Multisensory gaze stabilization in response to subchronic alteration of vestibular type I hair cells
- Université Paris Cité, CNRS UMR 8002, INCC - Integrative Neuroscience and Cognition Center, France
- Departament de Ciències Fisiològiques, Universitat de Barcelona, Spain
- Institut de Neurociènces, Universitat de Barcelona, Spain
- Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), Spain
- Department of Paediatric Otolaryngology, Hôpital Necker-Enfants Malades, France
Figures
Figure 1 with 1 supplement
Effects of subchronic IDPN on canal- and otolithic- dependent VOR.
(A) Illustration of the angular horizontal vestibulo-ocular reflex (aVOR) set-up. All tests are performed in complete dark. (B) Examples of velocity aVOR traces in response to table rotations (1 Hz …
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Figure 1—source data 1
Effects of subchronic IDPN on canal- and otolithic- dependent VOR.
- https://cdn.elifesciences.org/articles/88819/elife-88819-fig1-data1-v2.xlsx
Figure 1—figure supplement 1
Effects of subchronic IDPN on canalar- and otolith-dependent VOR.
(A, B) aVOR (A) Gain and (B) Phase across all frequencies and weeks tested for IDPN (n=21) and SHAM (n=22) mice. The grey symbols in the left panel correspond to aVOR gain values associated with VAF …
Figure 2
Comparison of the dynamics of canalar and otolithic loss of function.
(A) Evolution of the Mean Gain of the aVOR (1 Hz) and OCR responses of IDPN mice (n=13) during the treatment and washout periods. (B) Individual gains for 1 Hz aVOR (left) and OCR (right) of the 13 …
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Figure 2—source data 1
Comparison of the dynamics of canalar and otolithic loss of function.
- https://cdn.elifesciences.org/articles/88819/elife-88819-fig2-data1-v2.xlsx
Figure 3 with 1 supplement
Immuno-labelling of HC in the central regions of the horizontal SCC ampulla and striolar region of the utricule Macula.
(A, C) Immunolabelling of type I HC (Spp1 + and CASPR1+), type II HC (Calre +), or all HC (Myo7a) for the SHAM, IDPN W6 and IDPN W12 groups in the central ampulla of the horizontal canal (A1) and …
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Figure 3—source data 1
Immuno-labelling of HC in the central regions of the horizontal SCC ampulla and striolar region of the utricule Macula.
- https://cdn.elifesciences.org/articles/88819/elife-88819-fig3-data1-v2.xlsx
Figure 3—figure supplement 1
Effects of the IDPN on the number of HC in the peripheral regions of the horizontal SCC ampulla and extrastriolar utricule Macula.
(A) Individual cell count of peripheral horizontal semi-circular canal type I and type II HC for W6 (1) andW12 (2). (B) Cell count of peripheral utricular type I and type II HC for W6 (1) and W12 …
Figure 4 with 1 supplement
Effects of subchronic IDPN treatment on the OKR.
(A) Illustration of the optokinetic reflex (OKR) set-up. (B) Example of raw traces of an OKR recorded in response to stimulation at 0.5 Hz at a peak velocity of 10°/s before (W0), after 4 weeks of …
Figure 4—figure supplement 1
Effect of the IDPN on optokinetic reflex amplitude and timing.
(A) OKR Gain across all frequencies and weeks tested (B) OKR Phase across all frequencies and weeks tested. (repeated measures ANOVA, *p<0.05; **p<0.01; ***p<0.001). Error bars represent ± SEM.
Figure 5
Comparison of the IDPN treatment on OKR and aVOR.
(A) Mean Δ aVOR and Δ Gain OKR for 0.2, 0.5, and 1 Hz for IDPN (n=12) and SHAM (n=12) mice. (B) Individual ΔOKR gains as a function of individual ΔaVOR gains. The 50% confidence interval of each …
Figure 6
Visuo-Vestibular interactions following IDPN treatment.
(A) aVOR, OKR, CGR data (CGR) and predicted CGR (MODEL) gains (left panel), and CGR phase (data and model, right panel), for the SHAM mice (n=12) at W6 and W12. (B) aVOR, OKR, CGR data (CGR) and …
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Figure 6—source data 1
Visuo-Vestibular interactions following IDPN treatment.
- https://cdn.elifesciences.org/articles/88819/elife-88819-fig6-data1-v2.xlsx
Tables
Table 1
Statistics table of the aVOR gain for the IDPN-treated group.
| W0 | W2 | W4 | W6 | W8 | W10 | W12 | |
|---|---|---|---|---|---|---|---|
| ns | *** | *** | *** | *** | *** | W0 | |
| ns | *** | *** | *** | *** | *** | W2 | |
| *** | *** | *** | *** | ns | ns | W4 | |
| *** | *** | *** | * | *** | *** | W6 | |
| *** | *** | *** | * | *** | *** | W8 | |
| *** | *** | ns | *** | *** | ns | W10 | |
| *** | *** | ns | *** | *** | ns | W12 |
Table 2
Statistics table of the OKR gain for the IDPN-treated group.
| W0 | W2 | W4 | W6 | W8 | W10 | W12 | |
|---|---|---|---|---|---|---|---|
| ns | ns | ns | *** | ns | ns | W0 | |
| ns | ns | ns | *** | ** | ns | W2 | |
| ns | ns | ns | ns | ns | ns | W4 | |
| ns | ns | ns | * | ns | ns | W6 | |
| *** | *** | ns | * | ns | ns | W8 | |
| ns | ** | ns | ns | ns | ns | W10 | |
| ns | ns | ns | ns | ns | ns | W12 |
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-Myosin 740 VIIa (rabbit polyclonal) | Proteus Biosciences | Cat#:25–6790 | 1/400 |
| Antibody | Anti-rabbit IgG H+L (donkey polyclonal) | Jackson Immuno Research | RRID:AB_2340616 | 1/500 |
| Antibody | Anti-contactin-associated protein (mouse monoclonal) | Neuromab | Cat#:75–001 | 1/400 |
| Antibody | Anti-mouse IgG H+L (donkey polyclonal) | Life Technologies | RRID:AB_141607 | 1/500 |
| Antibody | Anti-calretinin (guinea-pig polyclonal) | Synaptic Systems | Cat#: 214 104 | 1/500 |
| Antibody | Anti-guinea-pig IgG H+L (donkey polyclonal) | Jackson Immuno Research | RRID:AB_2340476 | 1/500 |
| Antibody | Anti-osteopontin (goat polyclonal) | R&D Systems | Cat#:AF808 | 1/400 |
| Antibody | Anti-goat IgG H+L (donkey polyclonal) | Invitrogen | RRID:AB_2535853 | 1/500 |
| Software | Spike2 | Cambridge Electronic Design | RRID:SCR_000903 | |
| Software | Prism | GraphPad | RRID:SCR_002798 | |
| Software | ImageJ | National Institutes of Health | RRID:SCR_003070 |
