A–D) Papain or vehicle (PBS) was injected in the footpad at …
Figure 1—figure supplement 1
Comparable cutaneous allergic Th2 responses in mice fed with normal chow and indole-3-carbinol (I3C) diet.
Mice were placed on aryl hydrocarbon receptor (AhR)-poor or I3C diet for 3 wk of adaptation prior to the start of experiments. (A) Relative abundance of I3C was measured in the serum at day 0. …
Figure 2 with 1 supplement
Lack of dietary aryl hydrocarbon receptor (AhR) ligands does not impact airway allergic type 2 inflammation.
Mice were placed on AhR-poor or indole-3-carbinol (I3C) diet for 3 wk of adaptation prior to the start of experiments. Mice were exposed to papain or vehicle (PBS) intranasally four times at days 0, …
Figure 2—figure supplement 1
Gating strategy for bronchoalveolar cells.
Bronchoalveolar cells were gated on live singlets. One representative example for mice treated with vehicle (A) or papain (B).
Figure 3
Lack of dietary aryl hydrocarbon receptor (AhR) ligands worsens airway allergy after skin sensitization.
Mice were placed on AhR-poor or indole-3-carbinol (I3C) diet for 3 wk of adaptation prior to the start of experiments. Papain or vehicle (PBS) was injected in the footpad at day 0. At day 7, all …
Figure 4 with 1 supplement
Lack of dietary aryl hydrocarbon receptor (AhR) ligands impairs Langerhans cells migration, thereby increasing Th2 responses.
(A, B) Mice were placed on AhR-poor or indole-3-carbinol (I3C) diet for 3 wk of adaptation prior to the start of experiments. (A) Density of epidermal Langerhans cells was assessed by imaging Cd207(L…
Figure 4—figure supplement 1
Analysis of cutaneous allergy.
Mice were fed on aryl hydrocarbon receptor (AhR)-poor or indole-3-carbinol (I3C) diet for 3 wk of adaptation prior to the start of experiments. (A) Gating strategy for epidermal Langerhans cells. …
Figure 5 with 3 supplements
Dietary aryl hydrocarbon receptor (AhR) ligands regulate the inflammatory profile of epidermal cells.
Mice were placed on AhR-poor or indole-3-carbinol (I3C) diet for 3 wk of adaptation prior to the start of experiments. Papain or vehicle (PBS) was injected in the footpad at day 0. (A–D) After 6 hr, …
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Figure 5—source data 1
List of differentially expressed genes between conditions.
- https://cdn.elifesciences.org/articles/86413/elife-86413-fig5-data1-v2.xlsx
Figure 5—figure supplement 1
Dietary aryl hydrocarbon receptor (AhR) ligands modulate the transcriptomic profile of epidermal cells.
Mice were fed on AhR-poor or indole-3-carbinol (I3C) diet for 3 wk of adaptation prior to the start of experiments. Papain or vehicle (PBS) was injected in the footpad at day 0. After 6 hr, …
Figure 5—figure supplement 2
Diet-derived aryl hydrocarbon receptor (AhR) ligands do not impact keratinocyte barrier genes.
(A, B) RNA-seq data of the epidermis of germ-free or specific pathogen-free (SPF) mice (n = 8) was extracted from public source (GSE162925). Gene set enrichment analysis was performed for Gene …
Figure 5—figure supplement 3
Diet-derived aryl hydrocarbon receptor (AhR) ligands do not impact the structure of the skin barrier.
Mice were placed on AhR-poor diet or enriched in indole-3-carbinol (I3C diet) for 3 wk of adaptation prior to the start of experiments. (A) Papain or vehicle (PBS) was injected in the footpad at day …
Figure 6
Aryl hydrocarbon receptor (AhR) activation regulates keratinocyte production of TGF-β in mouse and human.
