The larva and adult of Helicoverpa armigera use differential gustatory receptors to sense sucrose

  1. Shuai-Shuai Zhang
  2. Pei-Chao Wang
  3. Chao Ning
  4. Ke Yang
  5. Guo-Cheng Li
  6. Lin-Lin Cao
  7. Ling-Qiao Huang
  8. Chen-Zhu Wang  Is a corresponding author
  1. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, China
  2. Chinese Academy of Sciences Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, China
8 figures, 1 table and 7 additional files

Figures

Figure 1 with 1 supplement
Electrophysiological responses of larval and adult contact chemosensilla in Helicoverpa armigera to sugars.

(A) The maxilla of the larva (left), the representative spike traces of the responses of lateral sensilla styloconica on larval maxillary galea to eight sugars at 10 mM (middle), and quantifications …

Figure 1—source data 1

Electrophysiological responses of larval and adult contact chemosensilla in Helicoverpa armigera to sugars.

(Figure 1A) The electrophysiological responses of the lateral sensilla styloconica on the maxillary galea of H. armigera 5th instar larvae to test compounds. (Figure 1B) The electrophysiological responses of the lateral sensilla styloconica on the maxillary galea of H. armigera 5th instar larvae to sucrose and fucose at different concentrations. (Figure 1C) The electrophysiological responses of the contact chemosensilla on antennae of H. armigera female adults to test compounds. (Figure 1D) The electrophysiological responses of the contact chemosensilla on the antennae of H. armigera female adults to sucrose, fucose and fructose at different concentrations. (Figure 1E) The electrophysiological responses of the contact chemosensilla on the tarsi of H. armigera female adults to test compounds. (Figure 1F) The electrophysiological responses of the contact chmosensilla on the tarsi of H. armigera female adults to sucrose, fucose, and fructose at different concentrations. (Figure 1G) The electrophysiological responses of the contact chemosensilla on the proboscis of H. armigera female adults to test compounds. (Figure 1H) The electrophysiological responses of the contact chemosensilla on the proboscis of H. armigera female adults to sucrose, fucose, and fructose at different concentrations.

https://cdn.elifesciences.org/articles/91711/elife-91711-fig1-data1-v1.xlsx
Figure 1—figure supplement 1
Electrophysiological responses of contact chemosensilla in Helicoverpa armigera to sugars.

(A) The representative spike traces and waveforms of lateral sensilla styloconica on larval maxillary galea and contact chemosensilla on female antennae, tarsi and proboscis responding to test …

Figure 1—figure supplement 1—source data 1

Electrophysiological responses of contact chemosensilla in Helicoverpa armigera to sugars.

(Figure 1—figure supplement 1C) The electrophysiological responses of the medial sensilla styloconica on maxillary galea of H. armigera 5th instar larvae to test compounds. (Figure 1—figure supplement 1D) The electrophysiological responses of the lateral sensilla styloconica on maxillary galea of H. armigera 5th instar larvae to fucose, sucrose and their mixture.

https://cdn.elifesciences.org/articles/91711/elife-91711-fig1-figsupp1-data1-v1.xlsx
Figure 2 with 1 supplement
Behavioral responses of Helicoverpa armigera larvae and adults to sugars.

(A) Feeding responses and the preference index (PI) value of 5th instar larvae to eight sugars painted on the cabbage leaf discs at 10 mM in two-choice tests. ** p<0.01; ns indicates no …

Figure 2—source data 1

Behavioral responses of Helicoverpa armigera larvae and adults to sugars.

(Figure 2A) Feeding area of H. armigera 5th instar larvae in two-choice tests of eight sugars. (Figure 2B) Feeding area of H. armigera 5th instar larvae in two-choice tests of sucrose at different concentrations. (Figure 2C) Proboscis extension reflex (PER) in adult females upon antennal stimulation by eight sugars. (Figure 2D) PER in adult females upon tarsal stimulation by eight sugars. (Figure 2E) PER in adult females upon antennal stimulation by different concentrations of sucrose, fucose, and fructose. (Figure 2F) PER in adult females upon tarsal stimulation by different concentrations of sucrose, fucose, and fructose.

https://cdn.elifesciences.org/articles/91711/elife-91711-fig2-data1-v1.xlsx
Figure 2—figure supplement 1
Feeding responses and the PI value of 5th instar larvae of Helicoverpa armigera to different concentrations of fructose painted on the cabbage leaf discs in two-choice tests.

