Dysfunctional hippocampal-prefrontal network underlies a multidimensional neuropsychiatric phenotype following early-life seizure
- Department of Neuroscience and Behavioral Sciences, Ribeirão Preto Medical School, University of São Paulo, Brazil
- Department of Physiology and Biophysics Federal University of Minas Gerais, Brazil
- Laboratory of Molecular and Behavioral Neuroscience (LANEC), Federal University of Minas Gerais, Brazil
- Department of Psychiatry, University of Michigan Medical School, United States
- Department of Pathology, State University of Campinas, Brazil
- Neuroscience Discipline, Department of Neurology and Neurosurgery,Universidade Federal de São Paulo, Brazil
Figures
Figure 1
Early-life seizure (ELS) induction.
(A) Experimental design representing early-life Status epilepticus (SE) induction and monitoring. (B) Representative traces of local field potential (LFP) during baseline, motor seizure induced by …
Figure 2
Early-life seizure (ELS) induces cognitive and sensorimotor impairments.
(A) Experimental design representing early-life Status epilepticus (SE) induction, age of the animals during the experiments, and behavioral tests performed. (B) Weight gain after ELS induction. ELS …
Figure 3
Aberrant hippocampus-prefrontal cortex (HPC-PFC) synaptic plasticity in animals submitted to early-life seizure (ELS).
(A) Experimental design including high-frequency stimulation (HFS) to long-term potentiation (LTP) induction. (B) Electrode placement and representative electrolytic lesions in Cresyl Violet stained …
Figure 4
Early-life seizure (ELS) induces long-term neural inflammation, but not neuronal loss.
(A) 3-D brain diagram and coronal sections representing the brain regions investigated. (B–E) Representative photomicrographs from immunohistochemistry each pair of columns represents a group …
Figure 5
Cognitive impairment and sensorimotor deficits are distinctly correlated with abnormal long-term potentiation (LTP) and neuroinflammation.
(A) Steps performed in data processing and analysis for canonical correlation analysis (CCA). (B) CCA analysis showing control (CTRL) and early-life seizure (ELS) animals projected onto the first …
Figure 6
Classification of sleep-wake states and electrographic discharges in early-life seizure (ELS) rats.
(A) Electrode placement and representative electrolytic lesions in Nissl-stained coronal sections. (B) Representative spectrogram, electromyogram (EMG) root mean squared (RMS), and HPC theta/delta …
Figure 7
Increased HPC theta power during behavioral activity in early-life seizure (ELS) animals.
(A) Representative raw local field potentials (LFPs) (transparent black lines) and theta-filtered signal of control (CTRL) (black) and ELS (orange) from the PFC (left) and HPC (right) during active …
Figure 8
Early-life seizure (ELS) promotes abnormal gamma coordination in the PFC during behavioral activity.
ELS rats present an abnormal gamma activity in the PFC. (A) Grand-average amplitude correlogram for control (CTRL) (left) and ELS (right) group. Note the absence of amplitude correlation between …
Figure 9
ELS impairs hippocampus-prefrontal cortex (HPC-PFC) coherence during rapid eye movement (REM) sleep.
(A) Power spectrum densities (PSD) in the PFC during REM. (B) PSD in the HPC during REM. No significant alterations in theta power during REM are observed in early-life seizure (ELS) rats. (C) Theta …
Figure 10
Early-life seizure (ELS) rats display rapid eye movement (REM)-like oscillatory dynamics during active behavior.
(A) State map construction reflects the sleep-wake cycle and spectral dynamics for one representative control (CTRL) (top) and ELS (bottom) rat. PFC delta power is evident during non-REM (NREM), …
Figure 11
Early-life seizure (ELS) rats present behavioral and neurochemical sensitivity to dopaminergic activation.
(A) Experimental design representing quantification of locomotion in the open field, apomorphine (APO) injection, and neurochemical analysis. (B) Normalized locomotion across time blocks after APO …
Figure 12
HPC and PFC oscillatory dynamics discriminate early-life seizure (ELS) rats.
(A) A linear discriminant classifier (dotted orange line) using the first two principal components (PCs) of relevant electrophysiological variables completely distinguishes ELS from control (CTRL) …
