Homeostatic Control of Deep Sleep and Molecular Correlates of Sleep Pressure in Drosophila

Abstract
Data availability
Article and author information
Metrics

Abstract

Homeostatic control of sleep is typically addressed through mechanical stimulation-induced forced wakefulness and the measurement of subsequent increases in sleep. A major confound attends this approach: biological responses to deprivation may reflect a direct response to the mechanical insult rather than to the loss of sleep. Similar confounds accompany all forms of sleep deprivation and represent a major challenge to the field. Here we describe a new paradigm for sleep deprivation in Drosophila that fully accounts for sleep-independent effects. Our results reveal that deep sleep states are the primary target of homeostatic control and establish the presence of multi-cycle sleep rebound following deprivation. Furthermore, we establish that specific deprivation of deep sleep state results in state-specific homeostatic rebound. Finally, by accounting for the molecular effects of mechanical stimulation during deprivation experiments, we show that serotonin levels track sleep pressure in the fly's central brain. Our results illustrate the critical need to control for sleep-independent effects of deprivation when examining the molecular correlates of sleep pressure and call for a critical reassessment of work that has not accounted for such non-specific effects.

Data availability

All Raw Data is available at Dryad.

The following data sets were generated

1. Chowdhury B
2. Abhilash L
3. Ortega A
4. Liu S
5. Shafer OT
(2023) Homeostatic Control of Deep Sleep and Molecular Correlates of Sleep Pressure in Drosophila
Dryad Digital Repository, doi:10.5061/dryad.qnk98sfpp.

https://dx,doi.org/10.5061/dryad.qnk98sfpp

Article and author information

Author details

Budhaditya Chowdhury

Advanced Science Research Center, City University of New York, New York, United States

Competing interests
The authors declare that no competing interests exist.
Lakshman Abhilash

The Advanced Science Research Center, City University of New York, New York, United States

Competing interests
The authors declare that no competing interests exist.
Antonio Ortega

VIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium

Competing interests
The authors declare that no competing interests exist.
Sha Liu

VIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium

Competing interests
The authors declare that no competing interests exist.
Orie T Shafer

Advanced Science Research Center, City University of New York, New York, United States

For correspondence
oshafer@gc.cuny.edu

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0001-7177-743X

Funding

National Institute of Neurological Disorders and Stroke (R21NS131939)

Orie T Shafer

National Institute of Neurological Disorders and Stroke (R01NS077933)

Orie T Shafer

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.