Data are mean ± SEM; ** p<0.01; ns indicates no significance (paired t test, n=20).

Figure 2—figure supplement 1—source data 1

Feeding area of H. armigera 5th-instar larvae in two-choice tests to fructose at different concentrations.

https://cdn.elifesciences.org/articles/91711/elife-91711-fig2-figsupp1-data1-v1.xlsx
Figure 3 with 1 supplement
The phylogenetic relationship and the expression level of sugar GRs in larval maxilla and female adult antennae, tarsi and proboscis of Helicoverpa armigera.

(A) The phylogenetic tree of insect sugar GRs. Diptera (orange): Aaeg, Aedes aegypti; Agam, Anopheles gambiae; Dmel, Drosophila melanogaster. Hymenoptera (blue): Am, Apis mellifera. Lepidoptera …

Figure 3—source data 1

The expression levels of sugar GRs in larval maxilla and female adult antennae, tarsi and proboscis of Helicoverpa armigera.

(Figure 3B) Expression levels of sugar GRs in the maxillary galea of H. armigera larvae. (Figure 3C) Expression levels of sugar GRs in the antennae of H. armigera female adults. (Figure 3D) Expression levels of sugar GRs in the tarsi of H. armigera female adults. (Figure 3E) Expression levels of sugar GRs in the proboscis of H. armigera female adults.

https://cdn.elifesciences.org/articles/91711/elife-91711-fig3-data1-v1.xlsx
Figure 3—figure supplement 1
Expression patterns of sugar GRs in taste organs of Helicoverpa armigera male adults.

(A) Relative expression levels of sugar GRs in male antennae determined by qRT-PCR. (B) Relative expression levels of sugar GRs in male tarsi. (C) Relative expression levels of sugar GRs in male …

Figure 3—figure supplement 1—source data 1

Expression patterns of sugar GRs in taste organs of Helicoverpa armigera male adults.

(Figure 3—figure supplement 1A) Expression levels of sugar GRs in the antennae of H. armigera male adults. (Figure 3—figure supplement 1B) Expression levels of sugar GRs in the tarsi of H. armigera male adults. (Figure 3—figure supplement 1C) Expression levels of sugar GRs in the proboscis of H. armigera male adults.

https://cdn.elifesciences.org/articles/91711/elife-91711-fig3-figsupp1-data1-v1.xlsx
Figure 4 with 1 supplement
The inward current responses of the Xenopus oocytes expressing sugar GRs of Helicoverpa armigera to sugars.

(A) The representative traces of the oocytes expressing Gr10, Gr6, and Gr10 +Gr6 to 11 sugars at 100 mM. (B) The responses of the oocytes expressing Gr10, Gr6, and Gr10 +Gr6 to sugars at 100 mM …

Figure 4—source data 1

The inward current responses of the Xenopus oocytes expressing sugar GRs of Helicoverpa armigera to sugars.

(Figure 4B) The inward current responses of the oocyte expressing Gr10, Gr6 and Gr10 +Gr6 to 100 mM sugars. (Figure 4D) The inward current responses of the oocyte expressing Gr10, Gr6 and Gr10 +Gr6 to sucrose, fucose, and fructose at different concentrations.

https://cdn.elifesciences.org/articles/91711/elife-91711-fig4-data1-v1.xlsx
Figure 4—figure supplement 1
The inward current responses and representative traces of Xenopus oocytes expressing sugar GRs of Helicoverpa armigera.

(A) Heat-map signal indicates the mean of the responses to eleven sugars at 100 mM of the oocytes expressing H. armigera sugar GRs (H2O: mannose, n=5; other sugars and ringer solution: n=6. Gr4: …

Figure 4—figure supplement 1—source data 1

The inward current responses of Xenopus oocytes expressing sugar GRs of Helicoverpa armigera to sugars.

