(A–B) N-terminal fusion of Rad51-NTD/SCD, Rad53-SCD1, Hop1-SCD, Sml1-NTD, Sup35-PND, Ure2-UPD and New1-NPD promotes high-level expression of LacZ-NVH, respectively. The NVH tag contains an SV40 …
Raw and labelled images for blots shown in Figure 1.
The effects of WT and mutant Rad51-NTD on β-galactosidase activities (A), plasmid DNA copy numbers (B), relative steady-state levels of LacZ-NVH mRNA normalized to ACT1 (actin) mRNA (C), and …
The raw qPCR data of cDNA and gDNA in Figure 2.
(A) The steady-state protein levels of Rad51-NTD-LacZ-NVH and LacZ-NVH in WT and six protein homeostasis gene knockout mutants. (B–D) The impact of six protein homeostasis genes on the …
Raw and labelled images for blots shown in Figure 3.
(A) List of N-terminal tags with their respective length, numbers of S/T/Q/N amino acids, overall STQ or STQN percentages, and relative β-galactosidase activities. (B–D) Linear regressions between …
The amino acid sequences of WT and mutant IDRs are listed in Supplementary file 1e. Total protein lysates prepared from yeast cells expressing Rad51-NTD-LacZ-NVH (A), Sup35-PND-LacZ-NVH (B) or …
Raw and labelled images for blots shown in Figure 5.
The average usages of 20 different amino acids in 17 ciliate and 20 non-ciliate species.
The number of proteins containing different types of polyQ, polyQ/N and polyN tracts in 17 ciliate and 20 non-ciliate species.
The numbers and percentages of SCD and polyX proteins in 17 ciliate and 20 non-ciliate species.
The ratios of the overall number of X residues for each of the seven polyX motifs relative to those in the entire proteome of each species, respectively.
The codon usage frequency in 26 near-complete proteomes and 11 ciliate proteomes encoded by the transcripts generated as part of the Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP).
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment …
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
GO enrichment analyses revealing the SCD and polyX proteins involved in different biological processes in 6 ciliate and 20 non-ciliate species.
The percentages and numbers of SCD and polyX proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment and statistical analysis. The p values adjusted according to the Family-wise error rate (FWER) are shown. The overrepresented GO groups (adjusted p-values ≤0.001) are highlighted in red font.
The results of BLASTP searches using the 58 Tetrahymena thermophila proteins involved in xylan catabolysis.
The list of 124 Tetrahymena thermophila proteins involved in meiosis (kindly provided by Josef Loidl).
The numbers of SCD and polyX tracts in each protein are indicated.
The five ciliates with reassigned stops codon (TAAQ and TAGQ) are indicated in red. Stentor coeruleus, a ciliate with standard stop codons, is indicated in green.
The five ciliates with reassigned stops codon (TAAQ and TAGQ) are indicated in red. Stentor coeruleus, a ciliate with standard stop codons, is indicated in green.
The five ciliates with reassigned stops codon (TAAQ and TAGQ) are indicated in red. Stentor coeruleus, a ciliate with standard stop codons, is indicated in green.
The five ciliates with reassigned stops codon (TAAQ and TAGQ) are indicated in red. Stentor coeruleus, a ciliate with standard stop codons, is indicated in green.
