(A) Scoring matrix of top 20 scoring compounds present in both CMAP and the Prestwick library screens (basal agonists, Bas.Ag.; insulin sensitiser, Ins. Sens; insulin resistance reversers, Ins. Res. Rev). (B, C) Insulin stimulated GLUT4 translocation to the plasma membrane (PM) (B) and 2-deoxyglucose uptake (C) in control and insulin resistant L6 myotubes (palmitate) treated with thiostrepton or vehicle control. (D) Insulin-stimulated 2-deoxyglucose uptake in soleus and extensor digitorum longus (EDL) muscles from chow and Western diet (WD)-fed C57BL/6J and BXH9/TyJ following ex vivo treatment with thiostrepton or vehicle control. Data are mean with individual data points shown, n = 3–4 (B, C), n = 3–5 (BXH9); n = 11–14 (C57BL/6J). Significance was determined by one-way ANOVA with Student’s post hoc test. ** indicates significant difference from control (basal or chow-fed C57BL6/J) group (p<0.01), * indicates significant difference from control (p<0.05). # indicates significant difference from palmitate-treated or WD-fed control group (p<0.05).