(Figure 4—figure supplement 1A) The inward current responses of the oocytes expressing sugar GRs to 100 mM sugars. (Figure 4—figure supplement 1C) The inward current responses of the oocytes expressing Gr5 +Gr6 to 100 mM sugars.

https://cdn.elifesciences.org/articles/91711/elife-91711-fig4-figsupp1-data1-v1.xlsx
Figure 5 with 2 supplements
Establishment of Gr10 and Gr6 homozygous mutants (Gr10-/- and Gr6-/-) of Helicoverpa armigera via CRISPR/Cas9.

(A) The cross process of obtaining Gr10-/-. (B) The genomic structure of Gr10, the single-guide RNA (sgRNA) targeting sequence (in green), and representative chromatograms of direct sequencing of …

Figure 5—figure supplement 1
Confirmation of the deletion of Gr10 and Gr6 in Gr10-/- and Gr6-/- mutants at mRNA level.

(A) The alignment of the nucleic acid sequences based on Gr10 transcripts in WT and Gr10-/-. (B) The alignment of the nucleic acid sequences based on Gr6 transcripts in WT and Gr6-/-. The green …

Figure 5—figure supplement 2
The potential off-target effects detection.

(A) Representative chromatograms of potential off-target PCR products obtained from the wild type (WT) and Gr6-/-. (B) The representative spike traces (the left) and quantifications of the firing …

Figure 5—figure supplement 2—source data 1

The source data of potential off-target effects detection.

(Figure 5—figure supplement 2B) The electrophysiological responses of the medial sensilla styloconica of larvae in the wild type (WT) and two mutants of H. armigera to KCl and xylose. (Figure 5—figure supplement 2C) The larval body weight, adult lifespan, and the number of eggs laid by females of the WT and two mutants of H. armigera.

https://cdn.elifesciences.org/articles/91711/elife-91711-fig5-figsupp2-data1-v1.xlsx
Electrophysiological responses of larval and adult contact chemosensilla in WT, Gr10-/- and Gr6-/- of Helicoverpa armigera to sucrose and other compounds.

(A) The representative spike traces of lateral sensilla styloconica on larval maxillary galea. (B) Quantifications of the firing rates of the lateral sensilla styloconica on larval maxillary galea …

Figure 6—source data 1

Electrophysiological responses of larval and adult contact chemosensilla in WT, Gr10-/- and Gr6-/- of Helicoverpa armigera to sucrose and other compounds.

(Figure 6B) The electrophysiological responses of the lateral sensilla styloconica on the maxillary galea of larvae in the WT and two mutants of H. armigera to test compounds. (Figure 6D) The electrophysiological responses of the contact chemosensilla on the antennae of female adults in the WT and two mutants of H. armigera to test compounds. (Figure 6F) The electrophysiological responses of the contact chemosensilla on the fore leg tarsi of female adults in the WT and two mutants of H. armigera to test compounds. (Figure 6H) The electrophysiological responses of the contact chemosensilla on the proboscis of female adults in the WT and two mutants of H. armigera to test compounds.

https://cdn.elifesciences.org/articles/91711/elife-91711-fig6-data1-v1.xlsx
Figure 7 with 1 supplement
Behavioral responses of WT, Gr10-/- and Gr6-/- larvae and adults of Helicoverpa armigera to sugars and plant tissues.

(A) Feeding area of 5th instar larvae in two-choice tests and the PI value to 10 mM sucrose (n=20). ** p<0.01; ns indicates no significance, p ≥ 0.05 (paired t test). Suc, sucrose. (B) Feeding area …

Figure 7—source data 1

Behavioral responses of WT, Gr10-/- and Gr6-/- larvae and adults of Helicoverpa armigera to sugars and plant tissues.

