(A) The PCR strategy used to detect Numb splice variants is shown. Red arrows depict the locations of the forward and reverse primers used. Exons (black boxes) are numbered 1–10. (B) cDNA was …
Data were analyzed by unpaired t-test. **p<0.01. Data as mean +/- STD. The lanes were cut from the same blot.
Original western blot analysis of data shown Figure 2 with anti-Numb (top image) and anti-GAPDH (bottom image).
Figure 2 original western blot analysis (anti-Numb, top, and anti-GAPDH, bottom) with the bands shown in Figure 2 highlighted and sample labels.
(A) Specific tension generated during tetanic contraction at the indicated frequencies is shown for weight normalized EDL muscle harvested from HSA-MCM/Numb(f/f) or HSA-MCM/Numb(f/f)/Numbl(f/f) mice …
Data were analyzed by one-way ANOVA with Tukey’s post hoc test. (A) F = 4.561, DFn = 3, DFd = 13; *p<0.05; (B) F = 3.679, DFn = 3, DFd = 13; (C) F = 0.9772, DFn = 3, DFd = 13. N = 3–6. Datas …
(A) Representative western blot of Numb immunoprecipitated from differentiated C2C12 myotubes. Input: original C2C12 lysate; Numb: goat anti-Numb IgG, control IgG: normal goat IgG. The blot was …
Original images of western blot analysis shown in Figure 5A (anti-Numb, top, and anti-GAPDH, bottom).
Original images of western blot analysis (anti-Numb, top, and anti-GAPDH, bottom) with the bands shown in Figure 5A highlighted and sample labels.
Original image of silver-stained IP gel shown in Figure 5B.
Original image of silver-stained IP gel with the bands shown in Figure 5B highlighted and sample labels.
(A) IgG: immunoprecipitation with control Ig; N: immunoprecipitation with anti-Numb; input: original C2C12 lysate. The upper panel was probed with anti-Septin 7, the lower panel with anti-Numb. (B–D)…
Original images of western blot analysis shown in Figure 6A (anti-Septin, left, and anti-Numb, right).
Figure 6A images of western blot analysis (anti-Septin 7, left, and anti-Numb, right) with the bands shown in Figure 6A highlighted and sample labels.
(A, B) Violin plots generated by Myoatlas showing abundance of Septin 7 (A) and Numb (B) mRNA in nuclei of TA muscle from 5-month-old mice (Dho et al., 1999). (C) Representative confocal microscopy …
Nuclei were labeled with DAPI (blue). (A–C) Myofiber from a control mouse (vehicle treated); (D–F) myofiber from a Numb cKO mouse (tamoxifen treated). Scale bar, 10 μm.
UniProt ID | Protein | Abundance ratio (Numb/control) | p-Value (control vs Numb) | Control count(out of 7) | Numb count (out of 7) |
---|---|---|---|---|---|
APCA4_MOUSE | Anaphase-promoting subunit 4 | ∞ | 0 | 0 | 7 |
NIPA_MOUSE | Nuclear-interacting partner of Alk | ∞ | 0 | 0 | 7 |
PDE4D_MOUSE | cAMP specific 3–5-cyclic phosphodiesterase 4D | ∞ | 0 | 0 | 6 |
WNK1_MOUSE | Serine-Threonine kinase Wnk1 | 289 | 5.4E–4 | 4 | 7 |
SEPT7_MOUSE | Septin 7 | 82.9 | 3.6E–3 | 4 | 7 |
APC 10_MOUSE | Anaphase-promoting complex subunit 10 | ∞ | 0 | 0 | 5 |
SEPT10_MOUSE | Septin 10 | ∞ | 0 | 0 | 4 |
HOME3_MOUSE | Homer protein homologue | ∞ | 0 | 0 | 4 |
TRAF7_MOUSE | E3 ubiquitin ligase Traf7 | ∞ | 0 | 0 | 4 |
RUVB1_MOUSE | RuvB-like 1 | ∞ | 0 | 0 | 5 |
SEPT2_MOUSE | Septin 2 | 379 | 0.01 | 5 | 7 |
SEPT9_MOUSE | Septin 9 | 148 | 0.01 | 4 | 7 |
MS/MS data for Septin 7 from representative IP sample (67) showing peptides abundance and example mass spectra.
MS/MS data for cAMP-specific 3,5 cyclic phosphodiesterase 4D (PDE4D) from representative IP sample (520) showing peptides abundance and example mass spectra.
MS/MS data for nuclear -interacting protein of ALK (NIPA) from representative IP sample (1617) showing peptides abundance and example mass spectra.
MS/MS data for anaphase-promoting complex subunit 2 (ANC2) from representative IP sample (67) showing peptides abundance and example mass spectra.
MS/MS data for anaphase-promoting complex subunit 4 (APC4) from representative IP sample (1617) showing peptides abundance and example mass spectra.
MS/MS data for anaphase-promoting complex subunit 10 (APC10) from representative IP sample (520) showing peptides abundance and example mass spectra.
List of MS/MS data for all the proteins for which peptides fragments were identified in the IP samples.
Manually curated annotation for the Numb-binding proteins identified by IP-MS/MS showing domains, function, and associated diseases including linkage to disorders of the skeleton.