Cell-autonomous targeting of arabinogalactan by host immune factors inhibits mycobacterial growth
- Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, China
- Clinical and Translational Research Center, Shanghai Pulmonary Hospital, Tongji University School of Medicine, China
- Department of Infectious Diseases, National Medical Centre for Infectious Diseases, National Clinical Research Centre for Aging and Medicine, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, Huashan Hospital, Fudan University, China
- Abmart Inc, China
- Max Planck Institute for Infection Biology, Germany
- Max Planck Institute for Multidisciplinary Sciences, Germany
- Hagler Institute for Advanced Study, Texas A&M University, United States
- Multitude Therapeutics, China
- School of Life Science, Fudan University, China
- Department of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, China
- Central Laboratory, Shanghai Pulmonary Hospital, Tongji University School of Medicine, China
Figures
Figure 1
Galectin-9 inhibits mycobacterial growth directly.
(A) Profile of Mycobacterium tuberculosis (Mtb) H37Rv (Rv) grown at 37 °C in Middlebrook 7H9 liquid medium with different concentrations of Galectin-9 (Gal9, 0, 0.01, 0.1, 1, 10 μg/mL). Growth curve …
Figure 2
Carbohydrate recognition is essential for galectin-9-mediated inhibition of Mycobacterium tuberculosis (Mtb) growth.
(A) Growth profile of Mtb H37Rv (Rv) in Middlebrook 7H9 liquid medium with or without galectin-9 (Gal9, 10 μg/mL) and lactose (1 μg/mL). (B) Growth profile of Mtb H37Rv (Rv) in Middlebrook 7H9 …
Figure 3
Identification of anti-arabinogalactan (AG) antibodies from tuberculosis (TB) patients.
(A) Schematic presentation of ELISA assay for detecting anti-AG IgG antibodies in the serum of TB patients. (B) Linear correlation between OD and serum dilution ratio determined by ELISA assay. (C) …
Figure 4
Development of anti-arabinogalactan (AG) mAbs.
(A) Schematic presentation of mAb screening for AG specificity. (B) Representative image of chip hybridization for mAb screening. Bright spots in the bottom mark the end line of each array block. …
Figure 5
Anti-arabinogalactan (AG) antibody inhibits mycobacterial growth.
(A) Growth profile of Mycobacterium tuberculosis (Mtb) H37Rv (Rv) in Middlebrook 7H9 liquid medium with or without mAb1/mAb2 (1 μg/mL). (B) CFU of Mtb H37Rv (Rv) on Middlebrook 7H10 solid medium …
Figure 6
Proteomics profiling of the response of Mycobacterium tuberculosis (Mtb) to anti-arabinogalactan (AG) antibody.
(A) Gene ontology (GO) class of differentially expressed proteins in Mtb H37Rv treated with mAb1 (1 μg/mL) for 30 hr followed by proteomics analysis. IgG was set as control. (B) Functional …
Figure 7
Mtb cell wall modulation by anti-arabinogalactan (AG) antibodies.
(A) Morphologic characteristics for Mycobacterium tuberculosis (Mtb) H37Rv strain grown in liquid culture with or without anti-AG mAbs (1 μg/mL) observed by 2x magnifier. (B) Bacterial shape of Mtb …