(Figure 7A) Feeding area of the WT, Gr10-/- and Gr6-/- 5th instar larvae of H. armigera in two-choice tests of 10 mM sucrose. (Figure 7B) Feeding area of the WT, Gr10-/- and Gr6-/- 5th instar larvae of H. armigera in two-choice tests of 100 mM sucrose. (Figure 7C) The feeding amount of the 5th instar larvae in no-choice tests of cabbage leaf, corn seed, pea seed, pepper fruit, and tomato fruit. (Figure 7D) Proboscis extension reflex (PER) in females of the WT and Gr10-/- and Gr6-/- mutants of H. armigera upon antennal stimulation by sucrose concentrations. (Figure 7E) PER in females of the WT and Gr10-/- and Gr6-/- mutants of H. armigera upon antennal stimulation by fucose concentrations. (Figure 7F) PER in females of the WT and Gr10-/- and Gr6-/- mutants of H. armigera upon antennal stimulation by fructose concentrations. (Figure 7G) PER in females of the WT and Gr10-/- and Gr6-/- mutants of H. armigera upon tarsal stimulation by sucrose concentrations. (Figure 7H) PER in females of the WT and Gr10-/- and Gr6-/- mutants of H. armigera upon tarsal stimulation by fucose concentrations. (Figure 7I) PER in females of the WT and Gr10-/- and Gr6-/- mutants of H. armigera upon tarsal stimulation by fructose concentrations.

https://cdn.elifesciences.org/articles/91711/elife-91711-fig7-data1-v1.xlsx
Figure 7—figure supplement 1
Feeding responses and the PI value of 5th instar larvae of WT, Gr10-/- and Gr6-/- of Helicoverpa armigera to fructose painted on the cabbage leaf discs in two-choice tests.

(A) Feeding area and the PI value to 10 mM fructose. (B) Feeding area and the PI value to 100 mM fructose. Data are mean ± SEM, n=20, paired t test, ** p<0.01. Fru, fructose. Supplementary file 1. …

Figure 7—figure supplement 1—source data 1

Behavioral responses of WT, Gr10-/- and Gr6-/- larvae and adults of Helicoverpa armigera to fructose.

(Figure 7—figure supplement 1A) Feeding area of the WT, Gr10-/- and Gr6-/- 5th instar larvae of H. armigera in two-choice tests of 10 mM fructose. (Figure 7—figure supplement 1B) Feeding area of the WT, Gr10-/- and Gr6-/- 5th instar larvae of H. armigera in two-choice tests of 100 mM fructose.

https://cdn.elifesciences.org/articles/91711/elife-91711-fig7-figsupp1-data1-v1.xlsx
Two gustatory receptors in the cotton bollworm, Helicoverpa armigera mainly mediate taste sensation of sugars in the larval and adult foods.