(A, B) Mice were placed on AhR-poor or indole-3-carbinol (I3C) diet for 3 wk of adaptation prior to the start of experiments. Papain or vehicle (PBS) was injected in the footpad at day 0. (A) After …
Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (mouse) | Cd207(Langerin)-eGFP-DTR | Kissenpfennig et al., 2005 | RRID:IMSR_JAX:016940 | Founder animals were donated by M.Vocanson (CIRI, Lyon, France) |
| Cell line (human) | HaCaT | Wilson, 2014 | RRID:CVCL_0038 | |
| Antibody | Anti-TCRβ BUV737 (hamster monoclonal, clone H57-597) | BD Biosciences | AB_2740818 | 1:200 |
| Antibody | Anti-CD11c PerCpCy5.5 (hamster monoclonal, clone HL3) | BD Biosciences | AB_1727422 | 1:200 |
| Antibody | Anti-SiglecF BV480 (rat monoclonal, clone E50-2440) | BD Biosciences | AB_2743940 | 1:200 |
| Antibody | Anti-CD45 FITC (rat monoclonal, clone 30-F11) | BioLegend | AB_312973 | 1:200 |
| Antibody | Anti-CD11b Pe-CF594 (rat monoclonal, clone M1/70) | BD Biosciences | AB_11154422 | 1:400 |
| Antibody | Anti-MHCII BV786 (rat monoclonal, clone M5/114.15.2) | BioLegend | AB_2565977 | 1:200 |
| Antibody | Anti-Ly6G BV605 (rat monoclonal, clone 1A8) | BioLegend | AB_2565880 | 1:200 |
| Antibody | Anti-CD4 BV650 (rat monoclonal, clone RM4-5) | BioLegend | AB_2562529 | 1:200 |
| Antibody | Anti-Ly6C AF700 (rat monoclonal, clone HK1.4) | BioLegend | AB_10643270 | 1:300 |
| Antibody | Anti-EpCAM APCFire750 (rat monoclonal, clone G8.8) | BioLegend | AB_2629758 | 1:400 |
| Antibody | Anti-CD11b PerCPCy5.5 (rat monoclonal, clone M1/70) | BD Biosciences | AB_394002 | 1:400 |
| Antibody | Anti-TCRβ APC (hamster monoclonal, clone H57-597) | BioLegend | AB_313435 | 1:200 |
| Antibody | Anti-CD4 FITC (rat monoclonal, clone RM4-5) | BD Biosciences | AB_394582 | 1:200 |
| Antibody | Anti-CD19 APC-Cy7 (rat monoclonal, clone 1D3) | BD Biosciences | AB_396770 | 1:200 |
| Antibody | Anti-CD172a BUV737 (rat monoclonal, clone P84) | BD Biosciences | AB_2871154 | 1:200 |
| Antibody | Anti-CD19 BV480 (rat monoclonal, clone 1D3) | BD Biosciences | AB_2739509 | 1:200 |
| Antibody | Anti-CD3 BV480 (hamster monoclonal, clone 500A2) | BD Biosciences | AB_2744035 | 1:200 |
| Antibody | Anti-XCR1 BV510 (rat monoclonal, clone ZET) | BioLegend | AB_2565231 | 1:100 |
| Antibody | Anti-CD11c BV785 (hamster monoclonal, clone N418) | BioLegend | AB_2565268 | 1:100 |
| Antibody | Anti-CD86 FITC (rat monoclonal, clone GL1) | BD Biosciences | AB_394993 | 1:100 |
| Antibody | Anti-CD26 PE (rat monoclonal, clone H194-112) | BioLegend | AB_2293047 | 1:200 |
| Antibody | Anti-CD40 PerCP-efluor710 (rat monoclonal, clone 1C10) | eBioscience | AB_2573677 | 1:100 |
| Antibody | Anti-PDL2 APC (rat monoclonal, clone TY25) | BioLegend | AB_2566345 | 1:100 |
| Antibody | Anti-MHC II BV650 (rat monoclonal, clone M5/114.15.2) | BioLegend | AB_2565975 | 1:400 |
| Software, algorithm | DESeq2 | Love et al., 2014 | v1.22.2 | |
| Software, algorithm | GSEA | Subramanian et al., 2005 | v4.0.3 | |
| Software, algorithm | QuPath | Bankhead et al., 2017 | v.0.3.1 | |
| Software, algorithm | FlowJo | FlowJo LLC | v10 |
