(A) Genome browser image showing increased NRPE1 enrichment in h1 over heterochromatic (long, H3K9me2 enriched) and not euchromatic (short, not H3K9me2 enriched) transposable elements (TEs). (B) … see more
(A) Scatter plot showing H1 enrichment in wild-type (WT) vs. nrpe1 at euchromatic vs heterochromatic transposable elements (TEs). (B) Metaplot showing H1 enrichment at NRPE1 peaks in WT vs. nrpe1 (C)… see more
(A) Metaplot of DNase I accessibility in wild-typee (WT) vs h1 over euchromatic vs heterochromatic transposable elements (TEs). Data is obtained from Choi et al., 2020. (B) Scatterplot showing … see more
(A) Western blot of H1 and NRPE1, using same antibodies as used for ChIPseq. H1 is only detectable in the wild-type (WT) samples, not h1 mutant. (B) H1 ChIP-seq metaplots with over WT H1 peaks with … see more
(A) Chromosomal view of DNA methylation levels (average of 10 kb windows) in the genotypes indicated on chromosome 1. Y-axis indicates fraction methylation (0–1). (B) Methylation level over … see more
(A) h1 /nrpe1 cKO_49 has a deletion spanning exons 1–6. (B) h1 /nrpe1 cKO_63 has an indel induced frameshift leading to a premature stop codon in exon 1. (C) Both knockouts display complete loss of … see more
(A) Whole genome view of DNA methylation levels (average of 10 kb windows) in the genotypes indicated (as in Figure 3, including all five chromosomes). Y-axis indicates fraction methylation (0–1). (B… see more
(A) Number of hypo vs hyper DMRs in genotype comparisons indicated. (B) h1 hyper CHG DMR frequency density plot over Chromosomes 1–5. (C) Similarity matrix based on pairwise overlapping scores … see more
(A) Methylation metaplot in the genotypes indicated over regions of the genome that gain NRPE1 in h1. Arrow highlights the change in average CHH methylation in the h1/nrpe1 double mutant as compared … see more
Regions are highlighted in gray.
(A) Genome browser image showing SUVH1 re-localization in h1 mirrors that of NRPE1. (B–D) Boxplot inlaid violin plots (boxplot medians shown in blue) showing SUVH1 enrichment in wild-type (WT) vs h1 … see more
Differential expression analysis of RNA-seq data (from Choi et al., 2020 PMID:31732458) for genes involved in DNA methylation, comparing wild-type (WT) to h1 mutant genotypes.
Table showing bisulfite sequencing conversion efficiency estimation for the WGBS libraries generated and analysed in this paper.
List of .bed files used for analysis in this paper.