Tables

Appendix 1—key resources table
Reagent type (species) or resourceDesignationSource or referenceIdentifiersAdditional information
Gene (Helicoverpa armigera)Gr4GenBankOP251144More details about this gene see Supplementary file 1.
Gene (H. armigera)Gr5GenBankOP251145More details about this gene see Supplementary file 1
Gene (H. armigera)Gr6GenBankOP251146More details about this gene see Supplementary file 1
Gene (H. armigera)Gr7GenBankOP251147More details about this gene see Supplementary file 1
Gene (H. armigera)Gr8GenBankOP251148More details about this gene see Supplementary file 1
Gene (H. armigera)Gr9GenBankXM_049843199More details about this gene see Supplementary file 1
Gene (H. armigera)Gr10GenBankOP251149More details about this gene see Supplementary file 1
Gene (H. armigera)Gr11GenBankOP251150More details about this gene see Supplementary file 1
Gene (H. armigera)Gr12GenBankOP251151More details about this gene see Supplementary file 1
Commercial assay or kitRNeasy Plus Universal Mini KitQiagenCat# 73404
Commercial assay or kitQ5 High-Fidelity DNA PolymeraseNEBCat# M0491
Commercial assay or kitTransStart FastPfu DNA PolymeraseTransGen BiotechCat# AP221-01
Commercial assay or kitM-MLV reverse transcriptasePromegaCat# M1701
Commercial assay or kitSYBR Premix Ex TaqTakaraCat# RR820
Commercial assay or kitmMESSAGE mMACHINE SP6AmbionCat# AM1340
Commercial assay or kitGeneArt gRNA Clean Up KitInvitrogenCat#A29377
Commercial assay or kitGeneArt gRNA Prep KitInvitrogenCat#A29377
Commercial assay or kitTrueCut Cas9 protein 2InvitrogenCat#A36498
Commercial assay or kitAnimal Tissue PCR KitTransGen BiotechCat#AD201-01
Chemical compound, drugL - (+) - ArabinoseSigma-AldrichCAS: 5328-37-0
Chemical compound, drugD - (-) - FructoseSigma-AldrichCAS: 57-48-7
Chemical compound, drugL - (-) - FucoseSigma-AldrichCAS: 2438-80-4
Chemical compound, drugD - (+) - GalactoseSigma-AldrichCAS: 59-23-4
Chemical compound, drugD - (+) - GlucoseSigma-AldrichCAS: 50-99-7
Chemical compound, drugD - (+) - MannoseSigma-AldrichCAS: 3458-28-4
Chemical compound, drugD - (+) - XyloseSigma-AldrichCAS: 58-86-6
Chemical compound, drugD - Lactose monohydrateSigma-AldrichCAS: 64044-51-1
Chemical compound, drugD - (+)-Maltose monohydrateSigma-AldrichCAS: 6363-53-7
Chemical compound, drugSucroseSigma-AldrichCAS: 57-50-1
Chemical compound, drugD - (+) - Trehalose dihydrateSigma-AldrichCAS: 6138-23-4
Chemical compound, drugSodium chlorideSigma-AldrichCAS: 7647-14-5
Chemical compound, drugPotassium chlorideSigma-AldrichCAS: 7447-40-7
Chemical compound, drugMagnesium chloride hexahydrateSigma-AldrichCAS: 7791-18-6
Chemical compound, drugHEPESSigma-AldrichCAS: 7365-45-9
Software, algorithmSAPID Tools software version 3.5Smith et al., 1990;
Software, algorithmAutospike 3.7Syntech
Software, algorithmMAFFT version 7.455Rozewicki et al., 2019
Software, algorithmtrimAI version 1.4Capella-Gutiérrez et al., 2009;
Software, algorithmIQ-tree version 6.8Nguyen et al., 2015http://iqtree.org/
Software, algorithmpCLAMP software version 10.4.2.0Axon Instruments IncRRID:SCR_011323
Software, algorithmSnapGene software version 4.3.8Insightful Sciencehttps://www.snapgene.com/
Software, algorithmSeqMan software version 7.1DNASTARhttps://www.dnastar.com/
Software, algorithmSPSS 20IBMhttps://www.ibm.com
Software, algorithmGraphPad Prism 8.2.1Dotmaticshttps://www.graphpad.com/
OtherPrimers for full-length cloning of GRsThis papersee Supplementary file 2
OtherPrimers for qRT-PCRThis papersee Supplementary file 3
OtherPrimers for Xenopus oocytes expression systemThis papersee Supplementary file 4
OtherPrimers for experiments of mutant strains establishmentThis papersee Supplementary file 5

Additional files

Supplementary file 1

GenBank accession numbers for sugar gustatory receptors used in this study.

https://cdn.elifesciences.org/articles/91711/elife-91711-supp1-v1.xlsx
Supplementary file 2

The primer sequences used in PCR for full-length cloning of GRs.

F: forward strand; R: reverse strand.

https://cdn.elifesciences.org/articles/91711/elife-91711-supp2-v1.xlsx
Supplementary file 3

The primer sequences used in qRT-PCR for GR gene expression quantification.

F: forward strand; R: reverse strand.

https://cdn.elifesciences.org/articles/91711/elife-91711-supp3-v1.xlsx
Supplementary file 4

The primer sequences used in cDNA synthesis for Xenopus oocytes expression system.

The italic sequences are protective bases, underline sequences are restriction enzymes, bold sequences are Kozak sequences. F: forward strand; R: reverse strand.

https://cdn.elifesciences.org/articles/91711/elife-91711-supp4-v1.xlsx
Supplementary file 5

The primer sequences used in the experiments of Gr10 and Gr6 mutants establishment.

F: forward strand; R: reverse strand.

https://cdn.elifesciences.org/articles/91711/elife-91711-supp5-v1.xlsx
Supplementary file 6

Summary of the CRISPR/Cas9 directed mutation rates from G0 to G2 in the establishment of Gr10 and Gr6 mutants.

https://cdn.elifesciences.org/articles/91711/elife-91711-supp6-v1.xlsx
MDAR checklist
https://cdn.elifesciences.org/articles/91711/elife-91711-mdarchecklist1-v1.docx

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