The percentages and number of SCD-containing proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO enrichment …
The percentages and numbers of polyQ-containing proteins in our search that belong to each indicated Gene Ontology (GO) group are shown. GOfuncR (Huttenhower et al., 2009) was applied for GO …
Species | Source | ID | BUSCOProtein (%) | Protein# | TAA | TAG | TGA | CAA | CAG | TGC | TGT | TAC | TAT | TGG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NCBI genetic code: 1 | Non-ciliate eukaryotes | * | * | * | Q | Q | C | C | Y | Y | W | |||
Saccharomyces cerevisiae | UniProt | UP000002311 | 99.6 | 6062 | 0.16 | 0.08 | 0.01 | 2.77 | 0.89 | 0.63 | 1.03 | 0.86 | 3.10 | 0.93 |
Candida albicans | UniProt | UP000000559 | 98.8 | 6035 | 0.10 | 0.05 | 0.03 | 3.57 | 0.65 | 0.18 | 0.94 | 1.04 | 2.54 | 1.09 |
Candida auris | UniProt | UP000230249 | 97.4 | 5409 | 0.08 | 0.06 | 0.06 | 1.81 | 2.12 | 0.55 | 0.59 | 2.09 | 1.16 | 1.07 |
Candida tropicalis | UniProt | UP000002037 | 94.6 | 6226 | 0.10 | 0.07 | 0.03 | 3.61 | 0.66 | 0.14 | 0.96 | 0.95 | 2.62 | 0.98 |
Neurospora crassa | UniProt | UP000001805 | 99.2 | 10257 | 0.06 | 0.05 | 0.08 | 1.70 | 2.60 | 0.77 | 0.34 | 1.75 | 0.85 | 1.31 |
Magnaporthe oryzae | UniProt | UP000009058 | 98.6 | 12794 | 0.06 | 0.07 | 0.10 | 1.37 | 2.69 | 0.92 | 0.35 | 1.80 | 0.71 | 1.42 |
Trichoderma reesei | PMID: 34908505 | PRJNA382020 | 99.2 | 13735 | 0.06 | 0.06 | 0.11 | 1.17 | 2.95 | 0.95 | 0.32 | 1.80 | 0.83 | 1.42 |
Cryptococcus neoformans | UniProt | UP000002149 | 99.5 | 6743 | 0.07 | 0.06 | 0.05 | 2.06 | 1.79 | 0.48 | 0.55 | 1.39 | 1.14 | 1.37 |
Ustilago maydis | UniProt | UP000000561 | 99.4 | 6806 | 0.04 | 0.05 | 0.07 | 1.82 | 2.61 | 0.72 | 0.35 | 1.59 | 0.65 | 1.18 |
Taiwanofungus camphoratus | PMID: 35196809 | PRJNA615295 | 94.6 | 14019 | 0.05 | 0.06 | 0.11 | 1.57 | 2.19 | 0.70 | 0.57 | 1.38 | 1.22 | 1.36 |
Dictyostelium discoideum | UniProt | UP000002195 | 93.7 | 12734 | 0.16 | 0.01 | 0.01 | 4.86 | 0.19 | 0.15 | 1.27 | 0.52 | 3.02 | 0.73 |
Plasmodium falciparum | UniProt | UP000001450 | 99.1 | 5376 | 0.09 | 0.01 | 0.03 | 2.42 | 0.37 | 0.23 | 1.52 | 0.61 | 5.05 | 0.49 |
Drosophila melanogaster | UniProt | UP000000803 | 100 | 22088 | 0.08 | 0.07 | 0.05 | 1.56 | 3.61 | 1.32 | 0.54 | 1.84 | 1.08 | 0.99 |
Aedes aegypti | UniProt | UP000008820 | 99.4 | 18998 | 0.11 | 0.07 | 0.08 | 1.76 | 2.58 | 1.11 | 0.79 | 2.16 | 1.14 | 1.06 |
Caenorhabditis elegans | UniProt | UP000001940 | 100 | 26548 | 0.16 | 0.06 | 0.14 | 2.74 | 1.44 | 0.91 | 1.12 | 1.37 | 1.75 | 1.11 |
Danio rerio | UniProt | UP000000437 | 95.5 | 46844 | 0.11 | 0.06 | 0.14 | 1.18 | 3.35 | 1.12 | 1.13 | 1.70 | 1.26 | 1.16 |
