(A) The maxilla of the larva (left), the representative spike traces of the responses of lateral sensilla styloconica on larval maxillary galea to eight sugars at 10 mM (middle), and quantifications …
Electrophysiological responses of larval and adult contact chemosensilla in Helicoverpa armigera to sugars.
(Figure 1A) The electrophysiological responses of the lateral sensilla styloconica on the maxillary galea of H. armigera 5th instar larvae to test compounds. (Figure 1B) The electrophysiological responses of the lateral sensilla styloconica on the maxillary galea of H. armigera 5th instar larvae to sucrose and fucose at different concentrations. (Figure 1C) The electrophysiological responses of the contact chemosensilla on antennae of H. armigera female adults to test compounds. (Figure 1D) The electrophysiological responses of the contact chemosensilla on the antennae of H. armigera female adults to sucrose, fucose and fructose at different concentrations. (Figure 1E) The electrophysiological responses of the contact chemosensilla on the tarsi of H. armigera female adults to test compounds. (Figure 1F) The electrophysiological responses of the contact chmosensilla on the tarsi of H. armigera female adults to sucrose, fucose, and fructose at different concentrations. (Figure 1G) The electrophysiological responses of the contact chemosensilla on the proboscis of H. armigera female adults to test compounds. (Figure 1H) The electrophysiological responses of the contact chemosensilla on the proboscis of H. armigera female adults to sucrose, fucose, and fructose at different concentrations.
(A) The representative spike traces and waveforms of lateral sensilla styloconica on larval maxillary galea and contact chemosensilla on female antennae, tarsi and proboscis responding to test …
Electrophysiological responses of contact chemosensilla in Helicoverpa armigera to sugars.
(Figure 1—figure supplement 1C) The electrophysiological responses of the medial sensilla styloconica on maxillary galea of H. armigera 5th instar larvae to test compounds. (Figure 1—figure supplement 1D) The electrophysiological responses of the lateral sensilla styloconica on maxillary galea of H. armigera 5th instar larvae to fucose, sucrose and their mixture.
(A) Feeding responses and the preference index (PI) value of 5th instar larvae to eight sugars painted on the cabbage leaf discs at 10 mM in two-choice tests. ** p<0.01; ns indicates no …
Behavioral responses of Helicoverpa armigera larvae and adults to sugars.
(Figure 2A) Feeding area of H. armigera 5th instar larvae in two-choice tests of eight sugars. (Figure 2B) Feeding area of H. armigera 5th instar larvae in two-choice tests of sucrose at different concentrations. (Figure 2C) Proboscis extension reflex (PER) in adult females upon antennal stimulation by eight sugars. (Figure 2D) PER in adult females upon tarsal stimulation by eight sugars. (Figure 2E) PER in adult females upon antennal stimulation by different concentrations of sucrose, fucose, and fructose. (Figure 2F) PER in adult females upon tarsal stimulation by different concentrations of sucrose, fucose, and fructose.
Data are mean ± SEM; ** p<0.01; ns indicates no significance (paired t test, n=20).
Feeding area of H. armigera 5th-instar larvae in two-choice tests to fructose at different concentrations.
(A) The phylogenetic tree of insect sugar GRs. Diptera (orange): Aaeg, Aedes aegypti; Agam, Anopheles gambiae; Dmel, Drosophila melanogaster. Hymenoptera (blue): Am, Apis mellifera. Lepidoptera …
The expression levels of sugar GRs in larval maxilla and female adult antennae, tarsi and proboscis of Helicoverpa armigera.
(Figure 3B) Expression levels of sugar GRs in the maxillary galea of H. armigera larvae. (Figure 3C) Expression levels of sugar GRs in the antennae of H. armigera female adults. (Figure 3D) Expression levels of sugar GRs in the tarsi of H. armigera female adults. (Figure 3E) Expression levels of sugar GRs in the proboscis of H. armigera female adults.
