Multiple dynamic interactions from basal ganglia direct and indirect pathways mediate action selection

  1. Hao Li
  2. Xin Jin  Is a corresponding author
  1. Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, United States
  2. Center for Motor Control and Disease, Key Laboratory of Brain Functional Genomics, East China Normal University, China
  3. NYU–ECNU Institute of Brain and Cognitive Science, New York University Shanghai, China
7 figures, 1 video, 1 table and 1 additional file

Figures

Figure 1 with 3 supplements
The neuronal dynamics in substantia nigra pars reticulata (SNr) during the 2–8 s action selection task.

(A) Schematic diagram for the design of 2–8 s task. (B) Correct rate for wild-type mice across 14 days’ training (n=10 mice, one-way repeated-measures ANOVA, significant effect of training days, F13,…

Figure 1—figure supplement 1
Behavioral performance across 14 days of training and the substantia nigra pars reticulata (SNr) neuronal recording on day 1.

(A) Lever press latency after lever extension for wild-type mice across 14 days’ training (n=10 mice, one-way repeated-measures ANOVA, effect of training days, F13,117=21.32, p<0.0001). (B) Lever …

Figure 1—figure supplement 2
Examples of substantia nigra pars reticulata (SNr) neuron and spiny projection neuron (SPN) subtypes.

(A–D) Firing activities of example SNr neurons of Type 1 (A), Type 2 (B), Type 3 (C), and Type 4 (D) in correct 8 s trials after 14 days training. Top panels: Raster plot of spikes across trials …

Figure 1—figure supplement 3
Substantia nigra pars reticulata (SNr) neuron activities in left and right hemisphere.

(A) Firing rate index (FRI) of neuronal activity for all task-related SNr neurons in correct 8 s trials recorded in the left hemisphere. The magnitude of FRI is color coded and the SNr neurons are …

Figure 2 with 1 supplement
Substantia nigra pars reticulata (SNr) neuronal dynamics reflect action selection but not interval time or reward value.

(A) Task diagram of 2–8 s control task with 10% 16 s probe trials. (B) Percentage of behavioral choice in 2 s, 8 s, and 16 s trials (blue: left choice; red: right choice) (n=9 mice, paired t-test, p<…

Figure 2—figure supplement 1
Behavioral statistics and neuronal dynamics of substantia nigra pars reticulata (SNr) neurons in the standard and reversed 2–8 s tasks.

(A) Correct rates of the same group of mice both in the standard and reversed 2–8 s tasks (n=6 mice, paired t-test, p=0.33). (B) Lever press ratios of the same group of mice both in the standard and …

Figure 3 with 2 supplements
Neuronal activity of striatal D1- and D2-expressing spiny projection neurons (D1- and D2-SPNs) during action selection.

(A) Firing rate index (FRI) of neuronal activity for all task-related SPNs in correct 8 s trials. SPNs were classified as Types 1–4. (B–E) Averaged FRI for Type 1 (B), Type 2 (C), Type 3 (D), Type 4 …

Figure 3—figure supplement 1
Striatum neuronal recording on day 1 of training, recording array, and optic fiber placement validation.

(A) Averaged FRI for Type 1, Type 2, Type 3, and Type 4 of spiny projection neurons (SPNs) in correct (red) and incorrect 2 s trials (gray). (B) Firing rate index (FRI) of neuronal activity for all …

Figure 3—figure supplement 2
Striatal projection neuron activities in left and right hemisphere.

(A) Firing rate index (FRI) of neuronal activity for all task-related spiny projection neurons (SPNs) in correct 8 s trials recorded in the left hemisphere. The magnitude of FRI is color coded and …

Figure 4 with 1 supplement
Selective genetic knockout and ablation of D1- or D2-expressing spiny projection neurons (D1- or D2-SPNs) distinctly alters action selection.

(A) Correct rate of control (n=11 mice) and D1-NR1 KO mice (n=16) in 2–8 s task during 14 days’ training (two-way repeated-measures ANOVA, significant difference between control and KO mice, F1,25=10…

Figure 4—figure supplement 1
Simulation of lesion experiments in Go/No-Go, Co-activation, and combination models.

(A) Diagram of Go/No-Go model. (B) The psychometric curves of behavior outputs simulated by Go/No-Go model in control (black) and D1-expressing spiny projection neurons (D1-SPNs) ablation condition …

Figure 5 with 1 supplement
Optogenetic manipulation of D1- vs. D2-expressing spiny projection neurons (D1- vs. D2-SPNs) differently regulates action selection.

(A) Schematic of optic fiber implantation for experimentally optogenetic excitation or inhibition of D1- or D2-SPNs in the dorsal striatum. (B, C) Schematic for optogenetic excitation (B) and …

Figure 5—figure supplement 1
Simulation of optogenetic manipulation in Go/No-Go and Co-activation models.

(A, B) Simulating optogenetic activation (A) and inhibition (B) of D1-expressing spiny projection neurons (D1-SPNs) at 2 s. Blue bar above indicates optogenetic activation. Yellow bar above …

Figure 6 with 3 supplements
A Triple-control computational model of basal ganglia direct and indirect pathways for action selection.

(A) Network structure of the cortico-basal ganglia model based on realistic anatomy and synaptic connectivity. Dashed lines indicate multi-synaptic connections. (B) Schematic of …

Figure 6—figure supplement 1
The neuronal activities in the ‘Triple-control’ model and simulation of lesion experiments.

