Main text

Bhat D, Hauf S, Plessy C, Yokobayashi Y, Pigolotti S. 2022. Speed variations of bacterial replisomes. eLife 11:e75884. doi: 10.7554/eLife.75884.

Published 25 July 2022

We noticed a few minor errors after publication.

The first error is related with Eq. (9) of the paper. In deriving Eq. (9), we assumed that the dynamics of the two replisomes is symmetric, so that pst(x1,x2)=pst(x2,x1) . This assumption holds for all the models considered in the paper. However, we have not explicitly stated that Eq. (9) is derived under this assumption. Moreover, we realized that one can not identify each of the two integrals in Eq. (9) with the contribution coming from one of the two replisomes.

Corrected equation and following sentence:

(9) P(y)=kβ+k[|y|Ldx10Lx1dx2 pst(x1,x2)+L|y|Ldx20Lx2dx1 pst(x1,x2)yinanincompletegenome]+βk+βyinacompletegenome ,

where we assumed that the dynamics of the two replisomes is symmetric, so that pst(x1,x2)=pst(x2,x1), and we used that a randomly chosen genome is complete with probability (1-NS/N)=β/(k+β), see Equation 5.

Original equation and following text:

(9) P(y)=kβ+k[|y|Ldx10Lx1dx2 pst(x1,x2)ycopiedby1streplisome+L|y|Ldx20Lx2dx1 pst(x1,x2)ycopiedby2ndreplisome]+βk+βyinacompletegenome .

where the two terms in square brackets reflect the fact that either of the two replisomes can in principle have copied position y, and we used that a randomly chosen genome is complete with probability (1-NS/N)=β/(k+β), see Equation 5.

The second error regards the sentence in the discussion: “Similarly, oscillations in the mutation rate are also less evident at lower temperature and disappear in minimal medium (Niccum et al. 2019).” The first part of this statement is incorrect: Niccum et al. 2019 did observe evident oscillations at low temperature. We remark that their observation is not in disagreement with our hypothesis that the variations in replication speed and mutation rate stem from a common cause. In fact, the lowest temperature studied in (Niccum et al. 2019) is T=22 C. At this temperature, we observe pronounced oscillations in replisome speed as well (see Figure 3 in the eLife paper).

Removed sentence:

Similarly, oscillations in the mutation rate are also less evident at lower temperature and disappear in minimal medium (Niccum et al. 2019).

The third correction is related with the library preparation for sequencing. In the “Sequencing” subsection of the Methods section, the name of the kit used for library preparation was not accurate.

Corrected sentence:

Libraries were then prepared using the PCR-free NEBNext Ultra II DNA Library Prep Kit for Illumina.

Original sentence:

Libraries were prepared using the Illumina DNA PCR-Free Library Prep Kit.

The article has been corrected accordingly.

Article and author information

Author details

  1. Charles Plessy

  2. Yohei Yokobayashi

Version history

  1. Version of Record published: April 19, 2023 (version 1)

Copyright

© 2023, Bhat et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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  1. Deepak Bhat
  2. Samuel Hauf
  3. Charles Plessy
  4. Yohei Yokobayashi
  5. Simone Pigolotti
(2023)
Correction: Speed variations of bacterial replisomes
eLife 12:e88685.
https://doi.org/10.7554/eLife.88685

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