Mus musculus | UniProt | UP000000589 | 99.7 | 55341 | 0.10 | 0.08 | 0.16 | 1.20 | 3.41 | 1.23 | 1.14 | 1.61 | 1.22 | 1.25 |
Homo sapiens | UniProt | UP000005640 | 99.5 | 79038 | 0.10 | 0.08 | 0.16 | 1.23 | 3.42 | 1.26 | 1.06 | 1.53 | 1.22 | 1.32 |
Arabidopsis thaliana | UniProt | UP000006548 | 100 | 39334 | 0.09 | 0.05 | 0.12 | 1.94 | 1.52 | 0.72 | 1.05 | 1.37 | 1.46 | 1.25 |
Chlamydomonas reinhardtii | UniProt | UP000006906 | 98.9 | 18829 | 0.03 | 0.04 | 0.06 | 0.59 | 4.05 | 1.1 | 0.22 | 1.45 | 0.24 | 1.16 |
NCBI genetic code: 6 | group I ciliates | → Q | → Q | * | Q | Q | C | C | Y | Y | W | |||
Tetrahymena thermophila | UniProt | UP000009168 | 98.9 | 26972 | 5.46 | 1.63 | 0.16 | 2.04 | 0.48 | 0.79 | 0.99 | 1.22 | 3.09 | 0.51 |
Paramecium tetraurelia | UniProt | UP000000600 | 98.8 | 39461 | 4.53 | 1.48 | 0.22 | 2.54 | 0.57 | 0.61 | 1.21 | 1.12 | 3.14 | 0.76 |
Oxytricha trifallax | UniProt | UP000006077 | 97.1 | 23559 | 3.63 | 1.57 | 0.15 | 2.68 | 1.07 | 0.59 | 0.56 | 1.44 | 2.27 | 0.58 |
Stylonychia lemnae | UniProt | UP000039865 | 97.1 | 20720 | 3.22 | 1.81 | 0.17 | 2.26 | 1.05 | 0.62 | 0.55 | 1.31 | 2.49 | 0.62 |
Pseudocohnilembus persalinus | UniProt | UP000054937 | 92.4 | 13175 | 7.36 | 1.39 | 0.18 | 1.76 | 0.37 | 0.32 | 1.00 | 1.00 | 3.26 | 0.61 |
NCBI genetic code: 6 | group II ciliates | → Q | → Q | * | Q | Q | C | C | Y | Y | W | |||
Aristerostoma | MMETSP | MMETSP0125 | 62.5 | 27868 | 0.96 | 1.04 | 0.15 | 2.65 | 0.97 | 0.71 | 0.68 | 1.35 | 2.49 | 0.8 |
Favella ehrenbergii | MMETSP | MMETSP0123 | 85.4 | 26477 | 0.72 | 1.51 | 0.16 | 1.88 | 3.06 | 1.11 | 0.25 | 2.06 | 0.71 | 0.83 |
Pseudokeronopsis | MMETSP | MMETSP0211 MMETSP1396 | 87.2 | 62574 | 1.04 | 1.37 | 0.16 | 2.05 | 2.58 | 0.94 | 0.44 | 2.18 | 1.40 | 0.78 |
Strombidium inclinatum | MMETSP | MMETSP0208 | 83.6 | 32210 | 0.64 | 1.28 | 0.11 | 1.63 | 3.50 | 0.83 | 0.24 | 2.12 | 0.69 | 0.7 |
Uronema spp. | MMETSP | MMETSP0018 | 52.6 | 13887 | 6.90 | 0.66 | 0.17 | 0.80 | 0.08 | 0.28 | 1.63 | 0.80 | 3.62 | 0.87 |
NCBI genetic code: 1 | group III ciliates | * | * | * | Q | Q | C | C | Y | Y | W | |||
Stentor coeruleus | UniProt | UP000187209 | 92.4 | 30969 | 0.16 | 0.08 | 0.01 | 2.77 | 0.89 | 0.63 | 1.03 | 0.86 | 3.1 | 0.93 |
Climacostomum virens | MMETSP | MMETSP1397 | 94.7 | 33899 | 0.11 | 0.09 | 0.04 | 1.79 | 2.20 | 1.38 | 0.60 | 2.60 | 0.85 | 1.06 |
Litonotus pictus | MMETSP | MMETSP0209 | 65.5 | 30222 | 0.08 | 0.03 | 0.01 | 2.12 | 1.52 | 0.63 | 0.77 | 1.83 | 2.25 | 0.54 |
Protocruzia adherens | MMETSP | MMETSP0216 | 74.9 | 40577 | 0.07 | 0.04 | 0.04 | 2.91 | 1.24 | 0.69 | 0.94 | 1.30 | 1.83 | 1.00 |
NCBI genetic code: 10 | group IV ciliate | * | * | → C | Q | Q | C | C | Y | Y | W | |||