(A) The simulated neuronal dynamics quantified as firing rate index (FRI) for the cortical neurons in 8 s trials. (B) The simulated neuronal dynamics quantified as FRI for the D1-expressing spiny …

Figure 6—figure supplement 2
Optogenetic activation of D1- vs. D2-expressing spiny projection neurons (D1- vs. D2-SPNs) differently regulates substantia nigra pars reticulata (SNr) activities in model and experiments.

(A) A computational motif of indirect pathway with collateral inhibitory synapse D2-SPN 1→ D2-SPN 2. The collateral synapse between D2-SPNs exhibits short-term depression. (B) Relationship between …

Figure 6—figure supplement 3
Computational modeling of optogenetic manipulation reveals that D1- vs. D2-expressing spiny projection neurons (D1- vs. D2-SPNs) differently regulates substantia nigra pars reticulata (SNr) outputs in the ‘Triple-control’ model.

(A, B) Schematic for optogenetic manipulation of D1-SPNs (A) and D2-SPNs (B) in the ‘Triple-control’ model. (C) Modeling of neuronal dynamics of SNr Type 1/Type 2 (left panel) and net output (right …

Figure 7 with 3 supplements
Computational modeling reveals direct and indirect pathways regulating action selection in a distinct manner.

(A) Schematic for manipulation of D1-expressing spiny projection neurons (D1-SPNs) in ‘Triple-control’ model. (B) Schematic of manipulation of D1-SPNs in the center-surround-context receptive field …

Figure 7—figure supplement 1
Computational modeling of manipulation reveals that Go/No-Go and Co-activation model differently predicts the behavioral outcomes.

(A) Diagram of Go/No-Go model. (B) Diagram of Co-activation model. (C) Correct rate change in 2 s (left panel) and 8 s trials (right panel) when manipulating D1-expressing spiny projection neurons …

Figure 7—figure supplement 2
Computational modeling reveals that the linear and nonlinear modulation of action selection by direct versus indirect pathway qualitatively hold with additional striatal collateral connections.

(A) Schematic for ‘Triple-control’ model with D1-D1 collateral connections. (B) Correct rate change in 2 s trials (upper panel) and 8 s trials (bottom panel) when manipulating D1-expressing spiny …

Figure 7—figure supplement 3
Computational modeling of dopaminergic modulation in the ‘Triple-control’ model.

(A) Diagram of Triple-control model with dopaminergic modulation on spiny projection neurons (SPNs). (B) Schematic of center-surround-context receptive field diagram with dopaminergic modulation …

Videos

Video 1
Stereotypical behavior of a well-trained mouse in a successful 8 s trial.

Tables

Key resources table
Reagent type (species) or resourceDesignationSource or referenceIdentifiersAdditional information
Strain, strain background (adeno-associated virus)AAV9-FLEX-DTR-GFPSalk GT3 CoreN/A
Strain, strain background (adeno-associated virus)AAV5-EF1a-DIO-hChR2(H134R)-mCherryUniversity of North Carolina
Vector Core
N/A
Strain, strain background (adeno-associated virus)AAV9-EF1a-DIO-hChR2(H134R)-eYFPUniversity of Pennsylvania
Vector Core
Cat# AV-9-20298P
Strain, strain background (adeno-associated virus)AAV5-EF1a-DIO-eNpHR3.0-eYFPUniversity of North Carolina
Vector Core
N/A
Chemical compound, drugMuscimol, GABAA receptor agonistSigma-AldrichCat# M1523
Chemical compound, drugDiphtheria toxinList Biological LabsPart# 150
Strain, strain background (Mus musculus)Mouse: C57BL/6Envigo/HarlanCode: 044
Strain, strain background (Mus musculus)Mouse: NR1f/f (B6.129S4-Grin1tm2Stl/J)Jackson LaboratoryStock# 005246
Strain, strain background (Mus musculus)Mouse: Ai32 (B6;129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J)Jackson LaboratoryStock# 012569
Strain, strain background (Mus musculus)Mouse: D1-cre (B6.FVB(Cg)-Tg(Drd1a-cre)EY217Gsat/Mmucd)MMRRCRRID: MMRRC_034258-UCD
Strain, strain background (Mus musculus)Mouse: A2a-cre (B6.FVB(Cg)-Tg(Adora2a-cre)KG139Gsat/Mmucd)MMRRCRRID: MMRRC_036158-UCD
Software, algorithmGraphPad PrismGraphPad SoftwareVersion 7.03https://www.graphpad.com/scientific-software/prism/
Software, algorithmMATLABMathWorksR2013ahttps://www.mathworks.com/products/matlab.html
Software, algorithmMed-PCMed AssociatesCat# SOF-735https://med-associates.com/product/med-pc-v/
Software, algorithmOffline SorterPlexonVersion 3.3.3https://plexon.com/products/offline-sorter/
Software, algorithmOmniPlexPlexonVersion 1.4.5https://plexon.com/products/omniplex-software/
Software, algorithmEthoVisionNoldusVersion 8.5
OtherMed Associates operant chamberMed AssociatesCat# MED-307W-D1
OtherElectrode ArrayInnovative NeurophysiologyN/A
Other473 nm laserLaserGlow TechnologiesN/A
Other532 nm laserLaserGlow TechnologiesN/A

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