Zona incerta distributes a broad movement signal that modulates behavior
- Department of Neuroscience, University of Connecticut School of Medicine, United States
Figures
Figure 1
Zona incerta unit activity measured with electrophysiology in head-fixed mice on a spherical treadmill reveals two groups of neurons based on their activation during movement.
(A) Schematic of the head-fixed spherical treadmill setup. The frontal air tubes deliver air-puffs to the whiskers, and the posterior air tubes deliver air-puffs to the lower back. (B) Dark-field …
Figure 2
Response of zona incerta units to sensory stimuli applied to head-fixed mice on the spherical treadmill.
(A) PSTH of unit firing evoked by air-puffs to the whiskers (left panels) or lower back (right panels), and auditory stimuli consisting of a tone (8 kHz) or white noise (middle panels). Air-puffs …
Figure 3
Calcium imaging fiber photometry reveals that GABAergic zona incerta neurons activate during spontaneous exploratory movement.
(A) Parasagittal sections showing the optical fiber tract reaching zona incerta and GCaMP7f fluorescence expressed in GABAergic neurons around the fiber ending for two animals. The sections were …
Figure 4
GABAergic zona incerta neurons do not code the direction of spontaneous exploratory turning movements.
(A) F/Fo calcium imaging, overall movement, rotational movement, and angle of turning direction for detected movements classified by the turning direction (ipsiversive and contraversive; red and …
Figure 5
GABAergic zona incerta neurons discharge to auditory tones in association with movement.
(A) Example F/Fo calcium imaging and movement traces (mean ± standard error of the mean [SEM]) evoked from zona incerta neurons by auditory tones (1 s) of different saliency. The tones vary in …
Figure 6
GABAergic zona incerta activation in the context of signaled active avoidance.
(A) Arrangement of the shuttle box used during signaled avoidance tasks. (B) Behavioral performance during the four different avoidance procedures showing the percentage of active avoids (black …
Figure 7
GABAergic zona incerta neurons track the avoidance and escape movement.
(A) F/Fo and overall movement traces from CS onset (left) and response occurrence (right) for avoids during the AA4 procedure, which include three CSs that signal avoidance intervals of different …
Figure 8
Lesions of GABAergic zona incerta neurons disrupt movement but does not abolish signaled active avoidance learning or performance.
(A) Coronal Neurotrace (green) strained section of a Vgat-Cre mouse injected with a Cre-depedent AAV-dtA in the zona incerta to kill GABAergic neurons. The red fluorophore overlying and around zona …
Figure 9
Optogenetic inhibition of GABAergic zona incerta neurons during exploratory and goal-directed movements.
(A) Effects of unilateral trains (blue only) or continuous (blue or green) light on head bias (angle in degrees), and speed movement (cm/s) during exploration of an open field for No Opsin controls. …
Figure 10
Optogenetic excitation of GABAergic zona incerta neurons during exploratory and goal-directed movements.
Effects of unilateral (A) or bilateral (B) trains or continuous blue light on head bias (angle in degrees), and speed (cm/s) during exploration of an open field for Vgat-ZI-ChR2 mice. Unilateral …
Figure 11
Optogenetic excitation of GABAergic zona incerta pathways to PO, pedunculo-pontine tegmentum (PPT), and superior colliculus during exploratory movements.
Effects of unilateral (A) or bilateral (B) trains or continuous blue light on head bias (angle in degrees), and speed (cm/s) during exploration of an open field for Vgat-ZI-ChR2 → PO, Vgat-ZI-ChR2 → …