Euplotes focardii | MMETSP | MMETSP0205 MMETSP0206 | 60.8 | 36659 | 0.23 | 0.06 | 0.51 | 2.43 | 1.23 | 0.49 | 0.84 | 1.28 | 2.38 | 0.87 |
NCBI genetic code: 4 | group IV ciliate | * | * | → W | Q | Q | C | C | Y | Y | W | |||
Blepharisma japonicum | MMETSP | MMETSP1395 | 81.9 | 22714 | 0.13 | 0.03 | 0.30 | 2.85 | 1.24 | 0.94 | 0.80 | 0.94 | 2.72 | 0.84 |
NCBI genetic code: 29 | group IV ciliate | → Y | → Y | * | Q | Q | C | C | Y | Y | W | |||
Mesodinium pulex | MMETSP | MMETSP0467 | 88.9 | 61058 | 0.29 | 0.56 | 0.13 | 0.77 | 3.33 | 1.53 | 0.25 | 1.78 | 0.34 | 1.29 |
Species | CAA | CAG | TAA | TAG |
---|---|---|---|---|
Saccharomyces cerevisiae S288c | 2.73 (62.6%Q) | 1.21 (37.4%Q) | 0.11 | 0.05 |
Candida albicans | 3.57 (84.6%Q) | 0.65 (15.4%Q) | 0.1 | 0.05 |
Candida auris | 1.81 (46.1%Q) | 2.12 (53.9%Q) | 0.08 | 0.06 |
Candida tropicalis | 3.61 (84.5%Q) | 0.66 (15.5%Q) | 0.1 | 0.07 |
Neurospora crassa | 1.70 (39.5%Q) | 2.60 (60.5%Q) | 0.06 | 0.05 |
Magnaporthe oryzae | 1.37 (33.7%Q) | 2.69 (66.3%Q) | 0.06 | 0.07 |
Trichoderma reesei | 1.17 (28.4%Q) | 2.95 (71.6%Q) | 0.06 | 0.06 |
Cryptococcus neoformans | 2.06 (53.5%Q) | 1.79 (46.5%Q) | 0.07 | 0.06 |
Ustilago maydis | 1.82 (41.3%Q) | 2.61 (58.7%Q) | 0.04 | 0.05 |
Taiwanofungus camphoratus | 1.57 (41.8%Q) | 2.19 (58.2%Q) | 0.05 | 0.06 |
Dictyostelium discoideum | 4.86 (96.2%Q) | 0.19 (3.8%Q) | 0.16 | 0.01 |
Plasmodium falciparum | 2.42 (86.7%Q) | 0.37 (13.3%Q) | 0.09 | 0.01 |
Drosophila melanogaster | 1.56 (13.4%Q) | 3.61 (86.6%Q) | 0.08 | 0.07 |
Aedes aegypti | 1.76 (40.6%Q) | 2.58 (59.4%Q) | 0.11 | 0.07 |
Caenorhabditis elegans | 2.74 (65.6%Q) | 1.44 (34.4%Q) | 0.16 | 0.06 |
Danio rerio | 1.18 (26.0%Q) | 3.35 (74.0%Q) | 0.11 | 0.06 |
Mus musculus | 1.20 (26.0%Q) | 3.41 (74.0%Q) | 0.1 | 0.08 |
Homo sapiens | 1.23 (26.5%Q) | 3.42 (73.5%Q) | 0.1 | 0.08 |
Arabidopsis thaliana | 1.94 (56.1%Q) | 1.52 (43.9%Q) | 0.09 | 0.05 |
Chlamydomonas reinhardtii | 0.59 (12.7%Q) | 4.05 (87.3%Q) | 0.03 | 0.04 |
Tetrahymena thermophila | 2.04 (21.2%Q) | 0.48 (5.0%Q) | 5.46 (56.8%Q) | 1.63 (17.0%Q) |
Paramecium tetraurelia | 2.54 (27.9%Q) | 0.57 (6.3%Q) | 4.53 (46.7%Q) | 1.48 (16.2%Q) |
Oxytricha trifallax | 2.68 (29.9%Q) | 1.07 (12.0%Q) | 3.63 (40.6%Q) | 1.57 (17.5%Q) |
Stylonychia lemnae | 2.26 (21.1%Q) | 1.05 (12.6%Q) | 3.22 (38.6%Q) | 1.81 (21.7%Q) |
Pseudocohnilembus persalinus | 1.76 (18.0%Q) | 0.37 (3.8%Q) | 7.36 (76.0%Q) | 1.39 (14.4%Q) |
Stentor coeruleus | 2.77 (75.7%Q) | 0.89 (24.3%Q) | 0.16 | 0.08 |
Species_name | CAA | CAG | TAA | TAG |
---|---|---|---|---|
Saccharomyces cerevisiae S288c | 2.73(62.6%Q) | 1.21(37.4%Q) | 0.11 | 0.05 |
Candida albicans | 3.57(84.6%Q) | 0.65(15.4%Q) | 0.1 | 0.05 |
Candida auris | 1.81(46.1%Q) | 2.12(53.9%Q) | 0.08 | 0.06 |