(A) Relative expression levels of sugar GRs in male antennae determined by qRT-PCR. (B) Relative expression levels of sugar GRs in male tarsi. (C) Relative expression levels of sugar GRs in male …
Expression patterns of sugar GRs in taste organs of Helicoverpa armigera male adults.
(Figure 3—figure supplement 1A) Expression levels of sugar GRs in the antennae of H. armigera male adults. (Figure 3—figure supplement 1B) Expression levels of sugar GRs in the tarsi of H. armigera male adults. (Figure 3—figure supplement 1C) Expression levels of sugar GRs in the proboscis of H. armigera male adults.
(A) The representative traces of the oocytes expressing Gr10, Gr6, and Gr10 +Gr6 to 11 sugars at 100 mM. (B) The responses of the oocytes expressing Gr10, Gr6, and Gr10 +Gr6 to sugars at 100 mM …
The inward current responses of the Xenopus oocytes expressing sugar GRs of Helicoverpa armigera to sugars.
(A) Heat-map signal indicates the mean of the responses to eleven sugars at 100 mM of the oocytes expressing H. armigera sugar GRs (H2O: mannose, n=5; other sugars and ringer solution: n=6. Gr4: …
The inward current responses of Xenopus oocytes expressing sugar GRs of Helicoverpa armigera to sugars.
(Figure 4—figure supplement 1A) The inward current responses of the oocytes expressing sugar GRs to 100 mM sugars. (Figure 4—figure supplement 1C) The inward current responses of the oocytes expressing Gr5 +Gr6 to 100 mM sugars.
(A) The cross process of obtaining Gr10-/-. (B) The genomic structure of Gr10, the single-guide RNA (sgRNA) targeting sequence (in green), and representative chromatograms of direct sequencing of …
(A) The alignment of the nucleic acid sequences based on Gr10 transcripts in WT and Gr10-/-. (B) The alignment of the nucleic acid sequences based on Gr6 transcripts in WT and Gr6-/-. The green …
(A) Representative chromatograms of potential off-target PCR products obtained from the wild type (WT) and Gr6-/-. (B) The representative spike traces (the left) and quantifications of the firing …
The source data of potential off-target effects detection.
(Figure 5—figure supplement 2B) The electrophysiological responses of the medial sensilla styloconica of larvae in the wild type (WT) and two mutants of H. armigera to KCl and xylose. (Figure 5—figure supplement 2C) The larval body weight, adult lifespan, and the number of eggs laid by females of the WT and two mutants of H. armigera.
(A) The representative spike traces of lateral sensilla styloconica on larval maxillary galea. (B) Quantifications of the firing rates of the lateral sensilla styloconica on larval maxillary galea …
Electrophysiological responses of larval and adult contact chemosensilla in WT, Gr10-/- and Gr6-/- of Helicoverpa armigera to sucrose and other compounds.
(Figure 6B) The electrophysiological responses of the lateral sensilla styloconica on the maxillary galea of larvae in the WT and two mutants of H. armigera to test compounds. (Figure 6D) The electrophysiological responses of the contact chemosensilla on the antennae of female adults in the WT and two mutants of H. armigera to test compounds. (Figure 6F) The electrophysiological responses of the contact chemosensilla on the fore leg tarsi of female adults in the WT and two mutants of H. armigera to test compounds. (Figure 6H) The electrophysiological responses of the contact chemosensilla on the proboscis of female adults in the WT and two mutants of H. armigera to test compounds.
(A) Feeding area of 5th instar larvae in two-choice tests and the PI value to 10 mM sucrose (n=20). ** p<0.01; ns indicates no significance, p ≥ 0.05 (paired t test). Suc, sucrose. (B) Feeding area …
Behavioral responses of WT, Gr10-/- and Gr6-/- larvae and adults of Helicoverpa armigera to sugars and plant tissues.
(Figure 7A) Feeding area of the WT, Gr10-/- and Gr6-/- 5th instar larvae of H. armigera in two-choice tests of 10 mM sucrose. (Figure 7B) Feeding area of the WT, Gr10-/- and Gr6-/- 5th instar larvae of H. armigera in two-choice tests of 100 mM sucrose. (Figure 7C) The feeding amount of the 5th instar larvae in no-choice tests of cabbage leaf, corn seed, pea seed, pepper fruit, and tomato fruit. (Figure 7D) Proboscis extension reflex (PER) in females of the WT and Gr10-/- and Gr6-/- mutants of H. armigera upon antennal stimulation by sucrose concentrations. (Figure 7E) PER in females of the WT and Gr10-/- and Gr6-/- mutants of H. armigera upon antennal stimulation by fucose concentrations. (Figure 7F) PER in females of the WT and Gr10-/- and Gr6-/- mutants of H. armigera upon antennal stimulation by fructose concentrations. (Figure 7G) PER in females of the WT and Gr10-/- and Gr6-/- mutants of H. armigera upon tarsal stimulation by sucrose concentrations. (Figure 7H) PER in females of the WT and Gr10-/- and Gr6-/- mutants of H. armigera upon tarsal stimulation by fucose concentrations. (Figure 7I) PER in females of the WT and Gr10-/- and Gr6-/- mutants of H. armigera upon tarsal stimulation by fructose concentrations.
(A) Feeding area and the PI value to 10 mM fructose. (B) Feeding area and the PI value to 100 mM fructose. Data are mean ± SEM, n=20, paired t test, ** p<0.01. Fru, fructose. Supplementary file 1. …
Behavioral responses of WT, Gr10-/- and Gr6-/- larvae and adults of Helicoverpa armigera to fructose.
(Figure 7—figure supplement 1A) Feeding area of the WT, Gr10-/- and Gr6-/- 5th instar larvae of H. armigera in two-choice tests of 10 mM fructose. (Figure 7—figure supplement 1B) Feeding area of the WT, Gr10-/- and Gr6-/- 5th instar larvae of H. armigera in two-choice tests of 100 mM fructose.
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Gene (Helicoverpa armigera) | Gr4 | GenBank | OP251144 | More details about this gene see Supplementary file 1. |
Gene (H. armigera) | Gr5 | GenBank | OP251145 | More details about this gene see Supplementary file 1 |
Gene (H. armigera) | Gr6 | GenBank | OP251146 | More details about this gene see Supplementary file 1 |
Gene (H. armigera) | Gr7 | GenBank | OP251147 | More details about this gene see Supplementary file 1 |
Gene (H. armigera) | Gr8 | GenBank | OP251148 | More details about this gene see Supplementary file 1 |
Gene (H. armigera) | Gr9 | GenBank | XM_049843199 | More details about this gene see Supplementary file 1 |
Gene (H. armigera) | Gr10 | GenBank | OP251149 | More details about this gene see Supplementary file 1 |
Gene (H. armigera) | Gr11 | GenBank | OP251150 | More details about this gene see Supplementary file 1 |
Gene (H. armigera) | Gr12 | GenBank | OP251151 | More details about this gene see Supplementary file 1 |
Commercial assay or kit | RNeasy Plus Universal Mini Kit | Qiagen | Cat# 73404 | |
Commercial assay or kit | Q5 High-Fidelity DNA Polymerase | NEB | Cat# M0491 | |
Commercial assay or kit | TransStart FastPfu DNA Polymerase | TransGen Biotech | Cat# AP221-01 | |
Commercial assay or kit | M-MLV reverse transcriptase | Promega | Cat# M1701 | |
Commercial assay or kit | SYBR Premix Ex Taq | Takara | Cat# RR820 | |
Commercial assay or kit | mMESSAGE mMACHINE SP6 | Ambion | Cat# AM1340 | |
Commercial assay or kit | GeneArt gRNA Clean Up Kit | Invitrogen | Cat#A29377 | |