Candida tropicalis | 3.61(84.5%Q) | 0.66(15.5%Q) | 0.1 | 0.07 |
Neurospora crassa | 1.70(39.5%Q) | 2.60(60.5%Q) | 0.06 | 0.05 |
Magnaporthe oryzae | 1.37(33.7%Q) | 2.69(66.3%Q) | 0.06 | 0.07 |
Trichoderma reesei | 1.17(28.4%Q) | 2.95(71.6%Q) | 0.06 | 0.06 |
Cryptococcus neoformans | 2.06(53.5%Q) | 1.79(46.5%Q) | 0.07 | 0.06 |
Ustilago maydis | 1.82(41.3%Q) | 2.61(58.7%Q) | 0.04 | 0.05 |
Taiwanofungus camphoratus | 1.57(41.8%Q) | 2.19(58.2%Q) | 0.05 | 0.06 |
Dictyostelium discoideum | 4.86(96.2%Q) | 0.19(3.8%Q) | 0.16 | 0.01 |
Plasmodium falciparum | 2.42(86.7%Q) | 0.37(13.3%Q) | 0.09 | 0.01 |
Drosophila melanogaster | 1.56(13.4%Q) | 3.61(86.6%Q) | 0.08 | 0.07 |
Aedes aegypti | 1.76(40.6%Q) | 2.58(59.4%Q) | 0.11 | 0.07 |
Caenorhabditis elegans | 2.74(65.6%Q) | 1.44(34.4%Q) | 0.16 | 0.06 |
Danio rerio | 1.18(26.0%Q) | 3.35(74.0%Q) | 0.11 | 0.06 |
Mus musculus | 1.20(26.0%Q) | 3.41(74.0%Q) | 0.1 | 0.08 |
Homo sapiens | 1.23(26.5%Q) | 3.42(73.5%Q) | 0.1 | 0.08 |
Arabidopsis thaliana | 1.94(56.1%Q) | 1.52(43.9%Q) | 0.09 | 0.05 |
Chlamydomonas reinhardtii | 0.59(12.7%Q) | 4.05(87.3%Q) | 0.03 | 0.04 |
Tetrahymena thermophila | 2.04(21.2%Q) | 0.48(5.0%Q) | 5.46(56.8%Q) | 1.63(17.0%Q) |
Paramecium tetraurelia | 2.54(27.9%Q) | 0.57(6.3%Q) | 4.53(46.7%Q) | 1.48(16.2%Q) |
Oxytricha trifallax | 2.68(29.9%Q) | 1.07(12.0%Q) | 3.63(40.6%Q) | 1.57(17.5%Q) |
Stylonychia lemnae | 2.26(21.1%Q) | 1.05(12.6%Q) | 3.22(38.6%Q) | 1.81(21.7%Q) |
Pseudocohnilembus persalinus | 1.76(18.0%Q) | 0.37(3.8%Q) | 7.36(76.0%Q) | 1.39(14.4%Q) |
Stentor coeruleus | 2.77(75.7%Q) | 0.89(24.3%Q) | 0.16 | 0.08 |
Name | Genotype |
---|---|
WHY13008 | MATa, ho, leu2, ura3, his 4-X::LEU2-(NgoMIV;+ ori)-URA3, ERGI(SpeI), RAD51::hphMX4 |
WHY13283 | MATa, ho, leu2, ura3, his 4-X::LEU2-(NgoMIV;+ ori)-URA3, ERGI(SpeI), rad51A::hphMX4 |
WHY13416 | MATa, ho, leu2, ura3, his4-X::LEU2-(NgoMIV;+ori)-URA3, ERGI(SpeI), rad51 /_\N::hphMX4 |
WHY13744 | |
WHY13743 | |
WHY13741 | MATa, ho::LYS2, leu2, ura3, lys2, HIS4::LEU2-(BamHI;+ori), ERGI(SalI), SUP35 ^(PND ")-rad51AN::hphMX4" |
WHY10271 | MATa, ho::hisG, lys2, leu2::hisG, arg4-nsp, ura3 |
WHY13970 | MATa his 3Delta1, leu 2Delta0, met15 Delta0, ura3 Delta0 |
WHY13785 | MATa his 3Delta1, leu 2Delta0, met 15 Delta0, ura 3Delta0, hsp104::kanMX4 |
WHY14126 | MATa his 3Delta1, leu 2Delta0, met 15 Delta0, ura 3Delta0, new 1::kan MX4 |
WHY14129 | MATa his 3Delta1, leu 2Delta0, met 15 Delta0, ura 3Delta0, doa 4::kan MX4 |
WHY14227 | |
WHY13989 | MATa his 3Delta1, leu 2Delta0, met 15 Delta0, ura 3Delta0,san 1:'kan MX4 |
WHY14132 | MATa his 3Delta1, leu 2Delta0, met 15 Delta0, ura 3Delta0, oaz 1:'kan MX4 |
Plasids, yeast strains, PCR primers, and home-made software tools.