Commercial assay or kit | GeneArt gRNA Prep Kit | Invitrogen | Cat#A29377 | |
Commercial assay or kit | TrueCut Cas9 protein 2 | Invitrogen | Cat#A36498 | |
Commercial assay or kit | Animal Tissue PCR Kit | TransGen Biotech | Cat#AD201-01 | |
Chemical compound, drug | L - (+) - Arabinose | Sigma-Aldrich | CAS: 5328-37-0 | |
Chemical compound, drug | D - (-) - Fructose | Sigma-Aldrich | CAS: 57-48-7 | |
Chemical compound, drug | L - (-) - Fucose | Sigma-Aldrich | CAS: 2438-80-4 | |
Chemical compound, drug | D - (+) - Galactose | Sigma-Aldrich | CAS: 59-23-4 | |
Chemical compound, drug | D - (+) - Glucose | Sigma-Aldrich | CAS: 50-99-7 | |
Chemical compound, drug | D - (+) - Mannose | Sigma-Aldrich | CAS: 3458-28-4 | |
Chemical compound, drug | D - (+) - Xylose | Sigma-Aldrich | CAS: 58-86-6 | |
Chemical compound, drug | D - Lactose monohydrate | Sigma-Aldrich | CAS: 64044-51-1 | |
Chemical compound, drug | D - (+)-Maltose monohydrate | Sigma-Aldrich | CAS: 6363-53-7 | |
Chemical compound, drug | Sucrose | Sigma-Aldrich | CAS: 57-50-1 | |
Chemical compound, drug | D - (+) - Trehalose dihydrate | Sigma-Aldrich | CAS: 6138-23-4 | |
Chemical compound, drug | Sodium chloride | Sigma-Aldrich | CAS: 7647-14-5 | |
Chemical compound, drug | Potassium chloride | Sigma-Aldrich | CAS: 7447-40-7 | |
Chemical compound, drug | Magnesium chloride hexahydrate | Sigma-Aldrich | CAS: 7791-18-6 | |
Chemical compound, drug | HEPES | Sigma-Aldrich | CAS: 7365-45-9 | |
Software, algorithm | SAPID Tools software version 3.5 | Smith et al., 1990; | ||
Software, algorithm | Autospike 3.7 | Syntech | ||
Software, algorithm | MAFFT version 7.455 | Rozewicki et al., 2019 | ||
Software, algorithm | trimAI version 1.4 | Capella-Gutiérrez et al., 2009; | ||
Software, algorithm | IQ-tree version 6.8 | Nguyen et al., 2015 | http://iqtree.org/ | |
Software, algorithm | pCLAMP software version 10.4.2.0 | Axon Instruments Inc | RRID:SCR_011323 | |
Software, algorithm | SnapGene software version 4.3.8 | Insightful Science | https://www.snapgene.com/ | |
Software, algorithm | SeqMan software version 7.1 | DNASTAR | https://www.dnastar.com/ | |
Software, algorithm | SPSS 20 | IBM | https://www.ibm.com | |
Software, algorithm | GraphPad Prism 8.2.1 | Dotmatics | https://www.graphpad.com/ | |
Other | Primers for full-length cloning of GRs | This paper | see Supplementary file 2 | |
Other | Primers for qRT-PCR | This paper | see Supplementary file 3 | |
Other | Primers for Xenopus oocytes expression system | This paper | see Supplementary file 4 | |
Other | Primers for experiments of mutant strains establishment | This paper | see Supplementary file 5 |
GenBank accession numbers for sugar gustatory receptors used in this study.
The primer sequences used in PCR for full-length cloning of GRs.
F: forward strand; R: reverse strand.
The primer sequences used in qRT-PCR for GR gene expression quantification.
F: forward strand; R: reverse strand.
The primer sequences used in cDNA synthesis for Xenopus oocytes expression system.
The italic sequences are protective bases, underline sequences are restriction enzymes, bold sequences are Kozak sequences. F: forward strand; R: reverse strand.
The primer sequences used in the experiments of Gr10 and Gr6 mutants establishment.
F: forward strand; R: reverse strand.
Summary of the CRISPR/Cas9 directed mutation rates from G0 to G2 in the establishment of Gr10 and Gr6 mutants.